Taxonomy of some recently described avianpasteurella/ actinobacillus‐likeorganisms as indicated by deoxyribonucleic acid relatedness

Inter- and Intrafamilial Similarities of rRNA Cistrons of the Pasteurellaceae

“…The relative closeness of these two genera is also obvious from DNA hybridization data (41). The genomic relationships of several Actinobacillus-like organisms of avian origin were examined by Piechulla et al (53). On the basis of DNA homology data Escande et al (29) suggested that the genus Actinobacillus should be limited to the following species: Actinobacillus lignieresii, Actinobacillus suis, Actinobacillus capsulatus, an unnamed equine Actinobacillus species represented by strain CCM 5500, and the generically misnamed organism [Pasteurella] ureae.…”

“…Most strains belonging to the family Pasteurellaceae were grown primarily on chocolate agar (tryptic soy agar [Difco Laboratories] which after sterilization was supplemented with 5% [voVvol] sheep blood and was kept at 80°C for 15 min before pouring) in a moist atmosphere containing 5 to 10% carbon dioxide at 36°C. Mass cultures were propagated in Difco Proteose peptone medium (78) supplemented with yeast extract (0.5%, wthol), NAD, hematin, or thiamine monophosphate as previously described (12,47,(53)(54)(55).…”

Avian pasteurellosis: Taxonomy of the organisms involved and aspects of pathogenesis

“…Organisms provisionally named taxon 2 and taxon 3 were first described by Bisgaard (1982). These organisms were subsequently shown to form a large distinct group which seems to represent a new genus with several species within the family Pasteurellaceae (Piechulla et al, 1985b). Additional strains have been reported by Beichel (1986).…”

“…Avian isolates named fP.J-haemolytica-like/[A.J salpingitidis form a large heterogeneous genus-like group which has been located in the close vicinity of the genus Actinobacillus by Piechulla et al (1985b). Organisms provisionally named taxon 2 and taxon 3 were first described by Bisgaard (1982).…”

“…Subsequently, Ryll et al (1991) Avian Actinobacillus-like organisms (taxon 26), which contains four biovars, appeared heterogeneous at species level by DNA:DNA hybridizations (Piechulla et al, 1985b). These organisms are normally isolated from the upper respiratory tract of web-footed birds and may cause sinusitis, conjunctivitis and septicaemia.…”