TCPs, WUSs, and WINDs: families of transcription factors that regulate shoot meristem formation, stem cell maintenance, and somatic cell differentiation

Ikeda, Miho; Ohme‐Takagi, Masaru

doi:10.3389/fpls.2014.00427

Cited by 25 publications

(22 citation statements)

References 37 publications

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“…The first pattern (Figure S9A – Additional file 1 ) is composed by the transcriptional factor TCP5 (SP803280_c109776_g3) that monotonically increased its expression from B0 to T segments. Members of this family are involved with leaf morphogenesis and differentiation [ 68 – 70 ], arrest of cell division [ 71 ], leaf elongation [ 72 ] and auxin response [ 73 ]. To date, there is no information on the role of TCP5 in C 4 plants and its expression profile in our dataset suggests that this gene can be an important player during leaf development.…”

Section: Resultsmentioning

confidence: 99%

Physiological and transcriptional analyses of developmental stages along sugarcane leaf

et al. 2015

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BackgroundSugarcane is one of the major crops worldwide. It is cultivated in over 100 countries on 22 million ha. The complex genetic architecture and the lack of a complete genomic sequence in sugarcane hamper the adoption of molecular approaches to study its physiology and to develop new varieties. Investments on the development of new sugarcane varieties have been made to maximize sucrose yield, a trait dependent on photosynthetic capacity. However, detailed studies on sugarcane leaves are scarce. In this work, we report the first molecular and physiological characterization of events taking place along a leaf developmental gradient in sugarcane.ResultsPhotosynthetic response to CO2 indicated divergence in photosynthetic capacity based on PEPcase activity, corroborated by activity quantification (both in vivo and in vitro) and distinct levels of carbon discrimination on different segments along leaf length. Additionally, leaf segments had contrasting amount of chlorophyll, nitrogen and sugars. RNA-Seq data indicated a plethora of biochemical pathways differentially expressed along the leaf. Some transcription factors families were enriched on each segment and their putative functions corroborate with the distinct developmental stages. Several genes with higher expression in the middle segment, the one with the highest photosynthetic rates, were identified and their role in sugarcane productivity is discussed. Interestingly, sugarcane leaf segments had a different transcriptional behavior compared to previously published data from maize.ConclusionThis is the first report of leaf developmental analysis in sugarcane. Our data on sugarcane is another source of information for further studies aiming to understand and/or improve C4 photosynthesis. The segments used in this work were distinct in their physiological status allowing deeper molecular analysis. Although limited in some aspects, the comparison to maize indicates that all data acquired on one C4 species cannot always be easily extrapolated to other species. However, our data indicates that some transcriptional factors were segment-specific and the sugarcane leaf undergoes through the process of suberizarion, photosynthesis establishment and senescence.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0694-z) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Physiological and transcriptional analyses of developmental stages along sugarcane leaf

et al. 2015

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“…Phytoplasmas are bacterial pathogens that depend on phloem-feeding leafhoppers for transmission. The Aster Yellows phytoplasma strain Witches’ Broom (AY-WB) produces a Sec-secreted effector called SAP11, which destabilizes the TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL FACTORS (TCP) class of transcription factors in Arabidopsis (Ikeda and Ohme-Takagi 2014 ). Among them, TCP4 activates the expression of JA biosynthetic gene LIPOXYGENASE 2 ( LOX2 ) (Schommer et al 2008 ).…”

Section: Plant Hormones As Key Regulators Of Immunitymentioning

confidence: 99%

Phytohormone pathways as targets of pathogens to facilitate infection

2016

Plant Mol Biol

149

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Plants are constantly threatened by potential pathogens. In order to optimize the output of defense against pathogens with distinct lifestyles, plants depend on hormonal networks to fine-tune specific responses and regulate growth-defense tradeoffs. To counteract, pathogens have evolved various strategies to disturb hormonal homeostasis and facilitate infection. Many pathogens synthesize plant hormones; more importantly, toxins and effectors are produced to manipulate hormonal crosstalk. Accumulating evidence has shown that pathogens exert extensive effects on plant hormone pathways not only to defeat immunity, but also modify habitat structure, optimize nutrient acquisition, and facilitate pathogen dissemination. In this review, we summarize mechanisms by which a wide array of pathogens gain benefits from manipulating plant hormone pathways.

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“…Wounding was shown to promote regeneration-competent callus development from Arabidopsis roots in a WIND1 transcription factor-dependent way (Iwase et al 2011a, b;Ikeuchi et al 2016). Endogenous auxin level is not significantly changed in wounded Arabidopsis hypocotyls (Iwase et al 2017) and WIND1 has no effect on endogenous auxin level (Ikeda and Ohme-Takagi 2014;Iwase et al 2017) but was shown to activate cytokinin synthesis and responses (Iwase et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Timely removal of exogenous cytokinin and the prevention of auxin transport from the shoot to the root affect the regeneration potential of Arabidopsis roots

Bernula

Benkő

Kaszler

et al. 2019

Plant Cell Tiss Organ Cult

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In vitro regeneration of Arabidopsis from roots is generally achieved via indirect organogenesis. First, transdifferentiation of lateral root primordia to calli is achieved by a balanced auxin-to-cytokinin ratio that is followed by the induction of shoot meristem formation using a high cytokinin level. Here we demonstrate that if the root explants were transferred onto a hormone-free medium after a transient (4-days) cytokinin treatment, embryogenic marker genes (LEC1, LEC2, FUS3) started to be expressed. App. 50% of the regeneration foci developed into plantlets with trichome-less cotyledon-like leaves. Moreover, the somatic embryogenesis defective lec1 mutant could regenerate only shoots with trichome-bearing leaves under this condition. Based on these observations, the mixed accomplishment of shoot organogenesis and somatic embryogenesis is hypothesized in the Arabidopsis root explants cultured under hormone-free conditions following cytokinin induction. Using whole seedlings instead of root explants in the same experimental set up, no regenerates were formed on the roots. Applying the auxin transport inhibitor TIBA to the root-to-shoot junction of the seedlings, the regeneration ability of the root could be restored. The observations indicate that shoot-derived endogenous auxin blocks the cytokinin-induced regeneration process in the roots of whole seedlings. The expression of the wound-induced transcription factor WIND1 could be detected in the roots of unwounded seedlings if the shoot-to-root auxin transport was inhibited. Manipulating the exogenous cytokinin level together with the endogenous shoot-to-root auxin transport therefore could mimic the effect of wounding (removal of shoot) on plant regeneration from roots. Key message Transferring root explants from high cytokinin to hormone-free conditions resulted in the expression of embryogenic markers. Inhibiting the shoot-to-root auxin transport had similar effect on regeneration as wounding.

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TCPs, WUSs, and WINDs: families of transcription factors that regulate shoot meristem formation, stem cell maintenance, and somatic cell differentiation

Cited by 25 publications

References 37 publications

Physiological and transcriptional analyses of developmental stages along sugarcane leaf

Physiological and transcriptional analyses of developmental stages along sugarcane leaf

Phytohormone pathways as targets of pathogens to facilitate infection

Timely removal of exogenous cytokinin and the prevention of auxin transport from the shoot to the root affect the regeneration potential of Arabidopsis roots

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