Technical note: A simple rumen collection device for calves: An adaptation of a manual rumen drenching system

Klopp, Rebecca N.; Oconitrillo, Maria Jose; Sackett, A.; Hill, T.M.; Schlotterbeck, R.L.; Lascano, G.J.

doi:10.3168/jds.2017-14201

Cited by 8 publications

(6 citation statements)

References 7 publications

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“…Rumen content (70 mL/animal, liquid part) was collected using an oral stomach tube from each animal before morning grazing and snap-frozen in liquid nitrogen and then stored at − 80 °C until use. Polyvinyl chloride oral tube (length = 125 cm) with small side holes (7 mm in diameter) located at the insertion end was used for young animals [ 57 ]. The length of the tube to be inserted was measured as the distance from the tip of the calf’s nose to the point of its elbow behind the front leg and marked on the tube with a piece of tape i.e., approximately 45 cm.…”

Section: Methodsmentioning

confidence: 99%

Age-dependent variations in rumen bacterial community of Mongolian cattle from weaning to adulthood

et al. 2022

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Background Rumen microbes play an important role in ruminant energy supply and animal performance. Previous studies showed that the rumen microbiome of Mongolian cattle has adapted to degrade the rough forage to provide sufficient energy to tolerate the harsh desert ecological conditions. However, little is known about the succession of rumen microbes in different developmental stages of post-weaning Mongolian cattle. Methods Here, we examined the succession of the rumen microbial composition and structure of 15 post-weaning Mongolian cattle at three developmental stages i.e., 5 months (RM05), 18 months (RM18) and, 36 months (RM36) by using the 16S rRNA gene sequencing method. Results We did not find any age-dependent variations in the ruminal concentrations of any volatile fatty acid (VFA) of Mongolian cattle. The diversity of the rumen bacterial community was significantly lower in RM05 group, which reached to stability with age. Bacteroidetes and Firmicutes were the two dominant phyla among all age groups. Phylum Actinobacteria was significantly higher in RM05 group, phyla Spirochaetes, and Tenericutes were highly abundant in RM18 group, and phyla Proteobacteria and Epsilonbacteraeota were enriched in RM36 group. Genera Prevotella_1, Bacteroides, and Bifidobacterium were abundant in RM05 group. The short chain fatty acid (SCFA) producing bacteria Rikenellaceae_RC9_gut_group showed high abundance in RM18 group and fiber degrading genus Alloprevotella was highly abundant in RM36 group. Random forest analysis identified Alloprevotella, Ileibacterium, and Helicobacter as important age discriminatory genera. In particular, the genera Ruminococcaceae_UCG-005, Bacteroides, Saccharofermentans, and Fibrobacter in RM05, genera [Eubacterium] coprostanoligenes_group, Erysipelotrichaceae_UCG-004, Helicobacter, Saccharofermentans, Papillibacter, and Turicibacter in RM18, and genera Rikenellaceae_RC9_gut_group, Lachnospiraceae_AC2044_group, and Papillibacter in RM36 showed the top interactions values in the intra-group interaction network. Conclusions The results showed that rumen microbiota of Mongolian cattle reached to stability and maturity with age after weaning. This study provides some theoretical evidence about the importance of functional specific rumen bacteria in different age groups. Further studies are needed to determine their actual roles and interactions with the host.

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Section: Methodsmentioning

confidence: 99%

Age-dependent variations in rumen bacterial community of Mongolian cattle from weaning to adulthood

et al. 2022

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“…Ruminal fluid samples were collected from all calves at 3 time-points relative to weaning: 7 days pre-weaning (−7 d), at weaning (0 d), and 7 days post-weaning (7 d). Two people restrained each calf with minimal force and samples were collected via an oro-ruminal probe attached to a manual suction pump, 1–2 h post-feeding using the technique described by Klopp et al [ 20 ]. Ruminal fluid pH was measured on-site with a portable device (Hanna ® Instruments, Woonsocket, RI, USA).…”

Section: Methodsmentioning

confidence: 99%

Phenotypical Variation of Ruminal Volatile Fatty Acids and pH during the Peri-Weaning Period in Holstein Calves and Factors Affecting Them

Kazana

Siachos

Panousis

et al. 2022

Animals

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Two hundred and forty-three clinically healthy Holstein calves from eight commercial dairy farms were used to: (a) describe the evolutionary course of ruminal VFA concentration and pH during the peri-weaning period and (b) assess management factors affecting their phenotypical variation of these parameters. Management practices were recorded individually for each calf as these were not fixed within farms. Samples of ruminal fluid were collected at −7 d, 0 d, and 7 d relative to weaning. Gas chromatography was used to measure ruminal VFAs, and pH was measured on site. Linear mixed models for repeated measurements were used to assess the effects of management factors and their interactions. A large among-calves phenotypical variability was observed. Estimated marginal means showed that concentrations of acetate, butyrate, and total VFAs (but not of propionate) significantly decreased, while acetate propionate increased, from −7 d to 7 d. Age at weaning and body weight at −7 d were positively associated with total and several individual VFA concentrations. Group housing and late forage feeding pre-weaning were associated with higher VFA concentrations; the same factors, as well as step-weaning, were associated with pH values around 6. Feeding 7–8 L of milk replacer daily did not preclude a smooth transition, irrespective of weaning method.

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“…Ruminal fluid was collected using a locally manufactured device consisting of a short hose attached to a suction pump as previously described (Klopp et al., 2018). The physical and laboratory examination were immediately performed to the samples in order to avoid alteration in the ruminal fluid properties as a result of exposure to the air.…”

Section: Methodsmentioning

confidence: 99%

Concentration of 2,3 bisphosphoglycerate in cattle affected with acute ruminal acidosis

Moosa

Al-Obaidi

Mustafa

et al. 2021

Veterinary Medicine & Sci

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The main objective of the study conducted here was to estimate the concentration of 2,3‐Bisphosphoglycerate (2,3‐BPG), 1,3‐Bisphosphoglycerate (1,3‐BPG), bisphospho‐glycerate mutase (BPGM) and 3‐phosphoglycerate (3PG) in cattle clinically diagnosed with acute ruminal acidosis. A secondary objective was to examine the physical and chemical characteristics of the ruminal fluid in affected cattle. A total of 20 cattle clinically diagnosed with acute ruminal acidosis and eight clinically normal cattle were included in this study. The results showed that decrease of ruminal pH changed the ruminal fluid colour, odour and consistency, as well as decreased the sedimentation time, increased the methylene blue reduction time, and decreased ruminal microflora motility. The study indicated that the concentration of 2,3‐BPG, BPGM and BPGP decreased with the decrease of ruminal pH, while 3‐PG concentration was not affected with the decrease of ruminal pH. In conclusion, 2,3‐BPG could play a role in the pathogenesis of ruminal acidosis, and thus, the intravenous administration of sodium bicarbonate is important, particularly in severe cases, to correct any systemic acidosis that can decrease 2,3‐BPG concentration and results in tissue hypoxia.

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Technical note: A simple rumen collection device for calves: An adaptation of a manual rumen drenching system

Cited by 8 publications

References 7 publications

Age-dependent variations in rumen bacterial community of Mongolian cattle from weaning to adulthood

Age-dependent variations in rumen bacterial community of Mongolian cattle from weaning to adulthood

Phenotypical Variation of Ruminal Volatile Fatty Acids and pH during the Peri-Weaning Period in Holstein Calves and Factors Affecting Them

Concentration of 2,3 bisphosphoglycerate in cattle affected with acute ruminal acidosis

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