Taxonomy of Physaloptera mirandai (Nematoda: Physalopteroidea) based in three-dimensional microscopy and phylogenetic positioning

Torres, Eduardo José Lopes; Girard-Dias, Wendell; Mello, W.N.; Simões, Raquel de Oliveira; Pinto, Israel S.; Maldonado, Arnaldo; Souza, Wanderley de; Miranda, Kildare

doi:10.1016/j.actatropica.2019.04.002

Cited by 11 publications

(12 citation statements)

References 34 publications

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“…Despite the excellent resolution to characterize the surface structures in detail, conventional scanning electron microscopies (SEMs) limit the observation to the external surfaces, missing information about the internal ones. Light microscopy (LM; brightfield and fluorescence) provides information about internal structures, being complemented by SEM and the cryofracture technique (Lopes-Torres et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Over recent years, new insights into the morphology and anatomy of parasitic helminths have been generated using advanced imaging technologies, like the fluorescence and confocal microscopies (CMs) associated with electron microscopy experiments (Faulwetter et al ., 2013). Fluorescence analysis of large parasites, such as helminths, was neglected until recent advances in fluorescence stereomicroscopy using structured illumination and light sheet fluorescence microscopy, thus opening up new possibilities for the study, with the entire body of helminths in freshly fixed and hydrated samples (Lopes-Torres et al ., 2019). These findings show the importance of the association of modern multidimensional techniques for the understanding of helminth morphofunctionality, highlighting the potential of fluorescence microscopy as the basis for other experiments in the parasitology area.…”

Section: Introductionmentioning

confidence: 99%

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Eurytrema coelomaticum: updated morphology of adult worms using advanced microscopy experiments

et al. 2020

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Eurytrema coelomaticum is a digenean flatworm of ruminants that is the causative agent of eurytrematosis, a disease of veterinary health concern. Although modern techniques of morphological analysis have provided new insights about the morphology and anatomy of parasitic helminths, most studies on E. coelomaticum adults are based on conventional light microscopy. In the present study, a combined approach using brightfield, fluorescence, confocal and scanning electron microscopies (SEMs), together with the cryofracture technique, have updated morphological data on E. coelomaticum recovered from cattle in Rio de Janeiro State, Brazil. Light microscopy confirmed the presence of several structures present in the current description, such as suckers, pharynx, oesophagus, intestinal bifurcation and the cirrus-sac. Fluorescence stereomicroscopy revealed for the first time the cubic crystal protein inclusions in the forebody, which were further detailed by confocal and SEMs. Confocal microscopy provided detailed information of the muscular architecture associated with the attachment structures (suckers), digestive system (pharynx and oesophagus), egg-forming complex (ovary, Mehlis’ gland and Laurer's canal) and male reproductive system, which are similar to those found in other digenean flukes. SEM images of cryofractured parasites showed mucus and developing eggs within uterine loops. It was demonstrated that the combination of advanced tools generated complementary information, confirming the importance of experimental morphology in parasitology. Therefore, the knowledge of the adult structural organization of E. coelomaticum was improved and this work has contributed to propose new morphological criteria to evaluate the effects of antiparasitic drugs on flukes of medical and veterinary importance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Eurytrema coelomaticum: updated morphology of adult worms using advanced microscopy experiments

et al. 2020

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“…The use of SEM as a tool for helminth taxonomy has been helping for a better comprehension of the morphology of several Physaloptera spp. ( Marchiondo & Sawyer , 1978 ; Tiekotter, 1981 ; Mafra & Lanfredi, 1998 ; Lopes-Torres et al, 2009 ; Naem & Asadi, 2013 ; Chen et al, 2017 ; Ederli et al, 2018 ; Lopes-Torres et al, 2019 , Maldonado et al, 2019 ; Matias et al, 2020 ). But, studies of physalopterid nematodes using both light microscopy and SEM are scarce ( Naem & Asadi, 2013 ) and we reinforce the fact that further studies of other physalopterid species using SEM may help to define and solidify the real taxonomic value of other characters as suggested by Lopes-Torres et al (2009) .…”

Section: Discussionmentioning

confidence: 99%

“Revisiting the past”: a redescription of Physaloptera retusa (Nemata, Physalopteridae) from material deposited in museums and new material from Amazon lizards

Macedo

Willkens

Silva

et al. 2023

Rev. Bras. Parasitol. Vet.

