Taylor-Couette Vortex Flow in Enzymatic Reactors

Giordano, Roberto; Giordano, Raquel de Lima Camargo

doi:10.1007/978-1-59745-053-9_28

Cited by 4 publications

(6 citation statements)

References 20 publications

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“…Visually, the biocatalyst was uniformly distributed along the reactor (see Figure 6), as reported elsewhere. 37,38,40,44,45…”

Section: Resultsmentioning

confidence: 99%

“…The same apparatus also may be used as an adsorption unit, with the fluidization of suspended particles of adsorbent sustained by the vortices. A comprehensive review of all applications is beyond our scope here; however, some examples may be found elsewhere. − Nevertheless, the application of a VFR for an enzymatic synthesis with simultaneous crystallization of the products has not been reported yet, to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

“…A comprehensive review of all applications is beyond our scope here; however, some examples may be found elsewhere. [34][35][36][37][38][39][40][41][42][43][44][45] Nevertheless, the application of a VFR for an enzymatic synthesis with simultaneous crystallization of the products has not been reported yet, to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

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Nonconventional Reactor for Enzymatic Synthesis of Semi-Synthetic β-Lactam Antibiotics

Ferreira

Giordano

2007

Ind. Eng. Chem. Res.

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The enzymatic synthesis of β-lactam semi-synthetic antibiotics has been receiving increasing attention as a green-chemistry alternative for the industrial production of these drugs, because mild reaction conditions may be used. A nonconventional fed-batch reactor is presented here, using a bi-disperse gel matrix for immobilization of the enzyme penicillin G acylase (PGA) [EC 3.5.1.11]. The catalyst particles are suspended within Taylor−Couette vortices, performing the kinetically controlled synthesis of ampicillin (AMP) from phenylglycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). This is a serial−parallel set of reactions, where the desired product (AMP) is the intermediate species, and a high selectivity is essential for the process economics. With this objective, AMP should be precipitated, withdrawing the antibiotic from the liquid phase and reducing its hydrolysis. One key point is to protect the physical integrity of the catalyst within this environment. To avoid damages to the catalyst particle caused by conventional impellers, while preserving a good mixing, Taylor−Couette flow was used. In addition, a convenient biocatalyst matrix was developed, to allow easy separation between the crystals and the enzyme support. A bench-scale (50 mL) Taylor vortex flow reactor (VFR), with a radius ratio of η = 0.27 and operating in fed-batch mode, was used for proof of the concept. To sustain homogeneous fluidization of the biocatalyst, the VFR operated with a rotational Reynolds number of Re = 5605, within the turbulent Taylor vortices flow region. With this reactor−catalyst ensemble, 100% activity and complete physical integrity of the particles were sustained after 200 h of operation.

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“…Visually, the biocatalyst was uniformly distributed along the reactor (see Figure 6), as reported elsewhere. 37,38,40,44,45…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nonconventional Reactor for Enzymatic Synthesis of Semi-Synthetic β-Lactam Antibiotics

Ferreira

Giordano

2007

Ind. Eng. Chem. Res.

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“…The transition of these flow patterns largely depends on the radius ratio of the inner and outer cylinder and on the fluid viscosity. Many different ways of analyzing and quantifying the corresponding flow patterns are described in the literature 10, 14–19. However, the problems with these flow instabilities and transitions between flow patterns are complex and cannot be solved by theory alone 12.…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic efficiency of Chlorella sorokiniana in a turbulently mixed short light‐path photobioreactor

Kliphuis

Winter

Vejrazka

et al. 2010

Biotechnology Progress

139

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To be able to study the effect of mixing as well as any other parameter on productivity of algal cultures, we designed a lab-scale photobioreactor in which a short light path (SLP) of (12 mm) is combined with controlled mixing and aeration. Mixing is provided by rotating an inner tube in the cylindrical cultivation vessel creating Taylor vortex flow and as such mixing can be uncoupled from aeration. Gas exchange is monitored on-line to gain insight in growth and productivity. The maximal productivity, hence photosynthetic efficiency, of Chlorella sorokiniana cultures at high light intensities (1,500 micromol m(-1) s(-1)) was investigated in this Taylor vortex flow SLP photobioreactor. We performed duplicate batch experiments at three different mixing rates: 70, 110, and 140 rpm, all in the turbulent Taylor vortex flow regime. For the mixing rate of 140 rpm, we calculated a quantum requirement for oxygen evolution of 21.2 mol PAR photons per mol O(2) and a yield of biomass on light energy of 0.8 g biomass per mol PAR photons. The maximal photosynthetic efficiency was found at relatively low biomass densities (2.3 g L(-1)) at which light was just attenuated before reaching the rear of the culture. When increasing the mixing rate twofold, we only found a small increase in productivity. On the basis of these results, we conclude that the maximal productivity and photosynthetic efficiency for C. sorokiniana can be found at that biomass concentration where no significant dark zone can develop and that the influence of mixing-induced light/dark fluctuations is marginal.

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“…The enzymatic maltose production was performed in a batch Taylor vortex flow reactor (VFR) [90] with radius ratio (η = Ri/Re) of 0.24, aspect ratio (Γ = L/d) of 6.32 and inner cylinder rotation rate (ω) of 900 rpm, using 50.0 mL of commercial starch solution or residual starch solution extracted from cassava bagasse and 36.0 U/L of free or CLEA of β-amylase, at pH and temperature values of maximum stability (determined as described before). The starch conversion was followed by the increase of maltose concentration during the reaction time.…”

Section: Maltose Productionmentioning

confidence: 99%

Maltose Production Using Starch from Cassava Bagasse Catalyzed by Cross-Linked β-Amylase Aggregates

et al. 2018

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Barley β-amylase was immobilized using different techniques. The highest global yield was obtained using the cross-linked enzyme aggregates (CLEA) technique, employing bovine serum albumin (BSA) or soy protein isolate (SPI) as feeder proteins to reduce diffusion problems. The CLEAs produced using BSA or SPI showed 82.7 ± 5.8 and 53.3 ± 2.4% global yield, respectively, and a stabilization effect was observed upon immobilization at neutral pH value, e.g., after 12 h at 55 • C, the free β-amylase is fully inactivated, while CLEAs retained 25 and 15% of activity (using BSA and SPI, respectively). CLEA using SPI was selected because of its easier recovery, being chosen to convert the residual starch contained in cassava bagasse into maltose. This biocatalyst permitted to reach almost 70% of maltose conversion in 4 h using 30.0 g/L bagasse starch solution (Dextrose Equivalent of 15.88) and 1.2 U of biocatalyst per gram of starch at pH 7.0 and 40 • C. After 4 reuses (batches of 12 h) the CLEA using SPI maintained 25.50 ± 0.01% of conversion due to the difficulty of recovering.

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Taylor-Couette Vortex Flow in Enzymatic Reactors

Cited by 4 publications

References 20 publications

Nonconventional Reactor for Enzymatic Synthesis of Semi-Synthetic β-Lactam Antibiotics

Nonconventional Reactor for Enzymatic Synthesis of Semi-Synthetic β-Lactam Antibiotics

Photosynthetic efficiency of Chlorella sorokiniana in a turbulently mixed short light‐path photobioreactor

Maltose Production Using Starch from Cassava Bagasse Catalyzed by Cross-Linked β-Amylase Aggregates

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