Tc1 transposase of Caenorhabditis elegans is an endonuclease with a bipartite DNA binding domain.

Vos, J. Chris; Plasterk, Ronald H.A.

doi:10.1002/j.1460-2075.1994.tb06959.x

Cited by 96 publications

(102 citation statements)

References 32 publications

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“…Specific substrate recognition is mediated by an N-terminal, bipartite DNA-binding domain of the transposase (Fig. 2) (6,7,8). This DNA-binding domain has been proposed to consist of two helix-turn-helix (HTH) motifs, similar to the paired domain of some transcription factors in both amino acid sequence and structure (2,8,9).…”

Section: The Transposase the Dna-binding Domainmentioning

confidence: 99%

“…2) (6,7,8). This DNA-binding domain has been proposed to consist of two helix-turn-helix (HTH) motifs, similar to the paired domain of some transcription factors in both amino acid sequence and structure (2,8,9). The modular paired domain has evolved versatility in binding to a range of different DNA sequences through various combinations of its subdomains (PAI+RED) (10).…”

Section: The Transposase the Dna-binding Domainmentioning

confidence: 99%

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Sleeping Beauty Transposition

Ivics

Izsvák

2015

Microbiol Spectr

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Sleeping Beauty (SB) is a synthetic transposon that was constructed based on sequences of transpositionally inactive elements isolated from fish genomes. SB is a Tc1/mariner superfamily transposon following a cut-and-paste transpositional reaction, during which the element-encoded transposase interacts with its binding sites in the terminal inverted repeats of the transposon, promotes the assembly of a synaptic complex, catalyzes excision of the element out of its donor site, and integrates the excised transposon into a new location in target DNA. SB transposition is dependent on cellular host factors. Transcriptional control of transposase expression is regulated by the HMG2L1 transcription factor. Synaptic complex assembly is promoted by the HMGB1 protein and regulated by chromatin structure. SB transposition is highly dependent on the nonhomologous end joining (NHEJ) pathway of double-strand DNA break repair that generates a transposon footprint at the excision site. Through its association with the Miz-1 transcription factor, the SB transposase downregulates cyclin D1 expression that results in a slowdown of the cell-cycle in the G1 phase, where NHEJ is preferentially active. Transposon integration occurs at TA dinucleotides in the target DNA, which are duplicated at the flanks of the integrated transposon.

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Section: The Transposase the Dna-binding Domainmentioning

confidence: 99%

Section: The Transposase the Dna-binding Domainmentioning

confidence: 99%

Sleeping Beauty Transposition

Ivics

Izsvák

2015

Microbiol Spectr

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“…TA was mutated to CG, CA to TG, and GT to AC, respectively. In the transposase binding site mutation, the Bali and EcoRV sites are mutated to TCCCCA and GGGCCC, respectively (see Vos and Plasterk 1994). bacterial transposons Tn7 (Bainton et al 1991(Bainton et al , 1993 and TnlO (Bender and Kleckner 1986) as well as the Drosophila P element (Kaufman and Rio 1992).…”

Section: I N I M Al Cis-requirem En Tsmentioning

confidence: 99%

“…The element-encoded proteins share a homologous catalytic domain with bacterial transposases and retroviral integrases (Doak et al 1994). Tcl from C. e/egans is a 1612-bp-long transposon that has 54-bp inverted repeats flanking a gene encoding a 343-amino-acid transposase (Emmons et al 1983;Rosenzweig et al 1983;Vos et al 1993), which binds to the inverted repeats (Vos et al 1993;Vos and Plasterk 1994). The conserved hexanucleotide sequence, TACAGT, at the extreme termini of the element is not part of the transposase binding site but is thought to play a role in catalysis of the transposition reaction (Vos and Plasterk 1994).…”

mentioning

confidence: 99%

Transposase is the only nematode protein required for in vitro transposition of Tc1.

Vos¹,

Baere²,

Plasterk³

1996

Genes Dev.

176

132

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The Tcl element of Caenorhabditis elegans is a member of the most widespread class of DNA transposons known in nature. Here, we describe efficient and precise transposition of Tcl in a cell-free system. Tcl appears to jump by a cut-and-paste mechanism of transposition. The terminal 26 bp of the Tcl terminal repeats together with the flanking TA sequence are sufficient for transposition. The target site choice in vitro is similar to that in vivo. Transposition is achieved with an extract prepared from nuclei of transgenic nematodes that overexpress Tcl transposase but also by recombinant transposase purified from Escherichia coli. The simple reaction requirements explain why horizontal spread of Tcl/mariner transposons can occur.They also suggest that Tcl may be a good vector for transgenesis of diverse animal species.

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“…The TC1-like element is a member of the TC1/mariner superfamily of eukaryotic transposons that achieve their mobility via DNA intermediates [23][24][25]. The Xena element is a retrotransposon which spreads via an RNA intermediate.…”

Section: Transposable Elementsmentioning

confidence: 99%

Clustering of C‐Type Lectin Natural Killer Receptor‐Like Loci in the Bony Fish Oreochromis niloticus

et al. 2004

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The genome of the cichlid (teleost) fish Oreochromis niloticus contains a set of genes which encode group V C-type lectin proteins homologous to the mammalian NKG2/CD94 family of natural killer (NK) cell receptors. To determine the genomic organization of these killer cell-like receptor (KLR) genes, an O. niloticus BAC library was screened with a cDNA probe derived previously from an expressed sequence tag of the related cichlid species Paralabidochromis chilotes. Four distinct KLR-bearing BAC clones were analysed, three of which could be assembled into a contig. One of the clones was sequenced in its entirety, whereas the others were partially sequenced to identify the KLR loci borne by them. Altogether, 28 distinct KLR loci were identified, of which at least 26 occupy a single chromosomal region, the KLR complex. One half of the loci appear to be occupied by pseudogenes. Compared to the human NK cell receptor complex, the Oreochromis KLR complex is more compact and, apart from transposons, appears to contain only KLR loci. The gene density of the complex is one KLR locus per 18 kb of sequence. All the KLR loci constituting the complex are derived from a single most recent common ancestor, which is estimated to have existed 7.7 million years ago. The 180 kb of the determined sequence is a mosaic of blocks of similar segments reflecting a complex history of duplications, deletions and rearrangements. The transposons found in the sequenced part belong to the TC1, Xena, CR1 and TX1 families.

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Tc1 transposase of Caenorhabditis elegans is an endonuclease with a bipartite DNA binding domain.

Cited by 96 publications

References 32 publications

Sleeping Beauty Transposition

Sleeping Beauty Transposition

Transposase is the only nematode protein required for in vitro transposition of Tc1.

Clustering of C‐Type Lectin Natural Killer Receptor‐Like Loci in the Bony Fish Oreochromis niloticus

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