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Physaloptera Rudolphi, 1819 is a genus of nematodes that includes approximately 100 species parasitic in vertebrates around the world. From these, approximately 30 occur in the Neotropical region, with nine reported from neotropical reptiles. Physaloptera spp. are recognized by their distinct morphology of the apical end and characters of the reproductive system. However, despite the fact that the morphological characters for species diagnosis have been firmly established, we frequently find identification problems regarding poorly detailed descriptions and poorly preserved specimens. These may lead to taxonomic incongruencies. Physaloptera retusa (Rudolphi, 1819) is the most common species of the genus and has been reported from several species of neotropical reptiles. Based on our reexaminations of nematode specimens identified as P. retusa from different museum collections, we provide a detailed redescription including the type material, voucher specimens and new specimens recovered currently and showed in this study with new morphological data obtained using light and scanning electron microscopy tools.

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“…Viannaia conspicua and V. pusilla were found in this marsupial by Freitas (1937) in Angra dos Reis, state of Rio de Janeiro, Brazil, and the latter was also reported by Thatcher (2006) in Brazil. Lent & Freitas (1937) reported P. mirandai in M. myosuros in Angra dos Reis, Brazil; Barros (2015) found this species in the states of Acre and Bahia, Lopes- Torres et al (2019) in the state of Espírito Santo, Brazil, and Tantaleán et al (2010) in Peru.…”

Section: Discussionmentioning

confidence: 99%

“…In relation to helminths, all studies published on this host have been reports of species occurrence or species descriptions (Travassos, 1913;Travassos, 1920;Travassos, 1922;Freitas, 1937;Lent & Freitas, 1937;Proença, 1937;Sarmiento, 1954;Vicente, 1966;Travassos et al, 1969;Grisi & Castro, 1974;Gomes, 1979a, b;Noronha et al, 2002;Thatcher, 2006;Tantaleán et al, 2010;Barros, 2015;Lopes-Torres et al, 2019). No studies on the helminth fauna or its community structure in this marsupial have so far been published.…”

Section: Introductionmentioning

confidence: 99%

First study on the helminth community structure of the neotropical marsupial Metachirus myosuros (Didelphimorphia, Didelphidae)

Cirino

Neto

Júnior

et al. 2020

Rev. Bras. Parasitol. Vet.

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Metachirus myosuros is a marsupial species widely distributed in South America. Despite this, there is a lack of knowledge about its helminth parasites and helminth community structure. The aims of this study were to describe the species composition and determine the parasitological parameters of helminth communities of M. myosuros in preserved areas of the Atlantic Forest, Igrapiúna, Bahia state, northeastern Brazil. Parasites were searched from 19 specimens of this marsupial (18 were infected with at least one species), counted and identified. Ten species of helminth parasites were obtained: 7 nematodes, 2 platyhelminths and 1 acanthocephalan. The most abundant species were Aspidodera raillieti, Cruzia tentaculata, Physaloptera mirandai and Viannaia conspicua (Nematoda). These species were also the only dominant ones in the component community. Male hosts had higher prevalence of P. mirandai and greater abundance of V. conspicua. We observed a relationship between host body size and helminth abundance in both male and female hosts, and between host body size and helminth species richness in female hosts. This was the first study to analyze the helminth fauna and helminth community structure of M. myosuros. This was the first report of occurrences of A. raillieti and Didelphonema longispiculata in M. myosuros.

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Taxonomy of Physaloptera mirandai (Nematoda: Physalopteroidea) based in three-dimensional microscopy and phylogenetic positioning

Cited by 11 publications

References 34 publications

Eurytrema coelomaticum: updated morphology of adult worms using advanced microscopy experiments

Eurytrema coelomaticum: updated morphology of adult worms using advanced microscopy experiments

“Revisiting the past”: a redescription of Physaloptera retusa (Nemata, Physalopteridae) from material deposited in museums and new material from Amazon lizards

First study on the helminth community structure of the neotropical marsupial Metachirus myosuros (Didelphimorphia, Didelphidae)

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