TcMYB8, a R3-MYB Transcription Factor, Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

Zhou, Li; Li, Jiawen; Zeng, Tuo; Xu, Zhizhuo; Jia, Luo; Zheng, Rencheng; Wang, Yuanyuan; Wang, Caiyun

doi:10.3390/ijms232012186

Cited by 6 publications

(5 citation statements)

References 53 publications

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“…Accordingly, EPS not only stimulates the expression of ABA and GA key enzyme genes ( GA2ox and CYP707A ) in S. baicalensis calli but also promotes the expression of MYB3 , MYB8 , and MYB13 . Based on our experimental results and relevant literature ( Bang et al., 2008 ; Yuan et al., 2013 , 2015 ; Qian et al., 2020 ; Zhou et al., 2022 ; Fang et al., 2023 ; He et al., 2023 ), EPS treatment induces flavonoid synthesis in S. baicalensis calli by regulating the expression of phytohormones, TFs, and enzymes. That is, EPS might stimulate the expression of genes encoding MYB3, MYB8, MYB13, GA2ox, and CYP707A, leading to significantly higher expression of CHS , CHI , FNS , and F6H and ultimately promoting the synthesis of flavonoids.…”

Section: Discussionmentioning

confidence: 72%

“…For example, Yuan et al (2013) showed that GA metabolism contributes to flavonoid biosynthesis, with the expression of MYB8 significantly improving in S. baicalensis within 3 h of GA treatment. Furthermore, ABA can improve the expression of MYB3 and MYB8 in a relatively short period of time (Bang et al, 2008;Zhou et al, 2022). Accordingly, EPS not only stimulates the expression of ABA and GA key enzyme genes (GA2ox and CYP707A) in S. baicalensis calli but also promotes the expression of MYB3, MYB8, and MYB13.…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, TFs, such as the NAC and MYB families, are key regulators of flavonoid synthesis ( Yuan et al., 2013 , 2015 ; Qian et al., 2020 ; Fang et al., 2023 , 2023 ; He et al., 2023 ). The crosstalk between plant hormones and TFs adds another layer of regulation to secondary metabolite formation and accumulation ( Bang et al., 2008 ; Yuan et al., 2013 ; Zhou et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Cui,

Zhang,

Sun

et al. 2024

Front. Plant Sci.

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IntroductionEndophytic fungi can promote secondary metabolite accumulation in medicinal plants. Previously, we observed that the culture filtrate of Fusarium solani CL105 promoted flavonoid production in Scutellaria baicalensis calli. However, the active ingredients and mechanisms associated with this secondary metabolite accumulation remain unclear.MethodsThis study evaluates the effects of different elicitors from the culture filtrate of F. solani CL105 namely, exopolysaccharide (EPS), exoprotein (EP), and other parts (OP), on the flavonoid production in S. baicalensis calli by HPLC. Subsequently, the underlying mechanism of EPS induced flavonoid production in S. baicalensis calli was revealed by transcriptomics and RT-PCR.Results and discussionThe results indicated a significant increase in flavonoid production in S. baicalensis calli following treatment with EPS. Baicalin (1.40 fold), wogonoside (1.91 fold), and wogonin (2.76 fold) were most significantly up-regulated compared with the control. Transcriptome analysis further revealed up-regulation of key enzyme genes (CHS, CHI, FNS, and F6H) involved in flavonoid synthesis after 5 days of EPS treatment. Moreover, the expression of GA2ox and CYP707A—genes involved in gibberellin acid (GA) and abscisic acid biosynthesis (ABA), respectively—were significantly up-regulated. The expression levels of certain transcription factors, including MYB3, MYB8, and MYB13, were also significantly higher than in controls. Our results indicated that EPS was a main active elicitor involved in promoting flavonoid production in S. baicalensis calli. We postulated that EPS might stimulate the expression of MYB3, MYB8, MYB13, GA2ox, and CYP707A, leading to markedly upregulated CHS, CHI, FNS, and F6H expression levels, ultimately promoting flavonoid synthesis. This study provides a novel avenue for large-scale in vitro production of flavonoids in S. baicalensis.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Cui,

Zhang,

Sun

et al. 2024

Front. Plant Sci.

