TDZ-induced plant regeneration in Brassica oleracea L. var. botrytis: effect of antioxidative enzyme activity and genetic stability in regenerated plantlets

Srivastava, Shalini; Krishna, Ram; Sinha, Rajeshwar P.; Singh, Mahesh Kumar

doi:10.1007/s11627-017-9861-2

Cited by 14 publications

(3 citation statements)

References 37 publications

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“…Also, the fidelity among regenerated plants using ISSR-PCR-based was reported in Eleusine coracana [46] and Morus spp [47]. Consistency, several studies assessed the genetic fidelity in in vitro regenerants was reported in other plant species as well, such as Prunus dulcis [48], Phoenix dactylifera [49], Jatropha curcas [50], Brassica oleracea [51], and Rhodiola imbricate [52].…”

Section: Discussionmentioning

confidence: 92%

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

Shalan¹,

Soliman²,

Mahmoud³

et al. 2023

Phyton

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Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication. In this research, nine multiplication media (MM) augmented with different concentrations of 6-benzyl adenine (BA), Kinetin (Kin), and Thidiazuron (TDZ), Three rooting media (RM) supplemented with three levels of α-naphthalene acetic acid (NAA) and three types of soil mixtures (v/v); Coco peat/Vermiculite/Sand (CVS), Peat moss/Perlite/Sand (PPS) and Peat moss/Perlite (PP) were used in the micropropagation protocol of daylily plants. MM2 showed the maximum shoot length and the number of leaves, while MM9 showed the maximum number of shoots. The RM1 showed the maximum root length and the number of roots. During acclimatization, CVS, PPS, and PP soil mixture showed similar performance except the CVS mixture showed lower performance regarding plant height and diameter. The genetic fidelity of micropropagated plants was evaluated using Start Codon Targeted (SCoT) Markers. Six SCoT primers amplified 51 scorable bands with an approximate range from 146 bp to 1598 bp size. Thirty one out of 51 loci were presented in the mother plants. 40 loci were polymorphic, 11 were monomorphic and 7 were unique. The amplification patterns of the micropropagated plants demonstrated genetic integrity to the mother plant ranging from 84.32 to 47.06 and somaclonal variations ranging from 52.94 with 5 mg/l BA pathway to 15.68 with 1mg/l TDZ pathway, thus demonstrating that the homogeneity and the variation of the micropropagated plants affected by the type and the quantity of the plant growth regulator used during multiplication subcultures. This research can be successfully used for other ornamental and medicinal plants' bulk multiplication, germplasm conservation, and future genetic improvement.

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Section: Discussionmentioning

confidence: 92%

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

Shalan¹,

Soliman²,

Mahmoud³

et al. 2023

Phyton

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“…A careful selection of the TDZ concentration is essential because it can stimulate endogenous ethylene production [34,35]. Singh et al [36] achieved optimal callus growth with 1.2 mg•L −1 TDZ alone in Santalum album L. Srivastava et al [37] induced up to 85.56% callus using 9.1 µM of TDZ and 0.5 µM of NAA. In our study, we successfully obtained calli with 2.5 mg•L −1 TDZ, which were non-browning and had excellent growth.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Production of Secondary Metabolites from a Halophyte Tetragonia tetragonoides through Callus Culture

Lee,

Nam,

Jeon

et al. 2024

Horticulturae

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Considering Korea’s gradual shift toward an aging society, consumer interest in compounds with physiological benefits, including antioxidant and anticancer effects, has surged. This study explored the potential of Tetragonia tetragonoides (Pall.) Kunze, commonly known as New Zealand Spinach (NZS), a halophyte with reported health benefits, including efficacy in treating gastrointestinal diseases, high blood pressure, diabetes, and obesity. This study also introduced a novel callus culture system for NZS, allowing for the rapid in vitro production of secondary metabolites. Optimal callus induction (100%) and biomass production (0.416 g) were achieved by adding 2.0 mg·L−1 6-BA (6-Benzylaminopurine) and 0.5 mg·L−1 2,4-D (2,4-Dichlorophenoxyacetic acid) among five auxin and cytokinin combinations. Two distinct callus types, TGC [TDZ (Thidiazuron)-supplemented Green Callus] and TNYC [TDZ + NAA (Naphthalene acetic acid)-supplemented Yellow Callus], were identified, each with unique characteristics. The calli showed total phenolic and flavonoid contents comparable to those of NZS leaves grown in the greenhouse. An expression analysis of six genes (CHS, CHI, F3H, F3′H, FLS, and DFR) involved in the kaempferol biosynthesis revealed an enhanced flavonoid biosynthesis-related gene expression in TGC, emphasizing its potential for compound production. GC-MS analysis identified distinct compound profiles in TGC and TNYC, with 2,3-butanediol and succinic acid being the predominant compounds among the nine and forty-four components, respectively. These calli offer a stable supply of functional compounds and present an environmentally sustainable solution. The derived callus culture system is anticipated to contribute to the development of healthy functional foods or pharmaceuticals from halophyte NZS.

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“…Also, Wójcik et al [76] employed two DNA-based approaches to assess genetic stability of micropropagated plants, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), and found no polymorphism among the analyzed gooseberry cultivars. Other plant species with consistent genetic fidelity in in vitro regenerates include Prunus ulcis [77], Brassica oleracea [78], and Rhodiola imbricate [79].…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

Nower

2022

Journal of Genetic Engineering and Biotechnology

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Background Dimocarpus longan is a tropical tree that produces edible fruit. It is a neglected plant species that is listed as near threatened. In spite of its economic value, the propagation of longan cultivar using conventional methods is extremely difficult. The goal of this research is to produce and conserve this plant through in vitro propagation. Results In order to form new shoots, sterilized shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (BA) or 2-isobentenyl-adenine (2ip). For direct organogenesis, young leaves of new shoots were cultured on MS medium fortified with various concentrations of Thidiazuron (TDZ) or 6-(4-Hydroxy-3-methylbut-2-enylamino purine) (Zeatin). Gibbrellic acid (GA3) at different levels alone or in combination was used for shoot elongation. Also, indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) were used for root formation. MS medium supplemented with 1.00 mg/l 2ip was suitable for inducing axillary shoots from shoot tips (4.0 axillary shoots/explant). The highest significant 76% and numbers of adventitious buds from leaf base were achieved on MS medium containing 1.0 mg/l TDZ. These buds developed into the longest plantlets on GA3 at 3.0 mg/l and rooted well in ½MS containing 1.50 mg/l IBA plus 0.50 mg/l (NAA). About 70% in vitro plants were successfully acclimatized. The AFLP profile illustrated the genetic stability of gene expression action. The amplified fragment length polymorphisms (AFLPs) profile illustrated the progenies were extremely similar to the mother plants. According to our findings, MS medium containing 25 ppm salicylic acid (SA) and 5 ppm methyl jasmonate (MeJA) produced the highest percentage of apigenin in longan calli (77.09 and 2.637%, w/w). Conclusion A successful and efficient micropropagation protocol has been developed and described here for the first time, and it will be very useful for the clonal propagation and conservation of the near-threatened Dimocarpus longan plant. Micropropagated plants are genetically identical to the donor plant using the AFLP technique. The usefulness of salicylic acid and methyl jasmonate as elicitors for increasing in vitro production of secondary metabolites in plants is demonstrated in this work.

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TDZ-induced plant regeneration in Brassica oleracea L. var. botrytis: effect of antioxidative enzyme activity and genetic stability in regenerated plantlets

Cited by 14 publications

References 37 publications

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

Exploring the Production of Secondary Metabolites from a Halophyte Tetragonia tetragonoides through Callus Culture

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

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