TBX3 Directs Cell-Fate Decision toward Mesendoderm

Weidgang, Clair E.; Russell, Ronan; Tata, Purushothama Rao; Kühl, Susanne J.; Illing, Anett; Müller, Martin; Lin, Qiong; Brunner, Cornelia; Boeckers, Tobias M.; Bauer, Kerstin; Kartikasari, Apriliana E. R.; Guo, Yu; Radenz, Melanie; Bernemann, Christof; Weiß, Matthias; Seufferlein, Thomas; Zenke, Martin; Iacovino, Michelina; Kyba, Michael; Schöler, Hans R.; Kühl, Michael; Liebau, Stefan; Kleger, Alexander

doi:10.1016/j.stemcr.2014.04.011

Cited by 23 publications

(30 citation statements)

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“…7B). It is necessary for mesendoderm and extraembryonic endoderm (ExEn) differentiation, as well as for the suppression of TE and ectoderm differentiation (Lu et al, 2011;Weidgang et al, 2013). Mechanistically, Tbx3 functions through direct binding and epigenetic modification of histones on the promoter of Gata6, which encodes an essential regulator of ExEn (Lu et al, 2011).…”

Section: T-box Genes and Stem Cell Biologymentioning

confidence: 99%

The T-box gene family: emerging roles in development, stem cells and cancer

2014

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The T-box family of transcription factors exhibits widespread involvement throughout development in all metazoans. T-box proteins are characterized by a DNA-binding motif known as the T-domain that binds DNA in a sequence-specific manner. In humans, mutations in many of the genes within the T-box family result in developmental syndromes, and there is increasing evidence to support a role for these factors in certain cancers. In addition, although early studies focused on the role of T-box factors in early embryogenesis, recent studies in mice have uncovered additional roles in unsuspected places, for example in adult stem cell populations. Here, I provide an overview of the key features of T-box transcription factors and highlight their roles and mechanisms of action during various stages of development and in stem/progenitor cell populations.

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Section: T-box Genes and Stem Cell Biologymentioning

confidence: 99%

The T-box gene family: emerging roles in development, stem cells and cancer

2014

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“…2B are listed in Supplementary Table S4. To gain functional insights from these gene expression data, gene set enrichment analysis (GSEA) was conducted using predefined gene sets (ectoderm, mesoderm, and endoderm) [27][28][29] and a customized R script for GSEA. The datasets were submitted to Gene Expression Omnibus database (www.ncbi.nlm.nih.gov/geo).…”

Section: Cell Growth Rate and Mtt Assaymentioning

confidence: 99%

Dysregulation of Mitochondrial Functions and Osteogenic Differentiation in Cisd2-Deficient Murine Induced Pluripotent Stem Cells

Tsai¹,

Chien

Chuang

et al. 2015

Stem Cells and Development

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“…Tbx3 is expressed in the anterior neural plate at eye field stages (Li et al, 1997;Wong et al, 2002;Zuber et al, 2003;Weidgang et al, 2013). We used in situ hybridization to more precisely define the tbx3 expression pattern (Fig.…”

Section: Tbx3 Is Required For Normal Eye Formationmentioning

confidence: 99%

Tbx3 represses bmp4 expression and, with Pax6, is required and sufficient for retina formation

et al. 2016

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Vertebrate eye formation begins in the anterior neural plate in the eye field. Seven eye field transcription factors (EFTFs) are expressed in eye field cells and when expressed together are sufficient to generate retina from pluripotent cells. The EFTF Tbx3 can regulate the expression of some EFTFs; however, its role in retina formation is unknown. Here, we show that Tbx3 represses bmp4 transcription and is required in the eye field for both neural induction and normal eye formation in Xenopus laevis. Although sufficient for neural induction, Tbx3-expressing pluripotent cells only form retina in the context of the eye field. Unlike Tbx3, the neural inducer Noggin can generate retina both within and outside the eye field. We found that the neural and retina-inducing activity of Noggin requires Tbx3. Noggin, but not Tbx3, induces Pax6 and coexpression of Tbx3 and Pax6 is sufficient to determine pluripotent cells to a retinal lineage. Our results suggest that Tbx3 represses bmp4 expression and maintains eye field neural progenitors in a multipotent state; then, in combination with Pax6, Tbx3 causes eye field cells to form retina.

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TBX3 Directs Cell-Fate Decision toward Mesendoderm

Cited by 23 publications

References 0 publications

The T-box gene family: emerging roles in development, stem cells and cancer

The T-box gene family: emerging roles in development, stem cells and cancer

Dysregulation of Mitochondrial Functions and Osteogenic Differentiation in Cisd2-Deficient Murine Induced Pluripotent Stem Cells

Tbx3 represses bmp4 expression and, with Pax6, is required and sufficient for retina formation

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