Technical Review: <i>In Situ</i> Hybridization

Jensen, Ellen C.

doi:10.1002/ar.22944

Cited by 126 publications

(106 citation statements)

References 23 publications

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“…Oligonucleotide hybridization comprises a fundamental basis for sequence‐specific nucleic acid detection strategies ranging from DNA and RNA detection blots and gene arrays to fluorescence in situ hybridization and fluorescence resonance energy transfer probes . Nucleic acid hybridization is also an essential feature of modern gene‐targeting techniques such as antisense gene therapy and CRISPR/Cas9 .…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O⁶‐Alkylguanine Adducts

Dahlmann

Berger

Kung

et al. 2018

Helvetica Chimica Acta

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Chemical alkylation of DNA produces potentially toxic and mutagenic damage such as O6‐alkylguanine (O6‐alkylG) adducts. Non‐natural nucleoside analogues that pair with DNA adducts provide a potential basis for studying damaged DNA. Herein, we evaluated the base pairing properties of elongated nucleoside analogues containing napthalene‐derived tricyclic nucleobases as DNA adduct‐pairing nucleoside analogues in DNA hybridization probes. DNA duplex melting studies revealed that the elongated nucleoside analogs formed more stable base pairs opposite O6‐alkylG than G and were better able to distinguish between G, O6‐alkylG, and an abasic site than any previously described nucleoside analogue. DNA duplexes containing an elongated base analogue exhibited different fluorescence intensities when paired opposite O6‐alkylG vs. G or abasic sites. Their selectivity for stabilizing alkylated DNA make the elongated hydrophobic base analogues improved candidates for incorporating into DNA hybridization probes targeting O6‐alkylG.

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Section: Introductionmentioning

confidence: 99%

Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O⁶‐Alkylguanine Adducts

Dahlmann

Berger

Kung

et al. 2018

Helvetica Chimica Acta

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“…FISH allows the detection of viral genomic sequences with high specificity; however, detection of low-titer viral infections and new viruses are not possible. [51]…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Viral Detection: Past, Present, and Future

et al. 2019

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Viruses are essentially composed of a nucleic acid (segmented or not, DNA, or RNA) and a protein coat. Despite their simplicity, these small pathogens are responsible for significant economic and humanitarian losses that have had dramatic consequences in the course of human history. Since their discovery, scientists have developed different strategies to efficiently detect viruses, using all possible viral features. Viruses shape, proteins, and nucleic acid are used in viral detection. In this review, the development of these techniques, especially for plant and mammalian viruses, their strengths and weaknesses as well as the latest cutting‐edge technologies that may be playing important roles in the years to come are described.

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“…In case of northern blot, tissue digest is used as starting material while in in situ hybridization histological section is used. Regardless of using the direct hybridization, the identifications using signal hybridization are most efficiently obtained after fungi growth or its biological amplification (Jensen 2014). The main advantage of this technology is the maximum use of the short supply tissue such as clinical biopsies, and cultured cells.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Recent advances in molecular techniques for the identification of phytopathogenic fungi – a mini review

Aslam

Tahir

Aslam

et al. 2017

Journal of Plant Interactions

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At present, 1.5 million species of fungi are estimated. Among these less than 5% have been described. Many fungal species cause disease in plants. These diseases cause major economic and production losses in the agricultural industry worldwide. Monitoring plant health and detecting the pathogen early are essential to reduce the disease spread, and facilitate effective management practices. DNA-based methods now provide essential tools for accurate plant disease diagnosis. Recently, effective amplification platforms, probe development, various quantitative PCR, DNA barcoding and RNA-Seq-based next-generation sequencing have revolutionized the research in fungal detection field, and differentiation area. Although the molecular diagnostics techniques have grown extensively over the last couple of decades but still there is a long way to go in the development and application of molecular diagnostics to assist the plant disease diagnosticians. Finally, molecular diagnostic techniques used in plant disease diagnostic clinics need to be robust, reliable, inexpensive and easy to be used that they can compete with, and complement traditional techniques. Challenge now remains residue with the researchers to develop the practical techniques used for diagnostic setting. Examples of the recent advancement in the molecular techniques for diagnosing the fungi causing plant disease are discussed in the review.

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Technical Review: In Situ Hybridization

Cited by 126 publications

References 23 publications

Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O⁶‐Alkylguanine Adducts

Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O⁶‐Alkylguanine Adducts

Viral Detection: Past, Present, and Future

Recent advances in molecular techniques for the identification of phytopathogenic fungi – a mini review

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Technical Review: In Situ Hybridization

Cited by 126 publications

References 23 publications

Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O6‐Alkylguanine Adducts

Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O6‐Alkylguanine Adducts

Viral Detection: Past, Present, and Future

Recent advances in molecular techniques for the identification of phytopathogenic fungi – a mini review

Contact Info

Product

Resources

About

Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O⁶‐Alkylguanine Adducts

Fluorescent Nucleobase Analogues with Extended Pi Surfaces Stabilize DNA Duplexes Containing O⁶‐Alkylguanine Adducts