Tc-99m White Cell Scintigraphy in Suspected Acute Infection

Southee, Andrew E.; Lee, Kenneth J.; McLaughlin, Andrew F.; Borham, Peter; Bautovich, George J.; Morris, John G.

doi:10.1097/00003072-199002000-00001

Cited by 14 publications

(6 citation statements)

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“…Hence, by mapping the location of Sn within neutrophils we can infer the location of Tc, that is, with XFM we can use Sn as a "tracer" for the location of Tc. In the clinical setting TcSn colloid has consistently proven efficacious in detecting focal infection [4][5][6][7][8]. Some studies have reported pessimistically on TcSn colloid leukocyte labelling efficiency [9]; however, such studies may not use the standard labelling method.…”

Section: Discussionmentioning

confidence: 99%

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Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM)

et al. 2011

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Synchrotron-based X-ray fluorescence microscopy (XFM) can localise chemical elements at a subcellular level. 99mTechnetium stannous (TcSn) colloid is taken up by phagocytes via a Complement Receptor 3 mediated phagocytic process. In the current study, XFM was used to examine the intracellular trafficking of TcSn colloid in neutrophils. XFM was performed on TcSn colloid, and neutrophils labelled with TcSn colloid, in whole blood. We developed a set of pixel by pixel analysis and mapping techniques incorporating cluster analysis that allowed us to differentiate neutrophils and artefactual contaminants, and we examined the changes in element distribution that accompany neutrophil phagocytosis of TcSn colloid. Sn became associated with half the neutrophils. Within cells, Sn colocalised with iron (Fe) and sulphur (S), and was negatively associated with calcium (Ca). Despite the high sensitivity of XFM, Tc was not detected. XFM can help clarify the intracellular processes that accompany neutrophil phagocytosis. The subcellular colocalisation of Sn with Fe is consistent with fusion of the colloid-containing phagosome with neutrophil Electronic supplementary material The online version of this article

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Section: Discussionmentioning

confidence: 99%

“…TcSn colloid (LLK Radpharm Australia) is a commercially available radiopharmaceutical used to label phagocytic leukocytes to image sites of infection [4][5][6][7][8]. When fresh colloid is examined immediately after manufacture it is found to consist of clumps of particles measuring on the order of several micrometers [9,10].…”

Section: Introductionmentioning

confidence: 99%

Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM)

et al. 2011

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“…Tc-labled stannous colloids ( 99m Tc-SnC) is a radiolabeling technique to label neutrophils and monocytes for imaging of inflammation and spatial localization of infection for gram-positive as well as gram-negative bacteria [21][22][23][24][25][26].…”

Section: M Tc-stannous Colloid 99mmentioning

confidence: 99%

Synthetic approaches to radiochemical probes for imaging of bacterial infections

Dutta

Naicker

Ebenhan

et al. 2017

European Journal of Medicinal Chemistry

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This present review provides an account on the available synthetic strategies employed to radiolabel commercial and potential bacteria-selective probes for tomographic imaging. These molecular probes encompass leukocytes, antibodies, small molecules, peptides, antibiotics, macrolides, vitamins, oligomers and siderophores. Although this technique has shown to be a valuable tool for non-invasive infection imaging, more development is required to create easy-to-radiolabel kit solutions/procedures for the preparation of the probes.

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“…Although 99m TcSnC is not approved for human use in the United States, 10 it is approved in Australia for investigation of inflammatory disorders in people 11–17 . The main advantage of 99m TcSnC over other in vitro labeling radiopharmaceuticals is its ability to radiolabel leukocytes in whole blood, thereby obviating the need for separation of leukocytes.…”

Section: Introductionmentioning

confidence: 99%

“…The main advantage of 99m TcSnC over other in vitro labeling radiopharmaceuticals is its ability to radiolabel leukocytes in whole blood, thereby obviating the need for separation of leukocytes. This ensures that the labeling procedure is simple, fast, and safe 11–17 . 99m TcSnC is also less expensive than other radiopharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

IN VITRO EVALUATION OF FELINE LEUKOCYTES RADIOLABELED IN WHOLE BLOOD WITH ^99MTC STANNOUS COLLOID

Abushhiwa

Salehi

Whitton

et al. 2009

Vet Radiology Ultrasound

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Technetium-99m stannous colloid (9mTcSnC) has been used to radiolabel human leukocytes to investigate various inflammatory disorders. We investigated the in vitro behavior of feline leukocytes labeled in whole blood with 99mTcSnC. Heparinized blood samples were collected from healthy cats and divided into control and test aliquots. The latter were labeled with 99mTcSnC using a standard procedure. Leukocyte viability was determined for each sample using a trypan blue exclusion test. Labeling efficiency was determined for test aliquots. Test aliquots were layered onto Histopaque-1077 and centrifuged before measurement of radioactivity of the blood components. Leukocytes from radiolabeled and control samples were washed and incubated with opsonized zymosan particles to allow assessment of phagocytic function. Aliquots were taken from radiolabeled feline leukocyte samples at 1, 3, 4, and 7 h postlabelling. After centrifugation of each aliquot, radioactivity of the supernatant and pellet was measured and the labeling retention determined. Leukocyte viability in both radiolabeled and control samples was > 98%. The labeling efficiency was 95.2 +/- 0.14%. The distribution of radioactivity in feline blood was found to be 3.4 +/- 0.18% in plasma, 39.0 +/- 0.37% in erythrocytes, and 57.6 +/- 0.38% in leukocytes. Labeled feline leukocytes had phagocytic activity of 90.9 +/- 0.18% (control 91.3 +/- 0.15%). The radiolabeled leukocytes retained 93.4 +/- 0.19% of the radioactivity up to 7h postlabeling. 99TcSnC efficiently labeled feline leukocytes with no effect on viability and minimal effect on phagocytic function. The percentage retention of radioactivity by the leukocytes was still high at 7h postlabeling.

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Tc-99m White Cell Scintigraphy in Suspected Acute Infection

Cited by 14 publications

References 0 publications

Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM)

Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM)

Synthetic approaches to radiochemical probes for imaging of bacterial infections

IN VITRO EVALUATION OF FELINE LEUKOCYTES RADIOLABELED IN WHOLE BLOOD WITH ^99MTC STANNOUS COLLOID

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Tc-99m White Cell Scintigraphy in Suspected Acute Infection

Cited by 14 publications

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Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM)

Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM)

Synthetic approaches to radiochemical probes for imaging of bacterial infections

IN VITRO EVALUATION OF FELINE LEUKOCYTES RADIOLABELED IN WHOLE BLOOD WITH 99MTC STANNOUS COLLOID

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IN VITRO EVALUATION OF FELINE LEUKOCYTES RADIOLABELED IN WHOLE BLOOD WITH ^99MTC STANNOUS COLLOID