IntroductionThe COVID-19 pandemic has had repercussions on teachers’ beliefs about their efficacy in their profession. In light of this, the aim of this study was to determine the effect of perceived stress, job satisfaction, and workload on professional self-efficacy among Peruvian regular basic education teachers.MethodThis was a cross-sectional explanatory study involving 687 regular basic education teachers (57.6% women) aged between 23 and 55 years (M = 38.15, SD = 8.58), from both private and public institutions across the three regions of Peru (coastal, jungle and highland). The instruments used were the Professional Self-Efficacy Questionnaire (AU-10), the Perceived Stress Scale related to the pandemic (EEP-10), the Affective Job Satisfaction Scale (BIAJS), and the Workload Scale (ECT).ResultsA SEM analysis was conducted, yielding satisfactory goodness-of-fit indices: χ2 = 87.028, p = 0.000, with 11 df, CFI = 0.993, RMSEA = 0.047, and SRMR = 0.021. This confirmed H1, as there is a negative effect of workload on professional self-efficacy (β = −0.11, p = 0.017). Similarly, H2 was confirmed, as there is a positive effect of job satisfaction on professional self-efficacy (β = 0.13, p = 0.003). However, H3 was rejected, as no significant effect of perceived stress on professional self-efficacy was evident (β = −0.02, p = 0.658).ConclusionWorkload and job satisfaction explain the perceived level of self-efficacy among Peruvian regular basic education teachers. However, there is no evidence to suggest that stress affects their sense of efficacy.