Technique for Rapid, Small-Scale Analysis of Vitamin C Levels in Fruit and Application to a Tomato Mutant Collection

Stevens, Rebecca; Buret, Michel; Garchery, Cécile; Carretero, Yolande; Causse, Mathilde

doi:10.1021/jf061241e

Cited by 140 publications

(104 citation statements)

References 19 publications

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“…These mutants contain reduced levels of ascorbic acid, and no null mutants are found, presumably because plants without ascorbate would not be viable. After screening 118 M82 tomato mutant families, a few mutants with reduced or increased fruit ascorbic acid content compared to M82 have been identified (Stevens et al, 2006). Fruits from the 118 families contained between 6.1 and 31.4 mg ascorbic acid/100 g fresh weight, which is similar to the range of natural variation observed in the populations studied here.…”

Section: Variation Of Ascorbic Acid Levels In the Populationssupporting

confidence: 68%

Candidate Genes and Quantitative Trait Loci Affecting Fruit Ascorbic Acid Content in Three Tomato Populations

et al. 2007

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Fresh fruit and vegetables are a major source of ascorbic acid (vitamin C), an important antioxidant for the human diet and also for plants. Ascorbic acid content in fruit exhibits a quantitative inheritance. Quantitative trait loci (QTL) for ascorbic acid content have been mapped in three tomato populations derived from crosses between cultivated tomato varieties (Solanum lycopersicum accessions) and three related wild species or subspecies. The first population consists of a set of introgression lines derived from Solanum pennellii, each containing a unique fragment of the wild species genome. The second population is an advanced backcross population derived from a cross between a cultivated tomato and a Solanum habrochaites (formerly Lycopersicum hirsutum) accession. The third population is a recombinant inbred line population derived from the cross between a cherry tomato line and a large fruited line. Common regions controlling ascorbic acid content have been identified on chromosomes 2, 8, 9, 10, and 12. In general, the wild alleles increased ascorbic acid content, but some improvement could also be provided by S. lycopersicum. Most QTLs appeared relatively stable over years and in different environments. Mapping of candidate genes involved in the metabolism of ascorbic acid has revealed a few colocations between genes and QTLs, notably in the case of a monodehydroascorbate reductase gene and a QTL present in two of the populations on chromosome 9 (bin 9-D), and a previously mapped GDP-mannose epimerase and a QTL on chromosome 9 (bin 9-J).

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Section: Variation Of Ascorbic Acid Levels In the Populationssupporting

confidence: 68%

Candidate Genes and Quantitative Trait Loci Affecting Fruit Ascorbic Acid Content in Three Tomato Populations

et al. 2007

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“…Ascorbate and dehydroascorbate were measured separately from the metabolite profiling using a microplate assay as described by Stevens et al (2006) on the frozen pericarp material stored at 280°C. Extractions and assays were carried out in ice-cold 6% (w/v) TCA in triplicate.…”

Section: Ascorbic Acid Levelmentioning

confidence: 99%

Genome-Wide Association in Tomato Reveals 44 Candidate Loci for Fruit Metabolic Traits

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et al. 2014

Plant Physiology

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Genome-wide association studies have been successful in identifying genes involved in polygenic traits and are valuable for crop improvement. Tomato (Solanum lycopersicum) is a major crop and is highly appreciated worldwide for its health value. We used a core collection of 163 tomato accessions composed of S. lycopersicum, S. lycopersicum var cerasiforme, and Solanum pimpinellifolium to map loci controlling variation in fruit metabolites. Fruits were phenotyped for a broad range of metabolites, including amino acids, sugars, and ascorbate. In parallel, the accessions were genotyped with 5,995 single-nucleotide polymorphism markers spread over the whole genome. Genome-wide association analysis was conducted on a large set of metabolic traits that were stable over 2 years using a multilocus mixed model as a general method for mapping complex traits in structured populations and applied to tomato. We detected a total of 44 loci that were significantly associated with a total of 19 traits, including sucrose, ascorbate, malate, and citrate levels. These results not only provide a list of candidate loci to be functionally validated but also a powerful analytical approach for finding genetic variants that can be directly used for crop improvement and deciphering the genetic architecture of complex traits.

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“…Total and reduced ascorbates were measured according to the procedure described by Stevens et al (2006) with slight modifications. Briefly, ascorbate was extracted from 50 to 100 mg of frozen pericarp powder in 500 μL of cold 6% phosphoric acid.…”

Section: Ascorbate and Glutathione Assaysmentioning

confidence: 99%

Differences in Blossom-end Rot Resistance in Tomato Cultivars is Associated with Total Ascorbate rather than Calcium Concentration in the Distal End Part of Fruits <i>per se</i>

et al. 2018

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Calcium is widely accepted as the main factor responsible for blossom-end rot (BER) appearance in tomato (Solanum lycopersicum L.) fruit. However, reactive oxygen species (ROS), which can damage plant tissues have also been proposed to initiate BER appearance in tomatoes and other fruit-bearing vegetables. Ascorbate, the major antioxidant in tomato fruit, is generally lower during green fruit development, which corresponds to the stage of BER appearance. Accordingly, one hypothesis is that tomato cultivars with a lower susceptibility to BER under salt stress have higher ascorbate contents and thus better control of ROS levels. In this study, to clarify the relationship between BER incidence and oxidative stress, two BER resistant cultivars, 'Managua RZ' and 'House Momotaro' and one BER-susceptible cultivar 'Reiyoh', were cultivated under salinity or standard nutrient solution (control) conditions. Calcium, potassium, magnesium, total hydro-soluble antioxidants, and ascorbate concentrations were measured in the distal pericarp 1 to 2 days prior to symptom appearance and during symptom appearance in healthy and affected fruits. When salt stress was applied, only BER-resistant cultivars showed a significant increase in ascorbate contents prior to BER appearance as compared with their levels under the control condition. In contrast, pre-BER Ca concentrations. A similar tendency was also observed in apple "bitter pit". The ability to increase the fruit antioxidant capacity and maintain mineral balance under salt stress conditions may explain the resistance to BER development in highly resistant cultivars, probably by the avoidance of oxidative-induced cell necrosis and stabilization of the cell membranes, respectively.

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Technique for Rapid, Small-Scale Analysis of Vitamin C Levels in Fruit and Application to a Tomato Mutant Collection

Cited by 140 publications

References 19 publications

Candidate Genes and Quantitative Trait Loci Affecting Fruit Ascorbic Acid Content in Three Tomato Populations

Candidate Genes and Quantitative Trait Loci Affecting Fruit Ascorbic Acid Content in Three Tomato Populations

Genome-Wide Association in Tomato Reveals 44 Candidate Loci for Fruit Metabolic Traits

Differences in Blossom-end Rot Resistance in Tomato Cultivars is Associated with Total Ascorbate rather than Calcium Concentration in the Distal End Part of Fruits <i>per se</i>

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