The phytosterols are naturally occurring, physiologically active substances found in foods of plant origin. In present study, the phytosterols profile of four bi-clonal tea seed stock oils viz., TS 462, TS 464, TS 491, and TS 506 released from Tocklai are presented. Phytosterols were evaluated using GC-FID. Among the phytosterols, 24-methylene cycloartan-3-ol was recorded as the most abundant sterol in tea seed oil, accounting for 31% of total phytosterols. Results showed that the majority of the sterol differences were significant (p 0.05). TS 506 has the highest 24- methylene cycloartan-3-ol (476.03.7 mg kg-1) followed by TS 491 (475.97 mg kg-1). β-sitosterol, cycloartenol, canophyllol, lanosterol and campesterol were also present with their contents of 371.03 ± 0.26, 291.38 ± 0.12, 120.44 ± 0.13, 89.12 ± 0.19 and 69.05 ± 0.24 mg kg-1 respectively. The outcomes of the present study provided a foundational understanding of the phytosterols profiles of the four bi-clonal tea seed oils and highlighted their similarities and differences.