1988
DOI: 10.1179/000705988798270820
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Technical note: Staining procedures for characterising biofilms in corrosion investigations

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Cited by 28 publications
(12 citation statements)
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“…The pits were located in a membrane of cuprous oxide or copper and beneath that were crystals of cuprous oxide extending from the perforations outward in a hemispherical fashion. Further examination of these tube bores has shown that a biofilm can always be lifted from the surface and stained using the periodic acidSchiff stain [234], showing the presence of polysaccharides (by-products of bacteria), which form in copious quantities when biofilms develop. This type of pitting resembles the classical type 3 of pitting [40].…”
Section: E3 Microbial Corrosionmentioning
confidence: 99%
“…The pits were located in a membrane of cuprous oxide or copper and beneath that were crystals of cuprous oxide extending from the perforations outward in a hemispherical fashion. Further examination of these tube bores has shown that a biofilm can always be lifted from the surface and stained using the periodic acidSchiff stain [234], showing the presence of polysaccharides (by-products of bacteria), which form in copious quantities when biofilms develop. This type of pitting resembles the classical type 3 of pitting [40].…”
Section: E3 Microbial Corrosionmentioning
confidence: 99%
“…Microscopy. Using light microscopy and proper staining, investigators [31,32] have demonstrated a relationship between an unusual variety of copper pitting corrosion and gelatinous, polysaccharide-containing biofilms. Epifluorescence microscopy techniques have been developed for the identification of specific bacteria in biofilms [33,34].…”
Section: B12 Biochemicalmentioning
confidence: 99%
“…É evidenciada a formação de pites e exibem-se as ranhuras devido ao polimento da amostra na ocasião tratamento inicial da mesma. Provavelmente o meio possui potencial para a promoção de corrosão localizada, como encontrado por vários autores [1,4,8,12,16,20,35].…”
Section: Figuraunclassified
“…Por outro lado, tem-se que os microorganismos, principalmente, as bactérias se encontram em qualquer meio. A bactéria Salmonella, bactéria muito comum de ser encontrada no meio ambiente tem seu hábitat freqüente o trato intestinal de répteis, mamíferos e peixes [4], o que a torna uma bactéria bastante dispersa no ambiente. Segundo Videla [1], as quatro características anatômicas e fisiológicas dos microorganismos mais relevantes aos estudos de biocorrosão, biofouling e biodeterioração são a grande velocidade de reprodução; alta relação superfície/volume, alta atividade e flexibilidade metabólica de troca com o meio e distribuição uniforme no ambiente.…”
Section: Introductionunclassified