2007
DOI: 10.1007/s00441-007-0527-y
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TCam-2 but not JKT-1 cells resemble seminoma in cell culture

Abstract: Of all malignancies diagnosed in men between 17 and 45 years of age, 60% are germ cell tumors (GCT). GCT arise from carcinoma in situ cells, which are thought to originate from a transformed fetal germ cell, the gonocyte. Seminoma together with embryonal carcinoma represent the most frequent subtypes of GCT. However, the nature of the molecular pathways involved in seminoma formation remains elusive. Therefore, analysis of appropriate cell culture systems is an important prerequisite for further understanding … Show more

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Cited by 75 publications
(131 citation statements)
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“…Alternatively, the data for JKT-1, together with experiments from us and others, further indicate that JKT-1 does not (or no longer) display seminoma-like characteristics. 25,26 To address the question whether NANOG expression correlates to methylation of the CpGs in the NRR in GCTs and derived cell lines, we performed qRT-PCR analysis. NANOG expression levels were high in seminomas and embryonal carcinomas, while low and absent in teratomas, yolk sac tumors, choriocarcinomas and mixed non-seminomas (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Alternatively, the data for JKT-1, together with experiments from us and others, further indicate that JKT-1 does not (or no longer) display seminoma-like characteristics. 25,26 To address the question whether NANOG expression correlates to methylation of the CpGs in the NRR in GCTs and derived cell lines, we performed qRT-PCR analysis. NANOG expression levels were high in seminomas and embryonal carcinomas, while low and absent in teratomas, yolk sac tumors, choriocarcinomas and mixed non-seminomas (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…It is postulated that seminomas are not able to differentiate in vivo (Battifora et al, 1984;Srigley et al, 1988), although some rare cases of seminomatous in vivo differentiation were described (Czaja and Ulbright, 1992) and a transition step to ECs is hypothesized (Oosterhuis and Looijenga, 2005 #23). The TCam-2 cell line resembles a seminoma since it expresses markers of primordial germ cells (PGC) and seminomas as well as markers indicative for pluripotency and self-renewal (KIT, TFAP2C, BLIMP1, VASA, GDF3, MCFD2, D2-40, SOX17, NANOG, OCT3/4) (de Jong et al, 2008a;Eckert et al, 2008). Alike seminomas, TCam-2 cells lack expression of SOX2 but are positive for SOX17 (de Jong et al, 2008b).…”
Section: Introductionmentioning
confidence: 98%
“…20 In this study, we focused on SE using the TCAM2 cell lines containing typical features of human SE. 21 We aimed to investigate a functional link between the ERβ and PTEN and evidenced that E2 induced PTEN expression, defining the molecular mechanism. We propose that PTEN might be one of the signaling proteins on which E2 is acting to affect seminoma cell survival.…”
Section: Introductionmentioning
confidence: 99%