Techniques for intrinsically labeling wheat with zinc-65

Starks, Thomas L.; Johnson, Phyllis E.

doi:10.1021/jf00064a032

Cited by 28 publications

(19 citation statements)

References 13 publications

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“…However, in the matured grain only the percentage of B-hordein showed significant positive correlation with grain Zn concentration, whereas C-hordein showed a negative correlation (Figure S2A). In wheat, labelling studies with 65 Zn have shown that the glutenins have a much greater level of 65 Zn incorporation (47–65%) than the other grain proteins [16]. Similar results were obtained from barley storage protein extracts and it is reported that western immunoblotting assay shows the presence of B-hordein within this MW range [40].…”

Section: Discussionsupporting

confidence: 70%

Effects of Zn Fertilization on Hordein Transcripts at Early Developmental Stage of Barley Grain and Correlation with Increased Zn Concentration in the Mature Grain

2014

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Zinc deficiency is causing malnutrition for nearly one third of world populations. It is especially relevant in cereal-based diets in which low amounts of mineral and protein are present. In biological systems, Zn is mainly associated with protein. Cereal grains contain the highest Zn concentration during early developmental stage. Although hordeins are the major storage proteins in the mature barley grain and suggested to be involved in Zn binding, very little information is available regarding the Zn fertilization effects of hordein transcripts at early developmental stage and possible incorporation of Zn with hordein protein of matured grain. Zinc fertilization experiments were conducted in a greenhouse with barley cv. Golden Promise. Zn concentration of the matured grain was measured and the results showed that the increasing Zn fertilization increased grain Zn concentration. Quantitative real time PCR showed increased level of total hordein transcripts upon increasing level of Zn fertilization at 10 days after pollination. Among the hordein transcripts the amount of B-hordeins was highly correlated with the Zn concentration of matured grain. In addition, protein content of the matured grain was analysed and a positive linear relationship was found between the percentage of B-hordein and total grain Zn concentration while C-hordein level decreased. Zn sensing dithizone assay was applied to localize Zn in the matured grain. The Zn distribution was not limited to the embryo and aleurone layer but was also present in the outer part of the endosperm (sub-aleurone layers) which known to be rich in proteins including B-hordeins. Increased Zn fertilization enriched Zn even in the endosperm. Therefore, the increased amount of B-hordein and decreased C-hordein content suggested that B-hordein upregulation or difference between B and C hordein could be one of the key factors for Zn biofortification of cereal grains due to the Zn fertilization.

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Section: Discussionsupporting

confidence: 70%

Effects of Zn Fertilization on Hordein Transcripts at Early Developmental Stage of Barley Grain and Correlation with Increased Zn Concentration in the Mature Grain

2014

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“…Starks and Johnson (1985) showed that the majority of Zn applied to bread wheat at anthesis was incorporated into grain protein and that the greatest proportion of the 65 Zn applied was found in the glutenin. In the present study, foliar spray of all Zn fertilizers increased grain protein content of wheat plants over those of control plants.…”

Section: Discussionmentioning

confidence: 99%

The effectiveness of foliar applications of synthesized zinc-amino acid chelates in comparison with zinc sulfate to increase yield and grain nutritional quality of wheat

Ghasemi

Khoshgoftarmanesh

Afyuni

et al. 2013

European Journal of Agronomy

107

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“…A series of studies using radioactive Fe and Zn were carried out by nutritionists to determine the best ways to produce labeled food materials for animal feeding studies (table 1) [4][5][6][7][8][9][10]. These studies showed that micronutrients can be translocated to seeds from various tissues, hinting at a pathway that includes root, stem, leaf, and reproductive tissues.…”

Section: Which Tissues Supply Micronutrients To Seeds and At Which Dmentioning

confidence: 99%

Moving micronutrients from the soil to the seeds: Genes and physiological processes from a biofortification perspective

2011

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Techniques for intrinsically labeling wheat with zinc-65

Cited by 28 publications

References 13 publications

Effects of Zn Fertilization on Hordein Transcripts at Early Developmental Stage of Barley Grain and Correlation with Increased Zn Concentration in the Mature Grain

Effects of Zn Fertilization on Hordein Transcripts at Early Developmental Stage of Barley Grain and Correlation with Increased Zn Concentration in the Mature Grain

The effectiveness of foliar applications of synthesized zinc-amino acid chelates in comparison with zinc sulfate to increase yield and grain nutritional quality of wheat

Moving micronutrients from the soil to the seeds: Genes and physiological processes from a biofortification perspective

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