Tcf12 is required to sustain myogenic genes synergism with MyoD by remodelling the chromatin landscape

Wang, Sheng; Liao, Yinlong; Zhang, Haoyuan; Jiang, Yuxin; Peng, Zhelun; Ren, Ruimin; Li, Xinyun; Wang, Heng

doi:10.1038/s42003-022-04176-0

Cited by 11 publications

(9 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed that rs4361970 was most accessible in E8.5 spinal cord cells ( Figure 4D ). We also found rs2733330 region at Tcf12 locus ( Cao et al, 2010 ; Ke et al, 2021 ; Wang et al, 2022 ). Tcf12 bind to the E-box regions of Myod and Myog, contributing to SMP ( Parker et al, 2006 ).…”

Section: Resultssupporting

confidence: 62%

Single-cell chromatin accessibility profiling of cell-state-specific gene regulatory programs during mouse organogenesis

et al. 2023

View full text Add to dashboard Cite

In mammals, early organogenesis begins soon after gastrulation, accompanied by specification of various type of progenitor/precusor cells. In order to reveal dynamic chromatin landscape of precursor cells and decipher the underlying molecular mechanism driving early mouse organogenesis, we performed single-cell ATAC-seq of E8.5-E10.5 mouse embryos. We profiled a total of 101,599 single cells and identified 41 specific cell types at these stages. Besides, by performing integrated analysis of scATAC-seq and public scRNA-seq data, we identified the critical cis-regulatory elements and key transcription factors which drving development of spinal cord and somitogenesis. Furthermore, we intersected accessible peaks with human diseases/traits-related loci and found potential clinical associated single nucleotide variants (SNPs). Overall, our work provides a fundamental source for understanding cell fate determination and revealing the underlying mechanism during postimplantation embryonic development, and expand our knowledge of pathology for human developmental malformations.

show abstract

Section: Resultssupporting

confidence: 62%

Single-cell chromatin accessibility profiling of cell-state-specific gene regulatory programs during mouse organogenesis

et al. 2023

View full text Add to dashboard Cite

show abstract

“…For example, highly activated CX muscle enhancers were enriched with the TFs MEF2D , MEF2A , MEF2C , FOSL2 and BACH2 , which showed the potential target genes like UTRN , NEB , SGCE , TCF12 and MKX . Among these potentially targeted UTRN and NEB genes associated with certain muscle‐related diseases (Deconinck et al., 1997; Ranu et al., 2022), TCF12 was reported to regulate muscle development (Wang et al., 2022). In addition, highly activated CX adipose enhancers were enriched with the TFs FOSL2 , ATF3 , JUNB , SIX2 and MEF2D .…”

Section: Discussionmentioning

confidence: 99%

“…Among these genes, PPARG, LPIN1 and CEBPB could influence adipocyte differentiation, lipid metabolism and glucose homeostasis in adipose tissue (Barroso et al, 1999;Park et al, 2010;Reue & Zhang, 2008). TCF12 and PPARG have already been reported as key genes related to muscle development and fatty acid formation (Barroso et al, 1999;Wang et al, 2022). Combining the important role of TFs enriched in the highly activated CX muscle enhancers or highly activated CX fat enhancers and their potential target genes, the muscle and fat tissue function associated regulatory network revealed by this study can provide theoretical support for further study of pork quality.…”

Section: Discussionmentioning

confidence: 99%

H3K27ac modification and transcription characteristics of adipose and muscle tissues in Chuxiang Black pig

Kuang,

Xu,

Zhou

et al. 2024

Animal Genetics

View full text Add to dashboard Cite

The establishment of high‐quality pork breeds for improving meat quality in the pig industry is needed. The Chuxiang Black (CX) pig is a new breed developed from Chinese local pigs and Western lean pigs that has a high proportion of lean meat and excellent meat quality. However, the characteristics of cis‐regulatory elements in CX pigs are still unknown. In this study, cis‐regulatory elements of muscle and adipose tissues in CX pigs were investigated using ChIP‐seq and RNA sequencing. Compared with the reported cis‐regulatory elements of muscle and adipose tissues, 1768 and 1012 highly activated enhancers and 433 and 275 highly activated promoters in CX muscle and adipose tissues were identified, respectively. Motif analysis showed that transcription factors, such as MEF2A and MEF2C, were core regulators of highly activated enhancers and promoters in muscle. Similarly, the transcription factors JUNB and CUX1 were identified as essential for highly activated enhancers and promoters in CX adipose tissue. These results enrich the resources for the analysis of cis‐regulatory elements in the pig genome and provide new basic data for further meat quality improvement through breeding in pigs.

