Technical aspects on production of fluid extract from Brosimum gaudichaudii Trécul roots

Abstract

Instruction:Despite the increased use of Brosimum gaudichaudii roots as raw material on medicine to treatment of vitiligo, there are not studies that showing the impact of unit operations on the quality and standardized of the extract of B. gaudichaudii. The quality of the herbal extract is essential to ensure the safety and efficacy of pharmaceutical product. Due the medical and commercial importance, this study aimed to evaluate the impact of the extraction method (ultrasound or percolation) on the quality of herbal extract and optimize the extraction of psoralen and 8-methoxypsoralen (8-MOP) from B. gaudichaudii.Materials and Methods:The extraction recovery was evaluate by high-performance liquid chromatography (C8 reverse phase column and acetonitrile: Water 45:55 and flow rate 0.6 mL/min). The extraction was performed by ultrasound-assisted extraction (UEA) or percolation using a Box-Behnken design.Results:From both chemical markers (psoralen and bergapten), the optimal conditions for the UEA were an extraction time of 25 min, the mean particle size of 100 μm, and an ethanol: Water ratio of 55:45 (v/v).Conclusion:The extraction by percolation revealed that ethanol 55% was more efficient than ethanol 80% to extract psoralen and bergapten.

Pharmacokinetics, Pharmacodynamics and Dermal Distribution of 5-Methoxypsoralen Based on a Physiologically Based Pharmacokinetic Model to Support Phytotherapy Using Brosimum gaudichaudii

“…B. gaudichaudii extract was obtained by a percolation method consisting of 55 : 45 ethanol : water (0.5 mL/min with a total volume of 10 L). It was then concentrated by a rotator evaporator at 40°C and dried by lyophilization [22]. The content of 5-MOP in the dried extract was 4.2 % (w/w).…”

“…The 5-MOP amount in the herbal extract and different tissues was identified and quantified by an HPLC diode array system (Agilent 1100 Series). The separations were performed according to a previously described methodology [22]. The following chromatographic conditions were used: a C18 reverse-phase column (VP-ODS 250 × 4.6 mm, 5 µm; Shimadzu), acetonitrile : water (55 : 45) mobile phase, a flow rate of 0.7 mL/min, and a detection wavelength of 220 nm for 5-MOP.…”

“…The following chromatographic conditions were used: a C18 reverse-phase column (VP-ODS 250 × 4.6 mm, 5 µm; Shimadzu), acetonitrile : water (55 : 45) mobile phase, a flow rate of 0.7 mL/min, and a detection wavelength of 220 nm for 5-MOP. The chromatographic methodology was previously developed and validated [22].…”

“…The samples were centrifuged at 200 × g for 15 min to separate the plasma portion. The plasma was transferred to clean tubes and stored at − 20°C until analysis by HPLC [22,26]. Animal welfare and experimental procedures were approved by the University of São Paulo (USP-13.1.914.53.8, January 13, 2014) in accordance with the U. K. Animal (Scientific Procedures) Act, 1986 and EU Directive 2010/63/EU for animal experiments.…”

Medicine bottled (garrafada): Rescue of the popular knowledge

“…Brosimum gaudichaudii, Trécul, popularly known as mama-cadela was the most frequently cited species, which was mentioned in the preparation of three medicine bottled. Studies about this species refer action to treatment of vitiligo (Filho et al, 2015) and other studies support the morphoanatomical, immunohistochemical, physiochemical, phenological, pharmacological, and toxicological aspects of the species (Martins et al, 2015;Land et al, 2017;Filho et al, 2015;Faria et al, 2015;Barbosa et al, 2014;Jacomassi et al, 2007;Cunha et al, 2008;Pozetti, 2005).…”