Taxonomy of Southeast Asian-Australasian grapevine leaf rust fungus and its close relatives

Ono, Yoshitaka; Okane, Izumi; Chatasiri, Sinchai; Pota, Siriporn; Unartngam, Jintana; Ayawong, Chanjira; Nguyen, Hien Thi; Le, Cham Thi Mai

doi:10.1007/s11557-020-01607-2

Cited by 8 publications

(9 citation statements)

References 42 publications

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“…Phylogenetic analysis showed that all 50 isolates from the south‐central region clustered together in a subclade with the Thai population and two Brazilian isolates, previously identified as N . tropicalis (Okane and Ono, 2018; Ono et al ., 2020). Isolates from the north‐east region formed a clade with isolates from Japan and Taiwan, described as N .…”

Section: Discussionmentioning

confidence: 99%

“…(Pota et al ., 2015; Okane and Ono, 2018), later renamed as N . tropicalis (Ono et al ., 2020). Sequences of the ITS2‐D1/D2 region from N .…”

Section: Discussionmentioning

confidence: 99%

“…Most recently, the Neophysopella sp. was named after a comprehensive taxonomic study as Neophysopella tropicalis (Ono et al ., 2020). Although AGLR is a serious disease affecting Brazilian vineyards, little is known about its aetiology in the country.…”

Section: Introductionmentioning

confidence: 99%

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Identification and pathogenicity of Neophysopella species associated with Asian grapevine leaf rust in Brazil

2020

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Grapevine rust has been associated with different causal agents belonging to the genus Phakopsora, which were recently transferred to the new genus Neophysopella (Ji et al., 2019). In North and Central America, the disease is caused by Neophysopella uva (Buriticá, 1994), which occurs on various Vitis species, and by Neophysopella muscadiniae only on Vitis rotundifolia (Buriticá, 1999). In Asia, grapevine rust is associated with Neophysopella ampelopsidis (Leu, 1988), which was considered the causal agent of rust in several genera of the family Vitaceae up to the year 2000. To elucidate the taxonomy of the N. ampelopsidis species complex, experimental inoculations on different vitaceous hosts (Ampelopsis, Parthenocissus, and Vitis) and comparative spore morphology have been performed (Ono, 2000). Based on these results, the species complex was split into three distinct species: N. ampelopsidis, Neophysopella vitis, and Neophysopella euvitis, forming uredinial and telial stages on Ampelopsis, Parthenocissus, and Vitis, respectively (Ono, 2000). Thus, the causal agent of Asian grapevine leaf rust (AGLR) was recognized as N. euvitis.

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Section: Discussionmentioning

confidence: 99%

“…(Pota et al ., 2015; Okane and Ono, 2018), later renamed as N . tropicalis (Ono et al ., 2020). Sequences of the ITS2‐D1/D2 region from N .…”

Section: Discussionmentioning

confidence: 99%

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Identification and pathogenicity of Neophysopella species associated with Asian grapevine leaf rust in Brazil

2020

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“…Individual specimen data are listed in the Taxonomy section. Morphological examinations, both by light microcopy and scanning electron microscopy, were undertaken by the methods described by Ono et al (2020a , b ).…”

Section: Specimens and Morphological Examinationmentioning

confidence: 99%

Discovery of teliospores of a Cape jasmine rust fungus, <i>Hemileia gardeniae-floridae</i> (<i>Pucciniales</i>), and its occurrence in Thailand

