TCF-based fluorescent probe for monitoring superoxide anion produced in bacteria under chloramphenicol- and heat-induced stress

Abstract

We report on a superoxide anion (O2˙−) responsive fluorescent probe called TCF-OTf.

Visual monitoring of biocatalytic processes using small molecular fluorescent probes: strategies-mechanisms-applications

“…[67][68][69][70] Hypoxic conditions cause reductases such as nitroreductase (NTR) and azoreductase (AzoR) to be overexpressed in cancer cells. [71][72][73][74][75][76] Thus, the visual monitoring of biocatalytic nitro and azoreductions are important for cancer diagnosis and treatment.…”

A Sequentially Activated Probe for Imaging of Superoxide Anion and Peroxynitrite in PC12 Cells under Oxidative Stress

“…Given the high reactivity and short lifespan of O 2 ·– and ONOO – , conventional histological and biochemical analysis techniques lack the ability to assess their dynamic changes within cells or in vivo in real-time. Fluorescence imaging offers a crucial tool for the noninvasive investigation of biological processes in cells and transparent organisms due to its superior spatiotemporal resolution, sensitivity, and ability to detect targets in situ and/or in real-time within complex biosystems − While numerous fluorescent probes have been developed for the specific detection of O 2 ·– − or ONOO – , − few have been able to simultaneously visualize both species in vitro and in vivo . Although it is possible to use a single fluorescent probe for O 2 ·– and another for ONOO – simultaneously, data interpretation becomes challenging due to their different uptake, distribution, and metabolism profiles.…”