Technical note: A linear model for predicting δ<sup>13</sup>C<sub>protein</sub>

Pestle, William J.; Hübbe, Mark; Smith, Erin K.; Stevenson, Joseph Martin

doi:10.1002/ajpa.22743

Cited by 41 publications

(16 citation statements)

References 48 publications

(114 reference statements)

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“…The two highlighted combinations (3:1 C 3 :C 4 protein and energy versus 1:3 C 3 :C 4 protein and energy) in Table 6 represent the best approximations of the end-member dietary combinations seen in the Larache individuals (3,802 and 360, respectively), with the remaining individuals falling somewhere between these extremes. Similar results for modeled dietary protein (displayed in Table 3) are obtained using the formula proposed by Pestle et al (2015). These modeled solutions are testament to the fact that some individuals (with lower d 13 C collagen and d 13 C apatite values) were subsisting on C 3 -foddered terrestrial fauna and C 3 carbohydrates, similar to individuals from the Loa Basin , central Chile (Falabella et al, 2007), and central western Argentina (Gil et al, 2006), whereas others were likely consuming a large portion of C 4 -foddered terrestrial fauna and C 4 plants, much more like individuals from coastal and highland Peru (Finucane et al, 2006;Kellner and Schoeninger, 2008).…”

Section: Stable Carbon and Nitrogen Isotope Datasupporting

confidence: 74%

Tiwanaku influence and social inequality: A bioarchaeological, biogeochemical, and contextual analysis of the Larache cemetery, San Pedro de Atacama, Northern Chile

Torres‐Rouff

Knudson

Pestle

et al. 2015

American J Phys Anthropol

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Section: Stable Carbon and Nitrogen Isotope Datasupporting

confidence: 74%

Tiwanaku influence and social inequality: A bioarchaeological, biogeochemical, and contextual analysis of the Larache cemetery, San Pedro de Atacama, Northern Chile

Torres‐Rouff

Knudson

Pestle

et al. 2015

American J Phys Anthropol

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“…The estimated fractional contribution of dietary protein to collagen varied by species, about 63% for the two rat studies, 80% for mice, and 83% for pigs. Pestle et al [20] derived a similar regression model to predict the δ 13 C values of dietary protein from isotopic measurements of apatite and collagen. They reported that their model residuals differed significantly between pigs (n = 15) and rodents (n = 21), implying differences in how these organisms processed protein and energy sources into collagen.…”

Section: Protein Content Controlled Metabolic Routingmentioning

confidence: 99%

Dietary protein content and tissue type control ¹³C discrimination in mammals: an analytical approach

Hobbie

2017

Rapid Comm Mass Spectrometry

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RATIONALE: Carbon isotope measurements on collagen and other tissues are used in modern and paleodiet studies, and relating these patterns to the dietary protein fraction (f dp ) and to δ 13 C values of dietary protein and energy (lipids and carbohydrates) remains important for determining diets. METHODS: Carbon fluxes from dietary protein to tissues in feeding studies of mice, rats, and pigs were estimated to test how tissue δ 13 C values related to the δ 13 C values of dietary protein, dietary energy, f dp , or log e f dp . Values of f dp , protein δ 13 C, and energy δ 13 C were used in stepwise regressions that estimated the metabolic routing of dietary protein and the 13 C enrichment of tissues relative to dietary protein. RESULTS:The 13 C enrichment of rat collagen and mouse muscle relative to dietary protein depended on log e f dp and the 13 C enrichment of dietary protein on energy: δ 13 C collagenÀprotein ¼ 0:160Âlog e f dp Âδ 13 C proteinÀenergy þ 5:34‰ δ 13 C muscleÀprotein ¼ 0:082Âlog e f dp Â δ 13 C proteinÀenergy þ 2:92‰The collagen (f cp ) or muscle (f mp ) fractions derived from dietary protein were estimated as:The coefficients of log e f dp reflected the higher proportion of non-essential amino acids in collagen (79%) than in muscle (61%). In rats, dietary protein contributed from 66% (liver) to 85% (hair) whereas, in mice, dietary protein contributed from 76% (liver) to 85% (body protein). In pigs, collagen derived primarily (83 ± 5%) from dietary protein. CONCLUSIONS: These equations provided analytical frameworks to interpret dietary patterns from isotopic measurements. Metabolic routing and 13 C enrichment varied with f dp and with the tissue amino acid composition. Thus, f dp and tissue amino acid compositions were key parameters to assess dietary sources from isotopic values. Incorporating these insights will improve our ability to interpret the isotopic paleorecord and modern diet studies.