et al. 2021

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The genus Hemileia (Berkeley & Broome, 1869) (Zaghouaniaceae, Pucciniales) has captured the attention of people all over the world because the type species of the genus, H. vastatrix Berk. & Broome, commonly known as coffee leaf rust fungus or orange coffee rust fungus, is one of the most devastating invasive fungal pathogens of economically important commodity crops (Avelino et al., 2015;Anonymous, 2016;McCook, 2019). Hemileia is comprised of some forty named species (Ritschel, 2005;Judith & Rossman, 2014). The genus is characterized by suprastomatal sori and hemispherical or kidney-shaped urediniospores with dorsal echinulate and ventral smooth surfaces. Because of these unique uredinial characteristics, about one-third of species named under the genus name Hemileia are based only on the uredinial morph. Current International Code of Nomenclature for algae, fungi, and plants (the Shenzhen Code; Turland et al., 2018) accepts the priority of names of Hemileia species even if they are based on the uredinial anamorph.Species of Hemileia occur on forty genera in nine vascular plant families and mostly in subtropical and tropical regions worldwide (Ritschel, 2005). Among those plant families, each of two plant families, Apocynaceae and Rubiaceae, harbors about one-third of Hemileia species. In addition to the fact that one-third of species are anamorphic, because of often faced difficulty in identification of host species, particularly in old sterile specimens, and because of poor morphological features available as taxonomic characters, species delimitation and their classification in Hemileia have remained indecisive.This study aimed at confirming generic assignment a poorly understood hemileioid anamorphic fungus on Cape jasmine (Gardenia jasminoides J. Ellis) first collected in Taiwan and named as H. gardeniae-floridae by Sawada (1931) and identifying a hemileioid fungus on Golden gardenia, G. sootepensis Hutch., found in Thailand. Specimens and morphological examinationSawada (1931) did not designate the type for H. gardeniae-floridae in the original description. The type specimen was discovered at the Mycological Herbarium, the University of Tsukuba (TSH), on which specimen label, "Type" was clearly hand-written by K. Sawada (Fig. 1A). The specimen designated as the type of Uredo gardeniae-floridae (Sawada) Hirats. f. (Hiratsuka, 1960) and additional two specimens on G. jasminoides deposited at TSH were also examined. Three specimens on G. sootepensis from Thailand deposited at the Herbarium of Systematic Mycology, Ibaraki University (IBAR) were compared to those on G. jasminoides for taxonomic Discovery of teliospores of a Cape jasmine rust fungus, Hemileia gardeniaefloridae (Pucciniales), and its occurrence in Thailand

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“…AGLR is caused by N. montana, N. meliosmae-myrianthae, and N. tropicalis (Chatasiri and Ono, 2008;Ono et al, 2012Ono et al, , 2020. AGLR (N. tropicalis) was reported for the first time in south-east Brazil in 2001 and no alternate host is known (Tessmann et al, 2004).…”

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confidence: 99%

Early detection of Neophysopella tropicalis in grapevine leaves and on spore traps by qPCR

et al. 2020

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Neophysopella tropicalis, one of the causal agents of Asian grapevine leaf rust (AGLR), can cause severe epidemics in Brazil that lead to yield losses in commercial vineyards. An early detection of the pathogen by air sampling of urediniospores on spore traps or in symptomless leaves would be valuable to multiple studies, such as epidemics modelling, risk forecasting, monitoring of pathogen introductions in rust‐free areas, and predicting the beginning of epidemics. This study developed a quantitative PCR (qPCR) protocol to quantify N. tropicalis urediniospores attached to adhesive tapes and in grapevine leaves before symptom appearance. A specific primer pair was designed based on the internal transcribed spacer (ITS) sequence region of the AGLR pathogen. Standard amplification curves using genomic DNA from urediniospores of N. tropicalis and from urediniospores attached to adhesive tapes were established. Grapevine leaves inoculated with N. tropicalis were collected at 2, 5, and 7 days postinoculation (dpi). One primer pair (580F/720R) amplified a 140 bp product in all AGLR isolates but did not amplify products of other rust genera, such as Phakopsora, Puccinia, Hemileia, Tranzschelia, Cerotelium, and Coleosporium. As little as 0.1 pg DNA and 10 urediniospores of N. tropicalis attached to adhesive tapes could be detected. qPCR enabled the detection of the pathogen as early as 2 dpi, before symptom appearance. This method can be used to monitor N. tropicalis inoculum in grapevine‐growing areas and to quantify symptomless infections of the AGLR pathogen.

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Taxonomy of Southeast Asian-Australasian grapevine leaf rust fungus and its close relatives

Cited by 8 publications

References 42 publications

Identification and pathogenicity of Neophysopella species associated with Asian grapevine leaf rust in Brazil

Identification and pathogenicity of Neophysopella species associated with Asian grapevine leaf rust in Brazil

Discovery of teliospores of a Cape jasmine rust fungus, <i>Hemileia gardeniae-floridae</i> (<i>Pucciniales</i>), and its occurrence in Thailand

Early detection of Neophysopella tropicalis in grapevine leaves and on spore traps by qPCR

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