1991
DOI: 10.1002/lsm.1900110613
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Technique for cellular microsurgery using the 193‐nm excimer laser

Abstract: A new cell surgery technique has been developed to produce well-defined alterations in cells and tissue without detectable heating and/or other structural damage in the surroundings. The technique involves the use of an argon fluoride excimer laser, in the deep ultraviolet (UV) region of the spectrum at 193 nm, which is guided through a glass pipette filled with a positive air pressure. To demonstrate the method, holes were drilled in the zona pellucida of mouse oocytes. The diameter of the drilled hole was de… Show more

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Cited by 71 publications
(31 citation statements)
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“…Laser-assisted zona drilling was first reported in 1991 by two groups led by Tadir [53] and Palankar [54]. Laser presents an ideal tool for microsurgical procedures, as the energy is easily focussed on the targeted area producing a controlled and precise hole consistent between operators.…”
Section: Laser-assisted Hatchingmentioning
confidence: 99%
“…Laser-assisted zona drilling was first reported in 1991 by two groups led by Tadir [53] and Palankar [54]. Laser presents an ideal tool for microsurgical procedures, as the energy is easily focussed on the targeted area producing a controlled and precise hole consistent between operators.…”
Section: Laser-assisted Hatchingmentioning
confidence: 99%
“…While other researchers have elected to pursue wavelengths of high absorption coefficient (8) and have recently reported alternative methods for delivering these particular laser pulses to the zona target by either using glass micropipettes filled with positive air pressure (with the 193-nm radiation) (9) or using specialized fibers or micropipettes (in the case of the 2.94-txm erbium:YAG laser) (10), we have chosen to focus our attention on other wavelengths. Possible difficulties with contact methods such as energy delivery to the target, the need for making use of a glass or salt-based instrument (some of them are toxic), and the necessity for maintaining physical contact between the delivery media (pipette or fiber) defeat most of the advantages in using laser light and essentially reduce the laser-based techniques to a mechanical/contact mode, similar to conventional PZD methods.…”
Section: Introductionmentioning
confidence: 99%
“…But the resulting damage in the ZP was quite inconsistent. ArF-excimer laser emtting at 193 nm was first reported to allow ZP drilling of mouse oocytes with no detectable heating or structural damage of the surroundings (Palanker et al, 1991). Emitting in UV-spectrum this cold light laser was strongly absorbed by water and had a penetration depth of < 1 µm.…”
Section: Presumptions For the Ideal Lasermentioning
confidence: 99%