Tears and aqueous humor from horses inoculated with contain antibodies which bind to cornea

Parma, A; Fernández, A.; Santisteban, Carlos; Bowden, Robert A.; Cerone, Silvia

doi:10.1016/0165-2427(87)90052-3

Cited by 46 publications

(27 citation statements)

References 4 publications

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“…Equine recurrent uveitis appears to be an autoimmune disease (358,443), and Faine (211) suggested that late-onset uveitis in humans may result from an autoimmune reaction to subsequent exposure. Immune involvement in retinal pathology has been demonstrated in horses with spontaneous uveitis (318).…”

Section: Chronic or Latent Infectionmentioning

confidence: 99%

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Leptospirosis

2001

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Leptospirosis is a worldwide zoonotic infection with a much greater incidence in tropical regions and has now been identified as one of the emerging infectious diseases. The epidemiology of leptospirosis has been modified by changes in animal husbandry, climate, and human behavior. Resurgent interest in leptospirosis has resulted from large outbreaks that have received significant publicity. The development of simpler, rapid assays for diagnosis has been based largely on the recognition that early initiation of antibiotic therapy is important in acute disease but also on the need for assays which can be used more widely. In this review, the complex taxonomy of leptospires, previously based on serology and recently modified by a genotypic classification, is discussed, and the clinical and epidemiological value of molecular diagnosis and typing is also evaluated

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Section: Chronic or Latent Infectionmentioning

confidence: 99%

“…The pathogenesis of equine recurrent uveitis appears to involve the production of antibodies against a leptospiral antigen which cross-react with ocular tissues (358,443). Retinal damage in horses with uveitis is related to the presence of B lymphocytes in the retina (318).…”

Section: Immune Mechanismsmentioning

confidence: 99%

Leptospirosis

2001

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“…The association of leptospires with equine recurrent uveitis (16) has been well documented, and the organism has been detected in ocular fluids by culture and PCR (31). In addition, Parma et al demonstrated by Western blotting the reactivity of several bands in extracts of equine cornea and lens with antileptospiral sera (27,28). Although there is a strong association between leptospiral infection and uveitis, the immunopathogenesis of leptospira-associated uveitis remains obscure.…”

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confidence: 99%

Cloning and Molecular Characterization of an Immunogenic LigA Protein of Leptospira interrogans

et al. 2002

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A clone expressing a novel immunoreactive leptospiral immunoglobulin-like protein A of 130 kDa (LigA) from Leptospira interrogans serovar pomona type kennewicki was isolated by screening a genomic DNA library with serum from a mare that had recently aborted due to leptospiral infection. LigA is encoded by an open reading frame of 3,675 bp, and the deduced amino acid sequence consists of a series of 90-amino-acid tandem repeats. A search of the NCBI database found that homology of the LigA repeat region was limited to an immunoglobulin-like domain of the bacterial intimin binding protein of Escherichia coli, the cell adhesion domain of Clostridium acetobutylicum, and the invasin of Yersinia pestis. Secondary structure prediction analysis indicates that LigA consists mostly of beta sheets with a few alpha-helical regions. No LigA was detectable by immunoblot analysis of lysates of the leptospires grown in vitro at 30°C or when cultures were shifted to 37°C. Strikingly, immunohistochemistry on kidney from leptospira-infected hamsters demonstrated LigA expression. These findings suggest that LigA is specifically induced only in vivo. Sera from horses, which aborted as a result of natural Leptospira infection, strongly recognize LigA. LigA is the first leptospiral protein described to have 12 tandem repeats and is also the first to be expressed only during infection.

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“…Yet, the results of the study at hand indicate a certain effect against IMMK. This could be based upon the fact that IMMK is thought to be not solely a disease of the cornea but rather a type of anterior uveitis (Parma et al 1985, Parma et al 1987, Holland et al 1993, Lucchesi and Parma 1999, Lucchesi et al 2002, Wada et al 2003. It might also explain why topically applied eye ointment does not reliably control IMMK as it does not diffuse into the deeper layers but instead only affects the superficial tissues (Bleik and Tabbara 1991, Sec-J.…”

Section: Discussionmentioning

confidence: 99%

Clinical experience and long-term outcome after subscleral insertion of a cyclosporine A drug delivery device in horses with immune-mediated keratitis

Töth¹,

Huthmann²,

Hollerrieder³

2011

PHK

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SummaryThe purpose of this study was to determine the efficacy and duration of effect of a subscleral cyclosporine A implant in horses with immune-mediated keratitis (IMMK). The study at hand describes the cases of fifteen horses with mature IMMK that were presented to the Tierklinik Hochmoor for further treatment. In theses horses, a cyclosporine A drug delivery device was placed subsclerally adjacent to the suprachoroidal space under general anesthesia. Follow-up was evaluated for up to 53 months duration after implant placement. Objective assessment showed an improvement in 66.7% of patients. Four of the patients suffered a recurrence of symptoms. This study yields promising results for the long-term treatment of IMMK in horses. Sport horses and patients suffering from frequently recurring episodes of IMMK could benefit greatly from this alternative treatment method.

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Tears and aqueous humor from horses inoculated with contain antibodies which bind to cornea

Cited by 46 publications

References 4 publications

Leptospirosis

Leptospirosis

Cloning and Molecular Characterization of an Immunogenic LigA Protein of Leptospira interrogans

Clinical experience and long-term outcome after subscleral insertion of a cyclosporine A drug delivery device in horses with immune-mediated keratitis

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