Tea polyphenols inactivate Cronobacter sakazakii isolated from powdered infant formula

Li, R.; Fei, P.; Man, Chaoxin; Lou, Binbin; Niu, J.T.; Feng, Jing; Sun, Luoting; Li, M.Y.; Jiang, Yujun

doi:10.3168/jds.2015-10039

Cited by 43 publications

(20 citation statements)

References 40 publications

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“…In addition, tea polyphenols at 5 mg/mL could eliminate approximately 7.0 log CFU/mL of C . sakazakii within 1 h [ 28 ]. Comparing with most of these plant-derived compounds, citral exhibited a stronger antimicrobial activity against C .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii

et al. 2016

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Citral is a flavor component that is commonly used in food, beverage and fragrance industries. Cronobacter sakazakii is a food-borne pathogen associated with severe illness and high mortality in neonates and infants. The objective of the present study was to evaluate antimicrobial effect of citral against C. sakazakii strains. The minimum inhibitory concentration (MIC) of citral against C. sakazakii was determined via agar dilution method, then Gompertz models were used to quantitate the effect of citral on microbial growth kinetics. Changes in intracellular pH (pHin), membrane potential, intracellular ATP concentration, and membrane integrity were measured to elucidate the possible antimicrobial mechanism. Cell morphology changes were also examined using a field emission scanning electron microscope. The MICs of citral against C. sakazakii strains ranged from 0.27 to 0.54 mg/mL, and citral resulted in a longer lag phase and lower growth rate of C. sakazakii compared to the control. Citral affected the cell membrane of C. sakazakii, as evidenced by decreased intracellular ATP concentration, reduced pHin, and cell membrane hyperpolarization. Scanning electron microscopy analysis further confirmed that C. sakazakii cell membranes were damaged by citral. These findings suggest that citral exhibits antimicrobial effect against C. sakazakii strains and could be potentially used to control C. sakazakii in foods. However, how it works in food systems where many other components may interfere with its efficacy should be tested in future research before its real application.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii

et al. 2016

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“…Cronobacter sakazakii ( C. sakazakii ) is an emerging opportunistic foodborne pathogen that can infect infants and neonates resulting in meningitis, necrotizing enterocolitis, and bacteremia, with a 40–80% mortality rate [ 1 , 2 ]. Methods to detect foodborne pathogens as potential biological threats to our health and economy have increasingly become a priority [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii

Aly

Domig

Kneifel

et al. 2018

Sensors

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Cronobacter sakazakii is a foodborne pathogen that can cause a rare, septicemia, life-threatening meningitis, and necrotizing enterocolitis in infants. In general, standard methods for pathogen detection rely on culture, plating, colony counting and polymerase chain reaction DNA-sequencing for identification, which are time, equipment and skill demanding. Recently, nanoparticle- and surface-based immunoassays have increasingly been explored for pathogen detection. We investigate the functionalization of gold nanoparticles optimized for irreversible and specific binding to C. sakazakii and their use for spectroscopic detection of the pathogen. We demonstrate how 40-nm gold nanoparticles grafted with a poly(ethylene glycol) brush and functionalized with polyclonal antibodies raised against C. sakazakii can be used to specifically target C. sakazakii. The strong extinction peak of the Au nanoparticle plasmon polariton resonance in the optical range is used as a label for detection of the pathogens. Individual binding of the nanoparticles to the C. sakazakii surface is also verified by transmission electron microscopy. We show that a high degree of surface functionalization with anti-C. sakazakii optimizes the detection and leads to a detection limit as low as 10 CFU/mL within 2 h using a simple cuvette-based UV-Vis spectrometric readout that has great potential for further optimization.

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“…Cronobacter spp. are emerging foodborne opportunistic pathogens that can infect neonates and infants resulting in necrotizing enterocolitis, bacteremia, and meningitis, with a 40–80% mortality rate (Holy and Forsythe, 2014 ; Li et al, 2016 ). These organisms have be isolated from various food sources, including spiced meat, ready-to-eat foods, dehydrated rice powder, retail foods, and powdered infant formula (PIF) (Iversen and Forsythe, 2004 ; Hochel et al, 2012 ; Joseph et al, 2012a ; Huang et al, 2015 ; Xu et al, 2015 ; Zhang et al, 2016 ; Brandão et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Cronobacter sakazakii Isolates from Powdered Infant Formula Collected from Chinese Retail Markets

Peng

Jiang

Jiang³

et al. 2017

Front. Microbiol.

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Cronobacter sakazakii is an opportunistic pathogen that causes severe infections in neonates and infants through contaminated powdered infant formula (PIF). Therefore, the aim of this study was a large-scale study on determine the prevalence, molecular characterization and antibiotic susceptibility of C. sakazakii isolates from PIF purchased from Chinese retail markets. Two thousand and twenty PIF samples were collected from different institutions. Fifty-six C. sakazakii strains were isolated, and identified using fusA sequencing analysis, giving a contamination rate of 2.8%. Multilocus sequence typing (MLST) was more discriminatory than other genotyping methods. The C. sakazakii isolates were divided into 14 sequence types (STs) by MLST, compared with only seven clusters by ompA and rpoB sequence analysis, and four C. sakazakii serotypes by PCR-based O-antigen serotyping. C. sakazakii ST4 (19/56, 33.9%), ST1 (12/56, 21.4%), and ST64 (11/56, 16.1%) were the dominant sequence types isolated. C. sakazakii serotype O2 (34/56, 60.7%) was the primary serotype, along with ompA6 and rpoB1 as the main allele profiles, respectively. Antibiotic susceptibility testing indicated that all C. sakazakii isolates were susceptible to ampicillin-sulbactam, cefotaxime, ciprofloxacin, meropenem, tetracycline, piperacillin-tazobactam, and trimethoprim-sulfamethoxazole. The majority of C. sakazakii strains were susceptible to chloramphenicol and gentamicin (87.5 and 92.9%, respectively). In contrast, 55.4% C. sakazakii strains were resistant to cephalothin. In conclusion, this large-scale study revealed the prevalence and characteristics of C. sakazakii from PIF in Chinese retail markets, demonstrating a potential risk for neonates and infants, and provide a guided to effective control the contamination of C. sakazakii in production process.

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Tea polyphenols inactivate Cronobacter sakazakii isolated from powdered infant formula

Cited by 43 publications

References 40 publications

Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii

Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii

Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii

Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Cronobacter sakazakii Isolates from Powdered Infant Formula Collected from Chinese Retail Markets

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