Technique and Clinical Relevance of a Cytochemical Bioassay for Gastrin-like Activity

Hoile, Ronald W.

doi:10.1016/b978-0-407-00344-6.50014-1

Search citation statements

Order By: Relevance

Paper Sections

Select...

The First Bioassay (Acth)1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2003

2004

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To overcome this, cytochemical section bioassays were developed based on the existing assays for ACTH, 44 LH, 45 TSH 46,47 and gastrin. 48 Later a new bioassay was developed for measuring trilostane in human plasma. 49 These developments meant that multiple samples could be measured within a single assay and highlighted the fact that sections prepared in the correct way could still be shown to be responsive to hormonal signals.…”

Section: The First Bioassay (Acth)mentioning

confidence: 99%

Cytochemical bioassays: every dog has its day

Loveridge

2003

Cell Biochemistry & Function

View full text Add to dashboard Cite

Section: The First Bioassay (Acth)mentioning

confidence: 99%

Cytochemical bioassays: every dog has its day

Loveridge

2003

Cell Biochemistry & Function

View full text Add to dashboard Cite

Hypergravity decreases carbonic anhydrase‐reactivity in inner ear maculae of fish

2004

View full text Add to dashboard Cite

Previous investigations revealed that fish inner ear otolith growth depends on the amplitude and the direction of gravity. Both otolith total size, otolith bilateral size-asymmetry and the total and bilateral calcium-incorporation are also affected by gravity. Hypergravity, e.g., slows down otolith growth and diminishes bilateral otolith asymmetry as compared to 1 g control specimens raised in parallel. Since the enzyme carbonic anhydrase (CA) plays a prominent role in otolithic calcification, the reactivity of inner ear CA during otolith growth under hypergravity was investigated. CA-reactivity was demonstrated histochemically and densitometrically on sections of inner ear maculae of larval cichlid fish (Oreochromis mossambicus), that were kept for 6 hrs in a 3 g hypergravity centrifuge. The total unilateral macular CA-reactivity and the bilateral difference in CA between the left and the right maculae were significantly lower in 3 g animals than in 1g controls. The result is in complete agreement with previous studies indicating that a regulatory mechanism, which adjusts otolith size and asymmetry towards the gravity vector, acts via activation/deactivation of macular CA.

show abstract

Technique and Clinical Relevance of a Cytochemical Bioassay for Gastrin-like Activity

Cited by 2 publications

References 36 publications

Cytochemical bioassays: every dog has its day

Cytochemical bioassays: every dog has its day

Hypergravity decreases carbonic anhydrase‐reactivity in inner ear maculae of fish

Contact Info

Product

Resources

About