TDZ induced micropropagation in Cymbidium giganteum Wall. Ex Lindl. and assessment of genetic variation in the regenerated plants

Roy, Aniruddha; Sajeev, Sudha; Pattanayak, A.; Deka, Bidyut C.

doi:10.1007/s10725-012-9732-0

Cited by 42 publications

(19 citation statements)

References 25 publications

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“…This study confirms the efficiency of TDZ and auxin combination. Similar effectiveness of TDZ and NAA concentration on shoot proliferation has been reported in Vanda coerulea and Cymbidium giganteum (Malabadi et al 2004;Roy et al 2012).…”

Section: Discussionsupporting

confidence: 81%

An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb.f.

Mahendran

2015

Plant Biosystems - An International Journal Dealing with all As

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An in vitro plant regeneration protocol was successfully established in Aphyllorchis montana, a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-1-Terrestrial Orchid medium was found to be the best followed by Knudson C medium. Half-strength BM-1-Terrestrial Orchid medium was supplemented with different growth regulators either individually or in combinations for multiplication of shoots induction. Of the five cytokinins tested, thidiazuron at 6.8 mM was found to be most effective for multiple shoot induction yielding 17.24^0.27 shoots after 10 weeks of culture. Addition of low concentration of a-Naphthaleneacetic acid (1.3 mM) in MS medium supplemented with the cytokinin thidiazuron (6.8 mM) favored shoot multiplication. The addition of organic additives to the medium containing thidiazuron enhanced the number of regenerated shoots. The plantlets were acclimatized, and the survival rate was 100%. Screening of the antioxidant and antimicrobial activities and estimation of total phenolics and flavonoid content of methanolic extracts of micropropagated plants were also carried out and compared with that of the wild-grown plants. In all the tests, methanolic extract from wild-grown plants showed higher antioxidant, antimicrobial activity, total phenolics and flavonoid content than in vitro propagated plants.

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Section: Discussionsupporting

confidence: 81%

An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb.f.

Mahendran

2015

Plant Biosystems - An International Journal Dealing with all As

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“…(Prakash et al, 2004), Cymbopogon flexuosus (Bhattacharya et al, 2008), papaya (Homhuan et al, 2008) and cotton (Jin et al, 2008). Also, there are several reports about TDZ affecting the DNA stability of plants propagated in vitro (Sarmast et al, 2012;Roy et al, 2012;Khattab et al, 2014). In contrast, the RAPD analysis performed in our study detected no polymorphism within plants regenerated through the same regeneration pathway, whereas the RAPD analysis with the same decamer primers revealed considerable polymorphism within iris plants derived from seeds.…”

Section: Discussioncontrasting

confidence: 68%

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

Stanišić

Raspor

Ninković

et al. 2015

South African Journal of Botany

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Efficient protocols, safe from somaclonal variation, were developed for regeneration of Iris sibirica plants via organogenesis and somatic embryogenesis from leaf-base explants cultivated on Murashige and Skoog media supplemented with thidiazuron (TDZ, 1.0 mg/l) or 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l). The morphogenic response and callus formation efficiency differed significantly between 2,4-D (80.9%) and TDZ (67%) morphogenesis induction treatments. TDZ induced only organogenic calli, while calli obtained with 2,4-D were composed of three types differing in color and consistency: white, friable -embryogenic calli (4.5%, 3.8 mg/explant), green, compact -organogenic calli (12.4%, 48.4 mg/explants) and yellow -nonregenerative calli (77.3%, 254.4 mg/explant). The cultivation of embryogenic calli on medium with 2,4-D and Kinetin resulted in further development of somatic embryos (54 embryos/g of calli) which germinated with a frequency of 62% after being transferred to a medium without plant growth regulators. Stable shoot cultures were established by transferring organogenic calli with shoot primordia to media with 0.1 mg/l α-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzylaminopurine (BA), while further cultivation on media of the same composition (TDZ or 2,4-D) resulted in the reduced growth and rhizogenesis, respectively. The TDZ induction treatment resulted in higher number of shoots per explant (7.9) than the 2,4-D treatment (4.3). After successful rooting and ex vitro acclimatization, plants were grown in the field and flowered to seed production. Flow cytometry, chromosome counting and random amplification of polymorphic DNA (RAPD) analysis indicated no evidence of genetic variation in plants regenerated via somatic embryogenesis or organogenesis. The results suggest that established protocols are safe for use in genetic transformation procedures or large-scale production of true-to-type I. sibirica plants.

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“…The ascendancy of TDZ in inducing somatic embryos (infant PLBs) from leaf explants was recounted in numerous orchid species including Oncidium (Mayer, Stancato, & Appezzato-Da-Glória, 2010), Doritaenopsis (Park, Yeung, Chakrabarty, & Paek, 2002), Dendrobium and Phalaenopsis (Kuo, Chen, & Chang, 2005). TDZ is also useful for rapid plant regeneration through organogenesis (Kishor & Devi, 2009;Jitsopakul, Thammasiri, & Ishikawa, 2013), somatic em-bryogenesis (Jheng, Do, Liauh, Chung, & Huang, 2006;Gow, Chen, & Chang, 2009), or PLB formation of orchids (Mayer, Stancato, & Appezzato-Da-Glória, 2010;Khoddamzadeh et al, 2011;Roy, Sajeev, Pattanayak, & Deka, 2012). NAA is a synthetic auxin plant growth regulator that is routinely used in in vitro propagation of orchid to promote callus induction such as in Dendrobium (Mei, Danial, Mahmood, & Subramaniam, 2012;Salam, Salam, & Mohanty, 2013), Vanilla (Tan, Chin, & Alderson, 2011), Oncidium (Mayer, Stancato, & AppezzatoDa-Glória, 2010), Phalaenopsis (Khoddamzadeh et al, 2011), and Renanthera (Wu et al, 2012).…”

Section: Effect Of Pgrs On Embryogenic Callus Induction From Leaf Tipmentioning

confidence: 99%

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

Jainol

Gansau

2017

Agrivita.J.Agr.Sci

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Dimorphorchis lowii is a rare epiphytic orchid endemic to Borneo and very popular due to its ornamental value. For the purpose of mass propagation, procedures for in vitro propagation through callus were developed. The objectives of this study were to determine the effects of plant growth regulators (PGRs) on callus induction from leaf tip explant and the effects of complex additives on PLBs formation and shoot development. The best combination of PGR was 3.0 mg L -1 Thidiazuron (TDZ) with 0.046 mg L -1 NAA supplemented in half-strength medium (½ MS) (Murashige & Skoog, 1962). The percentage of survival and callus formation obtained were 96.0 % ± 19.8 and 52.0 % ± 16.5 respectively. Maximum shoot proliferation from PLBs was observed in Knudson C (KC) medium enriched with 15 % (v/v) coconut water. In this treatment, 10.2 ± 6.2 shoots were produced from one callus explant. Shoots were rooted on KC medium containing 15 % (v/v) coconut water and transferred in a medium mixture containing sphagnum moss, charcoal, brick and coco peat with the ratio of 1:1:1:1. After 2 months being acclimatized in the glasshouse, 78 % of plantlets survived. Histology observations showed that embryogenic callus derived from leaf tip explants might originate from epidermis and mesophyll cells and was capable of developing into complete plantlets.

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TDZ induced micropropagation in Cymbidium giganteum Wall. Ex Lindl. and assessment of genetic variation in the regenerated plants

Cited by 42 publications

References 25 publications

An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb.f.

An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb.f.

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

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