“…The microfluidics-based devices that are being developed for large-scale automated combinatorial studies of crystallization conditions use smaller and smaller volumes of protein, limiting the size of crystals that may be grown in them (Shim et al, 2007). A few X-ray beamlines exist, and more are being developed, with sufficiently powerful <10 mm diameter beams to produce observable diffraction from <10 mm crystals (Moukhametzianov et al, 2008;Sanishvili et al, 2008;Bilderback et al, 2006;Dierker, 2008). Hence, it appears that the single-shot-per-crystal regime is of more than theoretical interest.…”