Tear Lipocalin and Lysozyme in Sjögren and Non-Sjogren Dry Eye

Caffery, Barbara; Joyce, EM; Boone, Adrienne N.; Slomovic, Allan R.; Simpson, Trefford; Jones, Lyndon; Senchyna, Michelle

doi:10.1097/opx.0b013e318181ae4f

Cited by 56 publications

(43 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lipocalin decrease is in agreement with previous studies performed in patients suffering from Sjogren's Syndrome 28 and Meibomian Gland Dysfunction. 29 To our knowledge, the decrease in the abundances of lipophillin A-B observed in our study has never been described before and may constitute a further progress in the identification of mechanisms responsible for the stability of human tears.…”

supporting

confidence: 82%

Tear proteomics in evaporative dry eye disease

Versura

Nanni

Bavelloni

et al. 2010

Eye

142

114

View full text Add to dashboard Cite

Purpose: To analyze tear protein variations in patients suffering from dry eye symptoms in the presence of tear film instability but without epithelial defects. Methods: Five microlitres of non-stimulated tears from 60 patients, suffering from evaporative dry eye (EDE) with a break-up time (BUT) o10 s, and from 30 healthy subjects as control (no symptoms, BUT 410 s) were collected. Tear proteins were separated by mono and bi-dimensional SDS-PAGE electrophoresis and characterized by immunoblotting and enzymatic digestion. Digested peptides were analyzed by liquid chromatography coupled to electrospray ionization quadrupole-time of flight mass spectrometry followed by comparative data analysis into Swiss-Prot human protein database using Mascot. Statistical analysis were performed by applying a t-test for independent data and a MannWhitney test for unpaired data (Po0.05). Results: In EDE patients vs controls, a significant decrease in levels of lactoferrin (data in % ± SD): 20.15 ± 2.64 vs 24.56 ± 3.46 (P ¼ 0.001), lipocalin-1: 14.98±2.70 vs 17.73±2.96 (P ¼ 0.0001), and lipophilin A-C: 2.89±1.06 vs 3.63±1.37 (P ¼ 0.006) was revealed, while a significant increase was observed for serum albumin: 9.45 ± 1.87 vs 3.46 ± 1.87 (P ¼ 0.0001). No changes for lysozyme and zinc a-2 glycoprotein (P ¼ 0.07 and 0.7, respectively) were shown. Proteomic analysis showed a downregulation of lipophilin A and C and lipocalin-1 in patients, which is suggested to be associated with post-translational modifications. Conclusions: Data show that tear protein changes anticipate the onset of more extensive clinical signs in early stage dry eye disease.

show abstract

supporting

confidence: 82%

Tear proteomics in evaporative dry eye disease

Versura

Nanni

Bavelloni

et al. 2010

Eye

142

114

View full text Add to dashboard Cite

show abstract

“…The total protein was significantly reduced compared to the controls. Previous studies have demonstrated the reduction of major tear proteins such as lysozyme, lactoferrin, lipocalins in DES condition [27]. In this study, differential expression of the proteins in both DES conditions was observed by 2-D approach in tear collected by Schirmer method without pooling of the specimen.…”

Section: Discussionmentioning

confidence: 84%

Two dimensional electrophoretic analysis of human tears: Collection method in dry eye syndrome

Saijyothi

Angayarkanni

Syama³

et al. 2010

Electrophoresis

View full text Add to dashboard Cite

Tear proteomics, by 2-DE, can give a fingerprint of the protein profile, which is well suited in clinical proteomics for biomarker identification and in diagnostics. The mode of tear collection can influence the representation of the proteins in the tear and therefore it is important to use the appropriate method. In this study, capillary and Schirmer mode of tear collection was done in the healthy controls and the Schirmer method was validated in dry eye syndrome conditions. 2-D PAGE of normal and dry eye tear was performed using pH 3-10 linear IPG strips followed by 13% SDS-PAGE. The spot intensity was analyzed by the PD quest software. The two methods were compared using Bland-Altman statistical tool. The 2-D map of capillary and Schirmer tear showed 147 ± 8 spots and 145 ± 7 spots respectively. Both the collection methods were in agreement with each other and were comparable. Dry eye tear protein showed differential expression of proteins as observed in 25-35 kDa region. One of the significantly reduced protein was identified as proline-rich 4 protein. Schirmer method of tear collection is reliable in patients with dry eye, which can display the differential protein expression and help in biomarker identification.

show abstract

“…[10] In patients with dry eye syndrome, tear components change in that lactoferrin lysozyme and tear lipocalin are decreased. [30][31][32][33] Therefore, it is reasonable that the bacterial colonization rate in ocular surface increases in patients with dry eye when compared with the normal population. Also, the activity of matrix metalloproteinase-9 (MMP-9) is increased in ocular surface of patients with dry eye.…”

Section: Discussionmentioning

confidence: 99%

Study of Conjunctival Flora in Patients after Peripheral Blood Stem Cell Transplantation and its Correlation with Tear Secretion

Chen¹,

Kuo²,

Wang³

et al. 2012

Biomedical

View full text Add to dashboard Cite

Scientific background of the subjectDry eye occurs in 40-76% of post-peripheral blood stem cell transplantation (PBSCT) patients. The rate of positive conjunctival swab cultures in dry eye patients increases when compared with normal control, and the mean bacterial count is positively correlated with the reduction in goblet cells. What this study adds to the fieldThere is a positive correlation between the colonization rate and the severity of dry eye in post-PBSCT patients.Coagulase-negative Staphylococci is the most common colonizing bacteria of the ocular surface in both the normal population and post-PBSCT patients. The spectrum of conjunctival flora is independent of patient immune status.Background: Dry eye is a major ocular complication of peripheral blood stem cell transplantation (PBSCT) and may predispose bacterial colonization to the conjunctiva. To investigate the conjunctival bacterial flora in patients receiving PBSCT, we encompassed patients who received PBSCT at least 1 year from 2002 to 2008 in this cross-sectional study. Methods:Patients were divided into three groups in accordance to the result of the Schirmer Ia test. In the control group, we enrolled dry-eye patients with underlying disease other than hematopoietic stem cell transplantation of which the age range was similar to the study group. Results:Thirty-six patients with 72 eyes were included in our study. The culture rates were 22% (8 in 36) in the first group (Schirmer Ia= 0-5 mm), 20% (4 in 20) in the second group (Schirmer Ia= 6-9 mm), and 0% (n=16) in the third group (Schirmer Ia ≧ 10 mm). The flora in patients receiving PBSCT were coagulase-negative Staphylococci, Staphylococcus aureus and Corynebacterium sp. The bacterial colonization rate in the post-PBSCT group was not higher than the control group (22.2% vs. 30.8%), and coagulase-negative Staphylococci was the most common flora in the control group. Conclusions: In conclusion, despite not having statistical significance, there seems to be a positive correlation between the colonization rate and the severity of dry eye. However, bacterial profile isolated in post-PBSCT patients is not significantly different from other dry eye patients. (Biomed J 2012;35:493-99)

show abstract

Tear Lipocalin and Lysozyme in Sjögren and Non-Sjogren Dry Eye

Cited by 56 publications

References 39 publications

Tear proteomics in evaporative dry eye disease

Tear proteomics in evaporative dry eye disease

Two dimensional electrophoretic analysis of human tears: Collection method in dry eye syndrome

Study of Conjunctival Flora in Patients after Peripheral Blood Stem Cell Transplantation and its Correlation with Tear Secretion

Contact Info

Product

Resources

About