Although there have been studies of individual professional disciplines and their attitudes toward children with emotional, behavioral, or educational problems, there has been no systematic determination using a single, psychometrically sound measure that compared different professional groups. The goal was to assess the biases toward children with emotional and behavioral difficulties in samples of professionals who are most likely to have contact with them (86 teachers, 83 psychologists, 47 social workers), as well as other adults (75 undergraduate students). After development from an item pool, the provisional Biases toward Children with Psychological and Behavioral Disorders scale consisted of 21 items in one factor which experts also had assigned to a Biases category. The scale had good internal consistency reliability (alpha = .84). Validity was supported by a team of experts, factor structure, and known-groups comparisons. Psychologists and social workers scored lower than did teachers and students.