TDM1 Regulation Determines the Number of Meiotic Divisions

Cifuentes, Marta; Jolivet, Sylvie; Cromer, Laurence; Harashima, Hirofumi; Bulánková, Petra; Rolny, Charlotte; Crismani, Wayne; Nomura, Yuko; Nakagami, Hirofumi; Sugimoto, Keiko; Schnittger, Arp; Říha, Karel; Mercier, Raphaël

doi:10.1371/journal.pgen.1005856

Cited by 47 publications

(77 citation statements)

References 45 publications

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“…). Previous studies demonstrated that OSD1 is an APC/C inhibitor, while TDM1 is present throughout meiosis and its activity must be inhibited during meiosis I (Cromer et al ., ; Cifuentes et al ., ). Our qRT‐PCR data show that the expression of both genes is elevated in apc8‐1 compared with wild‐type, indicating that there may be a feedback mechanism between APC/C and its regulators.…”

Section: Discussionmentioning

confidence: 97%

“…The essential function of APC/C subunits in plants has limited our understanding of their function during chromosome segregation. Evidence suggests that Arabidopsis TDM1 is an APC/C component and is required for the termination of meiosis II (Cromer et al, 2012;Cifuentes et al, 2016). OSD1 promotes entry into the second meiotic division, likely to be through inhibition of APC/C activity (Cromer et al, 2012).…”

Section: Conservation and Divergence Of Apc/c Activity In Meiosis Amomentioning

confidence: 99%

“…Although meiotic functions of the known plant APC/C subunits have not been reported, several meiotic regulators associated with APC/C have been identified. THREE DIVISION MUTANT1/MALE STERILE 5 (TDM1/MS5) regulates meiotic exit and is a putative meiotic APC/C component based on its shared structural similarities with the APC/C subunit CDC16/Cut9/APC6 (Cromer et al, 2012;Cifuentes et al, 2016). The direct interaction between TDM1with the APC/C activator CDC20.1 and the APC/C core component CDC27b (HOBBIT) further supports the hypothesis that TDM1 acts via the activation of the APC/C and/or by modifying its specificity.…”

Section: Introductionmentioning

confidence: 99%

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The Arabidopsis anaphase‐promoting complex/cyclosome subunit 8 is required for male meiosis

Wang

et al. 2019

New Phytologist

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Summary Faithful chromosome segregation is required for both mitotic and meiotic cell divisions and is regulated by multiple mechanisms including the anaphase‐promoting complex/cyclosome (APC/C), which is the largest known E3 ubiquitin‐ligase complex and has been implicated in regulating chromosome segregation in both mitosis and meiosis in animals. However, the role of the APC/C during plant meiosis remains largely unknown. Here, we show that Arabidopsis APC8 is required for male meiosis. We used a combination of genetic analyses, cytology and immunolocalisation to define the function of AtAPC8 in male meiosis. Meiocytes from apc8‐1 plants exhibit several meiotic defects including improper alignment of bivalents at metaphase I, unequal chromosome segregation during anaphase II, and subsequent formation of polyads. Immunolocalisation using an antitubulin antibody showed that APC8 is required for normal spindle morphology. We also observed mitotic defects in apc8‐1, including abnormal sister chromatid segregation and microtubule morphology. Our results demonstrate that Arabidopsis APC/C is required for meiotic chromosome segregation and that APC/C‐mediated regulation of meiotic chromosome segregation is a conserved mechanism among eukaryotes.

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Section: Discussionmentioning

confidence: 97%

Section: Conservation and Divergence Of Apc/c Activity In Meiosis Amomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Arabidopsis anaphase‐promoting complex/cyclosome subunit 8 is required for male meiosis

Wang

et al. 2019

New Phytologist

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“…SDS and TAM build active kinase complexes with CDKA;1, the Arabidopsis Cdk1/Cdk2 homolog, that is the main cell cycle regulator in Arabidopsis (Cromer et al, 2012;Nowack et al, 2012;Cifuentes et al, 2016). A function of CDKA;1 in meiosis is supported by the analysis of weak loss-of-function mutants, which are completely sterile (Dissmeyer et al, , 2009).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis Cdk1/Cdk2 homolog CDKA ;1 controls chromosome axis assembly during plant meiosis

et al. 2019

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Meiosis is key to sexual reproduction and genetic diversity. Here, we show that the Arabidopsis cyclin‐dependent kinase Cdk1/Cdk2 homolog CDKA;1 is an important regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis homolog of Hop1 (homolog pairing 1), essential for synaptonemal complex formation, as a target of CDKA;1. The phosphorylation of ASY1 is required for its recruitment to the chromosome axis via ASYNAPTIC 3 (ASY3), the Arabidopsis reductional division 1 (Red1) homolog, counteracting the disassembly activity of the AAA+ ATPase PACHYTENE CHECKPOINT 2 (PCH2). Furthermore, we have identified the closure motif in ASY1, typical for HORMA domain proteins, and provide evidence that the phosphorylation of ASY1 regulates the putative self‐polymerization of ASY1 along the chromosome axis. Hence, the phosphorylation of ASY1 by CDKA;1 appears to be a two‐pronged mechanism to initiate chromosome axis formation in meiosis.

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“…For AtREC8, there is currently no alternative as the single other gene known to be required for monopolar orientation of kinetochores is SCC3, which is also expressed mitotically and thus the corresponding mutation would affect regular development [19]. Two alternatives to osd1 can be used to create MiMe, tam and TDMΔD [17,21]. Finally, besides AtSPO11-1, several genes have been shown to be essential for initiation of meiotic recombination through DNA double-strand break formation (AtSPO11-2, PRD1, PRD2, PRD3/PAIR1, DFO and MTOPVIB) [22][23][24][25][26], but are not yet tested to create the MiMe phenotype.…”

Section: Introductionmentioning

confidence: 99%

Turning rice meiosis into mitosis

Mieulet¹,

Jolivet²,

Rivard³

et al. 2016

Cell Res

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132

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Introduction of clonal reproduction through seeds (apomixis) in crops has the potential to revolutionize agriculture by allowing self-propagation of any elite variety, in particular F1 hybrids. In the sexual model plant Arabidopsis thaliana synthetic clonal reproduction through seeds can be artificially implemented by (i) combining three mutations to turn meiosis into mitosis (MiMe) and (ii) crossing the obtained clonal gametes with a line expressing modified CENH3 and whose genome is eliminated in the zygote. Here we show that additional combinations of mutations can turn Arabidopsis meiosis into mitosis and that a combination of three mutations in rice (Oryza sativa) efficiently turns meiosis into mitosis, leading to the production of male and female clonal diploid gametes in this major crop. Successful implementation of the MiMe technology in the phylogenetically distant eudicot Arabidopsis and monocot rice opens doors for its application to any flowering plant and paves the way for introducing apomixis in crop species.

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TDM1 Regulation Determines the Number of Meiotic Divisions

Cited by 47 publications

References 45 publications

The Arabidopsis anaphase‐promoting complex/cyclosome subunit 8 is required for male meiosis

The Arabidopsis anaphase‐promoting complex/cyclosome subunit 8 is required for male meiosis

The Arabidopsis Cdk1/Cdk2 homolog CDKA ;1 controls chromosome axis assembly during plant meiosis

Turning rice meiosis into mitosis

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