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“…The present work adds 19 individuals to a previously published sample of 12 individuals from Ancachi/Quillagua (Pestle, Torres‐Rouff, Gallardo Ibanez, Andrea Cabello, & Smith, ). Ultimately, we consider both the internal variability and structure of the paleodiet of these individuals and then position them against a larger regional sample of nearly 200 previously analyzed individuals from the Formative Period (Pestle, ; Pestle, Torres‐Rouff, Hubbe, Santana, Pimentel, Gallardo, & Knudson, ; Pestle, Torres‐Rouff, Gallardo, Ballester, & Clarot, ).…”

Section: Methodsmentioning

confidence: 99%

An isotopic study of dietary diversity in formative period Ancachi/Quillagua, Atacama Desert, northern Chile

Pinder

Gallardo

Cabello

et al. 2019

American J Phys Anthropol

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Objectives: To characterize the paleodiet of individuals from Formative Period (1500 B.C.-A.D. 400) Atacama Desert sites of Ancachi and Quillagua as a means of understanding the dietary and cultural impacts of regional systems of exchange. Materials and methods: Thirty-one bone samples recovered from the cemetery of Ancachi (02QU175) and in/around the nearby town of Quillagua were the subject of carbon and nitrogen stable isotope analysis of bone collagen and hydroxyapatite and multisource mixture modeling (FRUITS, food reconstruction using isotopic transferred signals) of paleodiet. These individuals were compared with nearly 200 other Formative Period individuals from throughout the region to identify differences in dietary behaviors. Results: 80.6% (25/31) of the samples yielded sufficient well-preserved collagen and were included in the multisource mixture model. The FRUITS model, which compared individuals with a robust database of available foods from the region, identified a wide diversity of diets in the Ancachi/Quillagua area (including both coastal and interior individuals), and, most notably, thirteen individuals who consumed an average of 11.2 ± 1.9% terrestrial animals, 19.8 ± 1.9% legumes, and 22.5 ± 3.1% marine fauna, a balanced pattern of protein consumption distinct from both the coastal and inland individuals in our larger regional sample. Conclusions:The combination of stable isotope analysis and multisource mixture modeling permitted the characterization of dietary behavior of 25 individuals from nodal sites in the Atacama Desert, thus enhancing our understanding of the economic and social relationships that bound together Formative Period sites, populations, and individuals in this hyperarid region.

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Technical note: A linear model for predicting δ¹³C_protein

Cited by 41 publications

References 48 publications

Tiwanaku influence and social inequality: A bioarchaeological, biogeochemical, and contextual analysis of the Larache cemetery, San Pedro de Atacama, Northern Chile

Tiwanaku influence and social inequality: A bioarchaeological, biogeochemical, and contextual analysis of the Larache cemetery, San Pedro de Atacama, Northern Chile

Dietary protein content and tissue type control ¹³C discrimination in mammals: an analytical approach

An isotopic study of dietary diversity in formative period Ancachi/Quillagua, Atacama Desert, northern Chile

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Technical note: A linear model for predicting δ13Cprotein

Cited by 41 publications

References 48 publications

Tiwanaku influence and social inequality: A bioarchaeological, biogeochemical, and contextual analysis of the Larache cemetery, San Pedro de Atacama, Northern Chile

Tiwanaku influence and social inequality: A bioarchaeological, biogeochemical, and contextual analysis of the Larache cemetery, San Pedro de Atacama, Northern Chile

Dietary protein content and tissue type control 13C discrimination in mammals: an analytical approach

An isotopic study of dietary diversity in formative period Ancachi/Quillagua, Atacama Desert, northern Chile

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Technical note: A linear model for predicting δ¹³C_protein

Dietary protein content and tissue type control ¹³C discrimination in mammals: an analytical approach