TCF19 enhances cell proliferation in hepatocellular carcinoma by activating the ATK/FOXO1 signaling pathway

Zeng, Chenglong; Feng, Wenhua; Zhao, Junfei; Li, F. X.; Gao, Peng

doi:10.4149/neo_2018_171227n845

Cited by 20 publications

(23 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…37 Subsequently, we demonstrated that miR-1207-5p directly inhibited the expression of TCF19, a newly identified target of miR-1207-5p, that could play an oncogenic role in several cancers, such as colorectal cancer, 23 non-small cell lung cancer, 24 and liver cancer. 22,38 Consistent with the findings of previous studies, 22,38 we demonstrated that TCF19 promoted cell proliferation in HCC. Besides, we further showed the migratory capacities of liver cancer cells regulated by TCF19.…”

Section: Discussionsupporting

confidence: 92%

“…[22][23][24] Herein, we also demonstrated that TCF19 promoted cell proliferation in liver cancer by MTT assay and colony formation assay ( Figure 6A-C), which was consistent with the demonstrations in the previous report. 22 However, whether TCF19 also affected the migration and invasion abilities of liver cancer cells was unreported. Therefore, we measured the migration and invasion of liver cancer cells by altering the expression of TCF19.…”

Section: Tcf19 Promoted the Proliferation And Metastasis Of Liver Cansupporting

confidence: 91%

“…Accordingly, the TCF19 mRNA level was upregulated in HCC tissues ( Figure 5E), which was consistent with the demonstrations in the previous report. 22 We also observed that the expression of miR-1207-5p was inversely related to the TCF19 mRNA level ( Figure 5F). These results suggest that TCF19 is a direct target of miR-1207-5p, and there is a high probability that it is positively regulated by MIR194-2HG.…”

Section: Tcf19 Was a Direct Target Of Mir-1207-5pmentioning

confidence: 52%

See 2 more Smart Citations

<p>LncRNA MIR194-2HG Promotes Cell Proliferation and Metastasis via Regulation of miR-1207-5p/TCF19/Wnt/β-Catenin Signaling in Liver Cancer</p>

Xu¹,

Zhu²,

Liu³

et al. 2020

OTT

View full text Add to dashboard Cite

LncRNAs play an important role in tumorigenesis and cancer progression in liver cancer. Although many lncRNAs have been reported, the role of MIR194-2HG and the underlying mechanism mediated by it are still largely unknown in HCC. This study aimed to investigate the biological role and mechanism of MIR194-2HG in liver cancer. Materials and Methods: The expression of MIR194-2HG was determined in liver cancer tissues and cells by RT-qPCR. The overall survival rate of MIR194-2HG was analyzed by Kaplan-Meier survival analysis. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, and Transwell assays were carried out to detect cell migration and invasion. Western blotting was used to quantify the levels of all proteins. The regulatory mechanism of the MIR194-2HG/miR-1207-5p/TCF19 axis in liver cancer was investigated by dual-luciferase activity reporter assay, Kaplan-Meier survival analysis, and Western blotting. Results: MIR194-2HG was upregulated in liver cancer tissues and cell lines. Liver cancer patients with higher expression of MIR194-2HG revealed poor overall survival compared with those who had lower expression of MIR194-2HG. MIR194-2HG promoted the proliferation, migration, and invasion of HepG2 and Huh7 cells by acting as a ceRNA mechanism for the miR-1207-5p/TCF19 axis to activate the Wnt/β-catenin signaling pathway. Conclusion: MIR194-2HG acts in an oncogenic role and activates the Wnt/β-catenin signaling pathway via a miR-1207-5p/TCF19 axis-mediated mechanism, which provides a novel avenue for diagnostic or therapeutic interventions in liver cancer.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Tcf19 Promoted the Proliferation And Metastasis Of Liver Cansupporting

confidence: 91%

Section: Tcf19 Was a Direct Target Of Mir-1207-5pmentioning

confidence: 52%

See 1 more Smart Citation

<p>LncRNA MIR194-2HG Promotes Cell Proliferation and Metastasis via Regulation of miR-1207-5p/TCF19/Wnt/β-Catenin Signaling in Liver Cancer</p>

Xu¹,

Zhu²,

Liu³

et al. 2020

OTT

View full text Add to dashboard Cite

show abstract

“…There were no reports of the expression level of THBD in brain tumors, our study showed that THBD was highly expressed in GBM patients, and patients with different expression levels had inequable prognosis. Finally, several studies had found that in non-small cell lung cancer, hepatocellular carcinoma, and colorectal cancer TCF19 (transcription factor 19) could promote cell proliferation and increase tumor invasiveness through some pathways, but there were no reports about its role in GBM [33][34][35][36]. Our study found that TCF19 was highly expressed in GBM patients, and patients with different expression levels also had inequable prognosis.…”

Section: Discussionmentioning

confidence: 54%

Identification of novel genes associated with diagnosis and prognosis in glioblastoma

