Tear-based MMP-9 detection: A rapid antigen test for ocular inflammatory disorders using vanadium disulfide nanowires assisted chemi-resistive biosensor

Ghosh, Tanmoya Nemai; Rotake, Dinesh; Kumar, Saurabh; Kaur, Inderjeet; Singh, Shiv Govind

doi:10.1016/j.aca.2023.341281

Cited by 7 publications

(3 citation statements)

References 57 publications

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“…To achieve a repeatable biosensor response, notably, the composite-DMF dispersion was dropped cast in 1.5 μL volume at three steps (0.5 μL × 3) on top of the IDEs to mitigate the device variability . Additionally, during the drop-casting steps, the biosensor’s substrate was heated at 60 °C, reported effective in variability reduction . Finally, the response of the biosensor device is evaluated from the calibration curve prepared from the experiments with known concentrations of the control samples.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…36 Additionally, during the drop-casting steps, the biosensor's substrate was heated at 60 °C, reported effective in variability reduction. 37 Finally, the response of the biosensor device is evaluated from the calibration curve prepared from the experiments with known concentrations of the control samples. For preparing the calibration curve, the current values obtained for the selected voltage range with the voltage selection algorithm had been used.…”

Section: Synthesis Of Cnt-zno Compositementioning

confidence: 99%

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Device-Physics Realization of ZnO–MWCNT Nanostructure-Based Field-Effect Biosensor for Ultrasensitive Simultaneous Genomic Detection of Foodborne Pathogens

Goswami,

Bonam,

Jeyaram

et al. 2023

Anal. Chem.

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The complex and versatile interactions among the wide variety of the nanostructures and the target analytes have primarily limited the detailed investigation of the transduction mechanism of nanomaterial-assisted electrical signal-based biosensors despite their high sensitivity, low-cost, portability, and ease of deployment. Hence, no common ground is formed detailing the principle of operation, demanding a strong need for systematic examination instead of hit and trial. Therefore, a maiden mechanistic investigation has been carried out in this paper for a field-effect-based biosensor device relying on the energy band diagram and the surface potential profile. To demonstrate the experimental evidence and appreciate the importance of food safety, three hazardous foodborne pathogens (Proteus mirabilis, Escherichia coli, and Clostridium botulinum) have been detected herein. The biosensor device, built on a hydrothermally synthesized zinc oxide and MWCNT (ZnO−MWCNT) composite nanostructure, simultaneously incorporates three fairly specific ss-DNA probes. Furthermore, the unmet challenge of biosensor device variability is addressed through the optimum selection of operating voltage of the device via a unique "voltage−selection−algorithm". We believe that the rigorous experimentation and the insightful device-physics realization demonstrated in this work will pave the way for a future decisive biosensor platform.

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Section: Experimental Methodsmentioning

confidence: 99%

Section: Synthesis Of Cnt-zno Compositementioning

confidence: 99%

Device-Physics Realization of ZnO–MWCNT Nanostructure-Based Field-Effect Biosensor for Ultrasensitive Simultaneous Genomic Detection of Foodborne Pathogens

Goswami,

Bonam,

Jeyaram

et al. 2023

Anal. Chem.

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“…These powders resulted in different morphology (section 3.1: characterization of VS 2 ), as 2D nanostructure showed superior electrochemical performance-it was chosen for the construction of biosensors. This methodology of 2D VS 2 nanosheet synthesis with some modifications has been reported in previous works [31][32][33].…”

Section: Synthesis Of 2d Vs 2 Nanosheetsmentioning

confidence: 99%

2D vanadium disulfide nanosheets assisted ultrasensitive, rapid, and label-free electrochemical quantification of cancer biomarker (MMP-2)

2023

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Cancer is one of the most tormenting global health burdens reporting high mortality and morbidity worldwide. Matrix metalloproteinase 2 (MMP-2) protein has elevated expression for most types of cancers, including prostate and breast cancer. Therefore, accurate and specific detection of MMP-2 biomarker is crucial for screening, treatment, and prognosis of related cancer. In this work, we have proposed a label-free electrochemical biosensor for the detection of MMP-2 protein. This biosensor was fabricated using hydrothermally synthesized vanadium disulfide (VS2) nanosheets with monoclonal anti-MMP2 antibodies biofunctionalized using a suitable linker. The VS2 nanomaterials were synthesized hydrothermally at different reaction temperatures (140, 160, 180 and 200) generating different morphologies from a 3-D bulk cubic structure at 140C to 2-D nanosheets at 200C. Owing to the advantages of 2-D VS2 nanosheets with high surface-to-volume ratio, excellent electrochemical response and high antibody loading possibility, it was selected for fabricating an MMP-2 specific biosensor. The antibody-antigen binding event is analyzed by recording electrochemical impedance spectroscopy (EIS) signals for different target MMP-2 protein concentrations. The sensitivity and lower limit of detection (LOD) were 7.272 (R/R)(ng/mL)-1cm-2 and 0.138 fg/mL, respectively in 10 mM phosphate buffer saline (PBS) for this proposed sensor. Further, interference studies were also performed which demonstrates the sensor to be highly selective against non-specific target proteins. This 2-D VS2 nanosheet-based electrochemical biosensor is a sensitive, cost-effective, accurate, and selective solution for cancer diagnosis.

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A Flexible and Wearable Chemiresistive Biosensor Fabricated by Laser Inducing for Real‐Time Glucose Analysis of Sweat

Sun

Song

Zhao

et al. 2023

Adv Materials Inter

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In this study, a flexible and wearable chemiresistive biosensor (FWCB) is developed for the real‐time analysis of glucose in sweat on the human skin surface based on a novel detection strategy of p‐type reduced graphene oxide (rGO) sensing film, which met the requirements of rapid, nondestructive testing. The proposed FWCB is fabricated in the form of interdigital electrodes (IEs) made of laser‐induced graphene (LIG) synthesized by the laser inducing of a polyimide (PI) film. Additionally, a semiconducting rGO sensing film modified on the surface of IEs is synthesized by thermal reduction of graphene oxide (GO), which is functionalized with glucose oxidase (GOx) by chemical cross‐linking to obtain GOx/FWCB. Moreover, the key parameters for FWCB fabrication are optimized, and the sensing strategy of the proposed GOx/FWCB is also investigated. The results show that the proposed GOx/FWCB can be used for the detection of glucose in the range of 0.01–3.0 mM with satisfactory selectivity, and the limit of detection (LOD) is calculated to be as low as 0.8 µM (S/N = 3). These dramatic advantages endow the proposed FWCB with broad application prospects in the field of portable, wearable, and real‐time detection of glucose in human sweat for health monitoring.

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Tear-based MMP-9 detection: A rapid antigen test for ocular inflammatory disorders using vanadium disulfide nanowires assisted chemi-resistive biosensor

Cited by 7 publications

References 57 publications

Device-Physics Realization of ZnO–MWCNT Nanostructure-Based Field-Effect Biosensor for Ultrasensitive Simultaneous Genomic Detection of Foodborne Pathogens

Device-Physics Realization of ZnO–MWCNT Nanostructure-Based Field-Effect Biosensor for Ultrasensitive Simultaneous Genomic Detection of Foodborne Pathogens

2D vanadium disulfide nanosheets assisted ultrasensitive, rapid, and label-free electrochemical quantification of cancer biomarker (MMP-2)

A Flexible and Wearable Chemiresistive Biosensor Fabricated by Laser Inducing for Real‐Time Glucose Analysis of Sweat

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