Technical Challenges for Specific, Sensitive Detection of Seed-Borne Bacterial Pathogens

Schaad, N. W.; Mortensen, Christian; Li, Jianqiang; Feng, Juan; Luo, Laixin; Mazzaglia, A.; Balestra, Giorgio Mariano

doi:10.1007/978-94-017-9389-6_5

Global Perspectives on the Health of Seeds and Plant Propagation Material 2014

DOI: 10.1007/978-94-017-9389-6_5

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Technical Challenges for Specific, Sensitive Detection of Seed-Borne Bacterial Pathogens

N. W. Schaad

Christian Mortensen

Jianqiang Li

et al.

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Cited by 3 publications

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“…Seed Pathology is a branch of Phytopathology which has been involved in the identification of pathogens associated with seeds since 1923 (2). Since then, detection of fungi in seeds has been gaining visibility and importance in research and certification of seeds worldwide (32).…”

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confidence: 99%

Interactive key (Lucid) for identification of fungi in vegetable seeds

et al. 2020

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Interactive keys are tools that aid research and technical work since identification of organisms has become increasingly present in the scientific and academic context. An interactive key was developed with the software Lucid v. 3.3 for the identification of eleven fungal species associated with onion, carrot, pepper and tomato seeds. It was based on a matrix composed of six features: crop, conidium, conidiophore, color of long conidiophore, color of mycelium and presence of setae, besides 21 character states. In addition, descriptions, illustrations and high-resolution photographs of the morphological characters and states were made available to aid in the correct identification of fungal species. Validation of the interactive key was performed by distinct groups of volunteers: (i) graduate students with prior knowledge and using the interactive key; (ii) undergraduate students with little prior knowledge and using the interactive key, and (iii) undergraduate students with little prior knowledge and using the conventional identification system such as the printed manuals used in seed pathology laboratories. We analyzed the time spent by each volunteer to evaluate 25 seeds infected with the fungal species in the key, as well as the percentage of success and the difficulty level for each participant. The high percentage of correct answers with the use of the interactive key and the ease of use by the volunteers confirmed its efficiency because there was an increase in the identification accuracy when compared to the conventional system. Furthermore, the rate of success and the difficulty level presented low variability within groups (i) and (ii). These results are a consequence of the interaction of the user with characteristics of the developed tool, such as high-resolution photographs, which faithfully reproduce the fungal characteristics observed in the seeds under a stereomicroscope. Thus, the interactive key presented here can aid in teaching, institutional and commercial research, inspection and certification of seeds, making diagnosis safer and more accurate. The key is available for free at https://keys.lucidcentral.org/keys/v3/seed_fungi/.

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Interactive key (Lucid) for identification of fungi in vegetable seeds

et al. 2020

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Abiotic and biotic factors affecting crop seed germination and seedling emergence: a conceptual framework

Lamichhane¹,

Debaeke²,

Steinberg

et al. 2018

Plant Soil

129

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Molecular Epidemiology ofPseudomonas syringaepv.syringaeCausing Bacterial Leaf Spot of Watermelon and Squash in Florida

et al. 2018

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From 2013 to 2014, bacterial leaf spot epidemics incited by Pseudomonas syringae pv. syringae affected an estimated 3,000 ha of watermelon and squash in Florida, and caused foliar blighting and transplant losses in severely affected fields. To investigate the diversity of the causal agent, we isolated 28 P. syringae strains from diseased plants grown in 10 Florida and Georgia counties over the course of 2 years. Strains were confirmed as P. syringae through sequence analysis of the 16S ribosomal RNA, phenotypic, and biochemical profiling; however, 20 displayed an atypical phenotype by exhibiting nonfluorescent activity on King’s medium B agar and being negative for ice-nucleating activity. Multilocus sequence analysis and BOX polymerase chain reaction revealed the presence of two haplotypes among the collected strains that grouped into two distinct clades within P. syringae phylogroup 2. Pathogenicity testing showed that watermelon, cantaloupe, and squash seedlings were susceptible to a majority of these strains. Although both haplotypes were equally virulent on cantaloupe, they differed in virulence on watermelon and squash. The distribution of one haplotype in 9 of 10 Florida and Georgia counties sampled indicated that these epidemics were associated with the recent introduction of a novel clonal P. syringae lineage throughout major watermelon production areas in Florida.

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Technical Challenges for Specific, Sensitive Detection of Seed-Borne Bacterial Pathogens

Cited by 3 publications

References 27 publications

Interactive key (Lucid) for identification of fungi in vegetable seeds

Interactive key (Lucid) for identification of fungi in vegetable seeds

Abiotic and biotic factors affecting crop seed germination and seedling emergence: a conceptual framework

Molecular Epidemiology ofPseudomonas syringaepv.syringaeCausing Bacterial Leaf Spot of Watermelon and Squash in Florida

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