Technical Note Polymorphic microsatellite loci from two enriched genomic libraries for the genetic analysis of the miiuy croaker, Miichthys miiuy (Sciaenidae)

Wang, R. X.; Xu, T. J.; Sun, Yuena; Guo, He

doi:10.4238/vol9-2gmr806

Cited by 10 publications

(9 citation statements)

References 9 publications

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“…Massive parallel sequencing in recent times has been employed for the development of SSR markers in many organisms to reduce the cost, labour and time required (Abdelkrim et al 2009;Csencsics et al 2010;Kircher and Kelso 2010;Yu et al 2011). Various microsatellite development protocols based on partial genomic library enriched for microsatellite are available in the literature Pardo et al 2007;Abdelkrim et al 2009;Wang et al 2010). However, marker development following Sanger sequencing has shown to be more effective (Malausa et al 2011).…”

Section: Ssr Marker Developmentmentioning

confidence: 97%

Preliminary genetic linkage map of Indian major carp, Labeo rohita (Hamilton 1822) based on microsatellite markers

Sahoo

Patel

Sahu

et al. 2015

J Genet

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Linkage map with wide marker coverage is an essential resource for genetic improvement study for any species. Sex-averaged genetic linkage map of Labeo rohita, popularly known as 'rohu', widely cultured in the Indian subcontinent, was developed by placing 68 microsatellite markers generated by a simplified method. The parents and their F1 progeny (92 individuals) were used as segregating populations. The genetic linkage map spans a sex-averaged total length of 1462.2 cM, in 25 linkage groups. The genome length of rohu was estimated to be 3087.9 cM. This genetic linkage map may facilitate systematic searches of the genome to identify genes associated with commercially important characters and marker-assisted selection programmes of this species.

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Section: Ssr Marker Developmentmentioning

confidence: 97%

Preliminary genetic linkage map of Indian major carp, Labeo rohita (Hamilton 1822) based on microsatellite markers

Sahoo

Patel

Sahu

et al. 2015

J Genet

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“…Ten polymorphic SSR loci from our library were used in this study (Wang et al, 2010;Xu et al, 2011c). PCR was carried out in a 15-μL reaction mixture containing 9.9 µL sterile water, 1.5 µL 10X PCR buffer (containing 1.5 mM Mg 2+ ), 1.2 µL 2.5 mM dNTPs, 0.6 µL forward primer, 0.6 µL reverse primer, 1 µL diluted DNA template, and 0.1 µL 5 U/µL Taq DNA polymerase (Tiangen).…”

Section: Sample Collection Dna Extraction and Ssr Analysismentioning

confidence: 99%

Genetic diversity and population genetic structure of the miiuy croaker, Miichthys miiuy, in the East China Sea by microsatellite markers

Sun

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Genetic diversity and patterns of population structure of the miiuy croaker were investigated using SSR markers. A set of 10 microsatellite loci revealed 40 alleles; the number of alleles varied from 2 to 10 for each marker. A relatively high level of genetic variability was observed between miiuy croaker individuals. Genetic diversity was relatively high within populations with corresponding high average gene flow. There were genealogical branches or clusters corresponding to sampling localities according to the UPGMA tree and principal component analysis. Knowledge of the genetic diversity and population structure will be crucial for establishing appropriate fishery management stocks for this species.

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“…Total genomic DNA was extracted using traditional phenol-chloroform extraction protocols, as described by Liao et al (2007). Isolation of microsatellite loci from an enriched library was performed as recommended by Wang et al (2010), by using 5ꞌ-biotin-labeled microsatellite oligoprobes [AC] 15 and streptavidin MagneSphere paramagnetic particles (Promega). Capture fragments were ligated to pGEM-T Easy clone vector (Promega) and transformed into JM109 competent cells (Promega).…”

Section: Methodsmentioning

confidence: 99%

Development of 18 microsatellite loci for the freshwater snail Bellamya aeruginosa (Mollusca, Gastropoda)

Bx²,

Zhu³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Although it is a major freshwater gastropod species, genetic diversity of Bellamya aeruginosa was completely unknown. Eighteen microsatellite loci were isolated and characterized from (AC) 15 -enriched genomic libraries of the freshwater snail B. aeruginosa. Most of the 18 loci were successfully amplified and high polymorphic information content values were found, ranging from 0.244 to 0.792 (mean 0.541). The number of alleles per locus ranged from 5 to 13 (mean 8.8), the expected heterozygosity varied from 0.347 to 0.950 (mean 0.815) and the observed heterozygosity varied from 0.087 to 0.782 (mean 0.431). Eight loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni's correction and no significant genotypic linkage disequilibrium was detected between most locus pairs, except for TXH79-TXH97 and TXH113-TXH121. These 18 polymorphic microsatellite loci should be useful for population genetics analysis and species identification of Bellamya.

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Technical Note Polymorphic microsatellite loci from two enriched genomic libraries for the genetic analysis of the miiuy croaker, Miichthys miiuy (Sciaenidae)

Cited by 10 publications

References 9 publications

Preliminary genetic linkage map of Indian major carp, Labeo rohita (Hamilton 1822) based on microsatellite markers

Preliminary genetic linkage map of Indian major carp, Labeo rohita (Hamilton 1822) based on microsatellite markers

Genetic diversity and population genetic structure of the miiuy croaker, Miichthys miiuy, in the East China Sea by microsatellite markers

Development of 18 microsatellite loci for the freshwater snail Bellamya aeruginosa (Mollusca, Gastropoda)

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