show abstract

“…Additionally, the amyloid beta precursor protein binding family A member 3 ( APBA3 ) gene, situated in the region from 21.44 Mb to 21.45 Mb, has been linked to ovarian hyperstimulation for in vitro fertilization in women [ 78 ]. Furthermore, the transcription factor 12 ( TCF12 ) gene, found between 53.05 Mb and 53.52 Mb, has been observed to play a role in muscle development and regeneration, as indicated by loss-of-function studies, and is important in regulating cell growth and differentiation during embryonic development [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study identifies genomic regions associated with key reproductive traits in Korean Hanwoo cows

Haque,

Lee,

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background Conducting genome-wide association studies (GWAS) for reproductive traits in Hanwoo cattle, including age at first calving (AFC), calving interval (CI), gestation length (GL), and number of artificial inseminations per conception (NAIPC), is of paramount significance. These analyses provided a thorough exploration of the genetic basis of these traits, facilitating the identification of key markers for targeted trait improvement. Breeders can optimize their selection strategies, leading to more efficient and sustainable breeding programs, by incorporating genetic insights. This impact extends beyond individual traits and contributes to the overall productivity and profitability of the Hanwoo beef cattle industry. Ultimately, GWAS is essential in ensuring the long-term genetic resilience and adaptability of Hanwoo cattle populations. The primary goal of this study was to identify significant single nucleotide polymorphisms (SNPs) or quantitative trait loci (QTLs) associated with the studied reproductive traits and subsequently map the underlying genes that hold promise for trait improvement. Results A genome-wide association study of reproductive traits identified 68 significant single nucleotide polymorphisms (SNPs) distributed across 29 Bos taurus autosomes (BTA). Among them, BTA14 exhibited the highest number of identified SNPs (25), whereas BTA6, BTA7, BTA8, BTA10, BTA13, BTA17, and BTA20 exhibited 8, 5, 5, 3, 8, 2, and 12 significant SNPs, respectively. Annotation of candidate genes within a 500 kb region surrounding the significant SNPs led to the identification of ten candidate genes relevant to age at first calving. These genes were: FANCG, UNC13B, TESK1, TLN1, and CREB3 on BTA8; FAM110B, UBXN2B, SDCBP, and TOX on BTA14; and MAP3K1 on BTA20. Additionally, APBA3, TCF12, and ZFR2, located on BTA7 and BTA10, were associated with the calving interval; PAX1, SGCD, and HAND1, located on BTA7 and BTA13, were linked to gestation length; and RBM47, UBE2K, and GPX8, located on BTA6 and BTA20, were linked to the number of artificial inseminations per conception in Hanwoo cows. Conclusions The findings of this study enhance our knowledge of the genetic factors that influence reproductive traits in Hanwoo cattle populations and provide a foundation for future breeding strategies focused on improving desirable traits in beef cattle. This research offers new evidence and insights into the genetic variants and genome regions associated with reproductive traits and contributes valuable information to guide future efforts in cattle breeding.

show abstract

Tcf12 is required to sustain myogenic genes synergism with MyoD by remodelling the chromatin landscape

Cited by 11 publications

References 70 publications

Single-cell chromatin accessibility profiling of cell-state-specific gene regulatory programs during mouse organogenesis

Single-cell chromatin accessibility profiling of cell-state-specific gene regulatory programs during mouse organogenesis

H3K27ac modification and transcription characteristics of adipose and muscle tissues in Chuxiang Black pig

Genome-wide association study identifies genomic regions associated with key reproductive traits in Korean Hanwoo cows

Contact Info

Product

Resources

About