Tea Culture in Deposit in Vitro-Collection of the Russian Research Institute of Floriculture and Subtropical Crops

Gvasaliya, M. V.

doi:10.31360/2225-3068-2019-68-119-125

Cited by 2 publications

(2 citation statements)

References 3 publications

(3 reference statements)

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“…For tea plant cv. Kolkhida, micropropagation protocols were developed; however, SE and AT protocols have not been established [ 29 , 30 ]. The aim of this study was to search for the optimal Agrobacterium tumefaciens -mediated transfection protocol for the Kolkhida tea variety and semi-quantify its efficiency.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium Transformation of Tea Plants (Camellia sinensis (L.) KUNTZE): A Small Experiment with Great Prospects

Fizikova,

Subcheva,

Kozlov

et al. 2024

Plants

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Tea has historically been one of the most popular beverages, and it is currently an economically significant crop cultivated in over 50 countries. The Northwestern Caucasus is one of the northernmost regions for industrial tea cultivation worldwide. The domestication of the tea plant in this region took approximately 150 years, during which plantations spreading from the Ozurgeti region in northern Georgia to the southern city of Maykop in Russia. Consequently, tea plantations in the Northern Caucasus can serve as a source of unique genotypes with exceptional cold tolerance. Tea plants are known to be recalcitrant to Agrobacterium-mediated transfection. Research into optimal transfection and regeneration methodologies, as well as the identification of tea varieties with enhanced transformation efficiency, is an advanced strategy for improving tea plant culture. The aim of this study was to search for the optimal Agrobacterium tumefaciens-mediated transfection protocol for the Kolkhida tea variety. As a result of optimizing the transfection medium with potassium phosphate buffer at the stages of pre-inoculation, inoculation and co-cultivation, the restoration of normal morphology and improvement in the attachment of Agrobacterium cells to the surface of tea explants were observed by scanning electron microscopy. And an effective method of high-efficiency Agrobacteria tumefaciens-mediated transfection of the best local tea cultivar, Kolkhida, was demonstrated for the first time.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium Transformation of Tea Plants (Camellia sinensis (L.) KUNTZE): A Small Experiment with Great Prospects

Fizikova,

Subcheva,

Kozlov

et al. 2024

Plants

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“…Генетические коллекции ВНИИЦиСК на конец 2019 г. насчитывали 2 600 сортообразцов цветочно-декоративных, субтропических и южных плодовых культур (рис. 1), которые используются для изучения адаптивности культур по отношению к стрессорам абиотической и биотической природы, оценки их биологического потенциала, разработки схем рационального размещения, технологий сортовой агротехники, получения сортов отечественной селекции [5,7,10,17,27,35,38,41]. В прошедшем году генетические коллекции Института пополнились 54 сортообразцами красивоцветущих кустарников, 10 сортами пеларгоний и 2 сортообразцами травянистых растений для зимнего сада.…”

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Research Findings of the Russian Research Institute of Floriculture and Subtropical Crops in 2019

Ryndin¹,

Карпун²,

Слепченко³

2020

SOH

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The paper discusses some problems of postgraduate education development at the present stage. The authors argue for the necessity to introduce psychological and pedagogical knowledge into postgraduate education programs as well as into professional development programs at a more extensive level. It is offered to practice certain forms of psychological and pedagogical work within the framework of professional development programs for teachers. Recommendations are also given on students' psychological characteristics that should be taken into account. The authors offer to include the creativity development training across the curriculum, which will involve the knowledge of creative psychology; furthermore, it is offered to develop a similar program for teaching staff, since appropriate psychological and pedagogical skills will optimize the joint work of a teacher and a postgraduate student on solving various research problems.

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Tea Culture in Deposit in Vitro-Collection of the Russian Research Institute of Floriculture and Subtropical Crops

Cited by 2 publications

References 3 publications

Agrobacterium Transformation of Tea Plants (Camellia sinensis (L.) KUNTZE): A Small Experiment with Great Prospects

Agrobacterium Transformation of Tea Plants (Camellia sinensis (L.) KUNTZE): A Small Experiment with Great Prospects

Research Findings of the Russian Research Institute of Floriculture and Subtropical Crops in 2019

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