Taxonomy of European Lymnaeidae (Gastropoda: Pulmonata) in studies with the use of molecular biology techniques. I. Preliminary view on the subgenus Stagnicola Leach, 1830 on the basis of RAPD analysis

Abstract

The random amplified polymorphic DNA (RAPD) technique was used to study genomic relationships of five lymnaeid species:

Taxonomic status of Stagnicola palustris (O. F. Müller, 1774) and S. turricula (Held, 1836) (Gastropoda: Pulmonata: Lymnaeidae) in view of new molecular and chorological data

“…p. turricula (baRGues et al 2001, 2003). Our earlier studies (Rybska et al 2000a(Rybska et al , b, 2008(Rybska et al , wesołowska 2010 were not decisive and confirmed a close relationship between these two taxa. However, with our new molecular and chorological data (preliminarily presented in Pieńkowska et al 2008a, b and banasiak 2011 as well as in Rybska & lesicki 2005, respectively) we decided to verify the opposite view -that the two taxa are two distinct but closely related species, i.e.…”

“…Unexpectedly, we also found six new localities of S. palustris in south-eastern Poland (Fig. 4, (Jackiewicz 1993, FalkneR 1995, FalkneR et al 2001, GlöeR 2002, GlöeR & meieR-bRook 2003 as well as on the molecular features found in the nucleotide sequences of ITS1 and ITS2 fragments in rDNA gene (baRGues et al 2001(baRGues et al , 2006 and obtained with the use of RAPD technique (Rybska et al 2000a(Rybska et al , b, 2008. The distinct species status of these three taxa is confirmed by the results of this study.…”

“…On the other hand the three species differ in their nucleotide sequences of ITS2 fragment, as was previously shown by baRGues et al (2001,2003). established in earlier molecular analyses (baRGues et al 2001, Rybska et al 2000a It should be stressed that the genetic difference between S. palustris and S. turricula in the "barcode sequence" is also greater than the above-mentioned 2% threshold of hebeRt et al (2003, 2004a, b). Probably the low 5'COI divergence between S. palustris and S. turricula, which is much smaller than the divergences between each of these two taxa and each of the other three lymnaeid species studied in this paper, may suggest their recent origin.…”

“…Since the 1990s several different molecular methods have been intensively used to solve taxonomic problems among Lymnaeidae (RemiGio & Blair 1997a, b, baRGues & mas-coma 1997, baRGues et al 1997, RemiGio 2002, stothaRd et al 2000, tRouve et al 2000, Rybska et al 2000a, knott et al 2003, RemiGio & hebeRt 2003, caRvalho et al 2004, De Boer et al 2004, PFenninGeR et al 2006, Puslednik et al 2009, coRRea et al 2010, vinaRski 2013. As a result, some changes have been also introduced in the above classification of the genus Stagnicola.…”

The first record and occurrence of Stagnicola turricula (Held, 1836) (Gastropoda: Pulmonata: Lymnaeidae) in Upper Silesia (Southern Poland) in relation to different environmental factors

“…: Stagnicola palustris palustris and Stagnicola palustris turricula. However, their previous molecular survey (Rybska et al, 2000) confirmed the separate status of S. turricula as a species. Undoubtedly, the separate status of S. turricula as a species has also been confirmed by other European malacologists (Jackiewicz, 2000a;Falkner et al, 2001;Glöer, 2002;Fauna Europaea, 2004;Piechocki, 2008).…”