Cai

Tang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Glioblastoma (GBM) is the most lethal primary brain cancer and its survival rate is very low. Comprehensive genomic characteristics and high degree of heterogeneity of GBM are main causes that contribute to the absence of effective therapeutic targets and prognostic markers and eventual treatment failures. Here, Gene set enrichment analysis (GSEA) was used to explore comprehensive genomic characteristics and high degree of heterogeneity of GBM. Our study will help explain potential tumorigenesis mechanism and contribute to development of targeted therapeutics and prediction of patient prognosis.Methods: Gene expression profile of GSE50161 was downloaded from Gene Expression Omnibus (GEO) database and GSEA was performed to evaluate the microarray data at level of gene sets of GO, KEGG and hallmark of Molecular Signatures Database (MSigDB). Core enrichments of selected hallmark gene sets were applied for clinical prognosis verification in Gene Expression Profiling Interactive Analysis (GEPIA). Genes associated with significant overall survival (OS) and unreported before were recognized as novel; the expression levels of novel genes in GBM samples and every novel gene between normal brain and GBM samples were compared. Receiver operating characteristic (ROC) and Pearson correlation analysis were also performed to evaluate the diagnostic biomarkers of GBM and correlations among novel genes respectively.Results: We obtained 511 and 494 GO gene sets, 12 and 10 KEGG gene sets, and 2 and 8 hallmark gene sets in normal and GBM samples respectively. Five novel genes SERPINA5, TENM2, ARAP3, THBD, TCF19 associated with GBM prognosis were selected and four novel genes SERPINA5, TENM2, ARAP3, TCF19 performed well for GBM diagnosis prediction were obtained. In addition, we also found that four novel genes SERPINA5, ARAP3, THBD, TCF19 that high expressed in GBM samples were all positively correlated with each other and correlation between ARAP3 and TCF19 was the strongest.Conclusions: Our study will help deeper understand the molecular heterogeneity and develop of targeted therapeutics of GBM; five novel related to prognosis genes (of which four are also related to diagnosis) may be applied to more accurate clinical decision-making process.

show abstract

“…SNP in tumor suppressor RAD54L is important for the development of pancreatic cancer [176], but this polymorphic gene may be identified with the development of BRCA. Genes, such as CDCA3 [177], KIF15 [178], and TCF19 [179], are linked with the proliferation of various cancer cells, such as oral cancer, pancreatic cancer, and hepatocellular carcinoma cells, but these genes may be responsible for the proliferation of BRCA cells. Genes, such as BOP1 [180], KIF11 [181], and MMS22L [182], are associated with the progression of various cancers, such as colorectal cancer, gastric cancer, lung, and esophageal cancer, but these genes may be linked with the development of BRCA.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Molecular Mechanism of the Drug-Treated Breast Cancer Based on Gene Expression Microarray

Alshabi

Vastrad²,

Shaikh

et al. 2019

Biomolecules

View full text Add to dashboard Cite

: Breast cancer (BRCA) remains the leading cause of cancer morbidity and mortality worldwide. In the present study, we identified novel biomarkers expressed during estradiol and tamoxifen treatment of BRCA. The microarray dataset of E-MTAB-4975 from Array Express database was downloaded, and the differential expressed genes (DEGs) between estradiol-treated BRCA sample and tamoxifen-treated BRCA sample were identified by limma package. The pathway and gene ontology (GO) enrichment analysis, construction of protein-protein interaction (PPI) network, module analysis, construction of target genes—miRNA interaction network and target genes-transcription factor (TF) interaction network were performed using bioinformatics tools. The expression, prognostic values, and mutation of hub genes were validated by SurvExpress database, cBioPortal, and human protein atlas (HPA) database. A total of 856 genes (421 up-regulated genes and 435 down-regulated genes) were identified in T47D (overexpressing Split Ends (SPEN) + estradiol) samples compared to T47D (overexpressing Split Ends (SPEN) + tamoxifen) samples. Pathway and GO enrichment analysis revealed that the DEGs were mainly enriched in response to lysine degradation II (pipecolate pathway), cholesterol biosynthesis pathway, cell cycle pathway, and response to cytokine pathway. DEGs (MCM2, TCF4, OLR1, HSPA5, MAP1LC3B, SQSTM1, NEU1, HIST1H1B, RAD51, RFC3, MCM10, ISG15, TNFRSF10B, GBP2, IGFBP5, SOD2, DHF and MT1H) , which were significantly up- and down-regulated in estradiol and tamoxifen-treated BRCA samples, were selected as hub genes according to the results of protein-protein interaction (PPI) network, module analysis, target genes—miRNA interaction network and target genes-TF interaction network analysis. The SurvExpress database, cBioPortal, and Human Protein Atlas (HPA) database further confirmed that patients with higher expression levels of these hub genes experienced a shorter overall survival. A comprehensive bioinformatics analysis was performed, and potential therapeutic applications of estradiol and tamoxifen were predicted in BRCA samples. The data may unravel the future molecular mechanisms of BRCA.

show abstract

TCF19 enhances cell proliferation in hepatocellular carcinoma by activating the ATK/FOXO1 signaling pathway

Cited by 20 publications

References 25 publications

<p>LncRNA MIR194-2HG Promotes Cell Proliferation and Metastasis via Regulation of miR-1207-5p/TCF19/Wnt/β-Catenin Signaling in Liver Cancer</p>

<p>LncRNA MIR194-2HG Promotes Cell Proliferation and Metastasis via Regulation of miR-1207-5p/TCF19/Wnt/β-Catenin Signaling in Liver Cancer</p>

Identification of novel genes associated with diagnosis and prognosis in glioblastoma

Exploring the Molecular Mechanism of the Drug-Treated Breast Cancer Based on Gene Expression Microarray

Contact Info

Product

Resources

About