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“…SiMYB75 positively regulates the responses to drought, salt, and osmotic stress through an ABA-mediated pathway [83]. TcMYB8 and SbMYB12 can respond to MeJA treatment [84,85], and GlMYB4 and GlMYB88 play important roles in the synthesis of flavonoids mediated using MeJA signaling [86]. In this study, we found that regulatory elements associated with MeJA and ABA were most common in EfMYB; thus, we also analyzed the expression characteristics of the nine EfMYB genes after ABA and MeJA treatments.…”

Section: Discussionmentioning

confidence: 88%

Genome-Wide Identification and Expression Pattern of MYB Family Transcription Factors in Erianthus fulvus

Rao,

Qian,

Xie

et al. 2023

Genes

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MYB family genes have many functions and are widely involved in plant abiotic-stress responses. Erianthus fulvus is an important donor material for stress-resistance genes in sugarcane breeding. However, the MYB family genes in E. fulvus have not been systematically investigated. In this study, 133 EfMYB genes, including 48 Ef1R-MYB, 84 EfR2R3-MYB and 1 Ef3R-MYB genes, were identified in the E. fulvus genome. Among them, the EfR2R3-MYB genes were classified into 20 subgroups. In addition, these EfMYB genes were unevenly distributed across 10 chromosomes. A total of 4 pairs of tandemly duplicated EfMYB genes and 21 pairs of segmentally duplicated EfMYB genes were identified in the E. fulvus genome. Protein-interaction analysis predicted that 24 EfMYB proteins had potential interactions with 14 other family proteins. The EfMYB promoter mainly contains cis-acting elements related to the hormone response, stress response, and light response. Expression analysis showed that EfMYB39, EfMYB84, and EfMYB124 could be significantly induced using low-temperature stress. EfMYB30, EfMYB70, EfMYB81, and EfMYB101 responded positively to drought stress. ABA treatment significantly induced EfMYB1, EfMYB30, EfMYB39, EfMYB84, and EfMYB130. All nine genes were induced using MeJA treatment. These results provide comprehensive information on EfMYB genes and can serve as a reference for further studies of gene function.

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“…The expression vector pGreenII62-SK was driven by the CaMV35S promoter and the constructed pGreenII62-SK: TcbHLH14 vector was transformed into A. tumefaciens GV3101 (containing the pSoup19 vector). Transient transformation of T. cinerariifolium leaves was performed as described previously [ 46 ]. After the RT-qPCR assay, 4-day-old leaves were taken for HPLC analysis.…”

Section: Methodsmentioning

confidence: 99%

TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

Zeng

Junzhong

et al. 2023

IJMS

Self Cite

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Natural pyrethrins have high application value, and are widely used as a green pesticide in crop pest prevention and control. Pyrethrins are mainly extracted from the flower heads of Tanacetum cinerariifolium; however, the natural content is low. Therefore, it is essential to understand the regulatory mechanisms underlying the synthesis of pyrethrins through identification of key transcription factors. We identified a gene encoding a MYC2-like transcription factor named TcbHLH14 from T. cinerariifolium transcriptome, which is induced by methyl jasmonate. In the present study, we evaluated the regulatory effects and mechanisms of TcbHLH14 using expression analysis, a yeast one-hybrid assay, electrophoretic mobility shift assay, and overexpression/virus-induced gene silencing experiments. We found that TcbHLH14 can directly bind to the cis-elements of the pyrethrins synthesis genes TcAOC and TcGLIP to activate their expression. The transient overexpression of TcbHLH14 enhanced expression of the TcAOC and TcGLIP genes. Conversely, transient silencing of TcbHLH14 downregulated the expression of TcAOC and TcGLIP and reduced the content of pyrethrins. In summary, these results indicate that the potential application of TcbHLH14 in improving the germplasm resources and provide a new insight into the regulatory network of pyrethrins biosynthesis of T. cinerariifolium to further inform the development of engineering strategies for increasing pyrethrins contents.

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TcMYB8, a R3-MYB Transcription Factor, Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

Cited by 6 publications

References 53 publications

Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Genome-Wide Identification and Expression Pattern of MYB Family Transcription Factors in Erianthus fulvus

TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

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