Various food-processing techniques like malting, roasting, soaking, germination, fermentation, etc. are known to enhance nutritive value and acceptability of different food products. The present study deals with the biochemical analysis and acceptability tests of soya flour rabadi, which was made by fermentation before and after cooking at different time intervals. There was a trend towards increase in moisture, ash, total carbohydrate, calcium, total soluble sugars, reducing sugars, non-reducing sugars, amylase, glutamate oxaloacetate transaminase enzymatic activities and titrable acidity, whereras a trend towards decrease was found in crude protein, fat, fibre, energy, starch, total phenols and glutamate pyruvate transaminase enzyme activity. Major biochemical changes occurred after 8 h fermentation. In sensory evaluation also, samples fermented for 4 and 8 h after cooking got higher scores than all other samples.