Technical note: Viability and motility of vitrified/thawed primordial germ cell isolated from common carp (Cyprinus carpio) somite embryos1

Kawakami, Yutaka; Saito, Taiju; Fujimoto, Takafumi; Goto-Kazeto, Rie; Takahashi, Eisuke; Adachi, Souichi; Arai, Katsutoshi; Yamaha, Etsuro

doi:10.2527/jas.2011-4329

Cited by 11 publications

(1 citation statement)

References 16 publications

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“…Guan, Rawson & Zhang ), eggs (e.g. Urbanyi, Magyary, Horvath, Baranyai & Dinnyes ), embryos (e.g., Edashige, Valdez, Hara, Saida, Seki & Kasai ) and primordial germ cells (e.g., Kawakami, Saito, Fujimoto, Goto‐Kazeto, Takahashi, Adachi, Arai & Yamaha ). Vitrification has been demonstrated to be a useful tool for the cryopreservation of primordial germ cells (Kawakami, Ishihara, Saito, Fujimoto, Adachi, Arai & Yamaha ).…”

Section: Introductionmentioning

confidence: 99%

Vitrification of sperm from marine fish: effect on motility and membrane integrity

et al. 2013

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Our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. The objectives were to: 1) estimate acute toxicity of cryoprotectants over a range of concentrations; 2) evaluate the properties of vitrification solutions (VS); 3) evaluate different thawing solutions, and 4) evaluate sperm quality after thawing by examination of motility and membrane integrity. Sperm were collected from red snapper (Lutjanus campechanus), spotted seatrout (Cynoscion nebulosus), and red drum (Sciaenops ocellatus). A total of 29 combinations of cryoprotectants were evaluated for toxicity and glass formation. Samples were loaded onto 10-µL polystyrene loops and plunged into liquid nitrogen. There was a significant difference (P < 0.05) in post-thaw motility among VS and among species when using the same VS. The sperm in VS of 15% DMSO + 15% ethylene glycol + 10% glycerol + 1% X-1000™ + 1% Z-1000™ had an average post-thaw motility of 58% and membrane integrity of 19% for spotted seatrout, 38% and 9% for red snapper, and 30% and 19% for red drum. Adaptations by marine fish to high osmotic pressures could explain the survival in the high cryoprotectant concentrations. Vitrification offers an alternative to conventional cryopreservation.

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Section: Introductionmentioning

confidence: 99%

Vitrification of sperm from marine fish: effect on motility and membrane integrity

et al. 2013

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Current and Future Assisted Reproductive Technologies for Fish Species

Weber

Lee

2013

Current and Future Reproductive Technologies and World Food Production

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The Food and Agriculture Organization of the United Nations (FAO) estimates that in 2012 aquaculture production of fish will meet or exceed that of the capture fisheries for the first time. Thus, we have just turned the corner from a predominantly hunting gathering approach to meeting our nutritional needs from fish, to a farming approach. In 2012, 327 finfish species and five hybrids were covered by FAO aquaculture statistics, although farming of carps, tilapias, salmonids, and catfishes account for most of food-fish production from aquaculture. Although for most major species at least part of production is based on what might be considered domesticated animals, only limited production in most species is based on farming of improved lines of fish or is fully independent of wild seedstock. Consistent with the infancy of most aquaculture industries, much of the development and implementation of reproductive technologies over the past 100 years has been directed at completion of the life cycle in captivity in order to increase seed production and begin the process of domestication. The selection of species to farm and the emphasis of selective breeding must also take into account other ways to modify performance of an animal. Reproductive technologies have also been developed and implemented to affect many performance traits among fishes. Examples include technologies to control gender, alter time of sexual maturation, and induce sterilization. These technologies help take advantage of sexually dimorphic growth, overcome problems with growth performance and flesh quality associated with sexual maturation, and genetic containment. Reproductive technologies developed to advance aquaculture and how these technologies have been implemented to advance various sectors of the aquaculture industry are discussed. Finally, we will present some thoughts regarding future directions for reproductive technologies and their applications in finfish aquaculture.

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Recent Advances and Prospects in Germplasm Preservation of Rare and Endangered Species

Comizzoli

Holt

2014

Advances in Experimental Medicine and Biology

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Fertility preservation strategies using cryopreservation have enormous potential for helping sustain and protect rare and endangered species, especially to assist managing or 'rescuing' the genomes of genetically valuable individuals. However, wide-scale applications are still limited by significant physiological variations among species and a sheer lack of fundamental knowledge about basic reproductive traits as well as in germplasm cryobiology. Cryo-studies have been conducted in more species (mainly vertebrates) in the recent years but a vast majority still remains un-studied. Semen cryopreservation represents the most extensive effort with live births reported in more and more species after artificial insemination. Oocyte freezing remains challenging and unsuccessful in wild species and will require more research before becoming a standard procedure. As an alternative to fully grown gametes, gonadal tissue preservation has become a promising option in vertebrates. Yet, more fertility preservation options are necessary to save species so a change in strategy might be required. It is worthwhile thinking beyond systematic characterizations and considering the application of cutting edge approaches to universally preserve the fertility of a vast array of species.

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Technical note: Viability and motility of vitrified/thawed primordial germ cell isolated from common carp (Cyprinus carpio) somite embryos1

Cited by 11 publications

References 16 publications

Vitrification of sperm from marine fish: effect on motility and membrane integrity

Vitrification of sperm from marine fish: effect on motility and membrane integrity

Current and Future Assisted Reproductive Technologies for Fish Species

Recent Advances and Prospects in Germplasm Preservation of Rare and Endangered Species

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