Techniques for measuring and manipulating free Ca2+ in the cytosol and organelles of neutrophils

Hallett, Maurice Bartlett; Hodges, Robert S.; Cadman, Martin; Blanchfield, Helen; Dewitt, Sharon; Pettit, Elizabeth J.; Laffafian, Iraj; Davies, E. V.

doi:10.1016/s0022-1759(99)00167-2

Cited by 32 publications

(21 citation statements)

References 36 publications

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“…The intracellular free calcium concentration ([Ca(2þ)](i)) was measured with flow cytometry (Hallett et al, 1999). Samples were analyzed after a washing step in PBS using the FACScalibur ® flow cytometer (Becton Dickinson) with an argon laser at a fixed output wavelength of 488 nm.…”

Section: Analysis Of Drug Dependence Using a Naloxone-induced Withdramentioning

confidence: 99%

Pharmacological characterization of conotoxin lt14a as a potent non-addictive analgesic

Ren

Wang

Qin

et al. 2015

Toxicon

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Section: Analysis Of Drug Dependence Using a Naloxone-induced Withdramentioning

confidence: 99%

Pharmacological characterization of conotoxin lt14a as a potent non-addictive analgesic

Ren

Wang

Qin

et al. 2015

Toxicon

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“…Cytosolic Ca 2+ + levels at the end of reperfusion were measured using the fluorescent Ca 2+ + indicator Fluo-3/AM as described 40 . Cardiomyocytes were incubated with 5 μM Fluo-3 at 37� C for 30 min in the dark.…”

Section: Cytosolic Ca 2+ + Level Measurementmentioning

confidence: 99%

Influence of ginsenoside-Re against myocardial infarction in isolated heart

Kim

Lim

Kang

et al. 2011

Mol. Cell. Toxicol.

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Ginsenosides, one of the well-known traditional herbal substance, frequently are used in South Korea for the prevention of cardiovascular disease. The effects of ginsenoside-Re (G-Re) on ischemia-induced heart were investigated through analyses of hemodynamic change including perfusion pressure, aortic flow, coronary flow, and cardiac output. Myocardial function was recorded. Administration of ginsenoside-Re significantly prevented decreases in perfusion pressure, aortic flow, coronary flow, and cardiac output. The Administration of G-Re significantly recovered all of the hemodynamic parameters, except heart rate, after ischemia-reperfusion (I/R) injury (perfusion pressure: 78.49±3.98% vs. 66.61±3.3%, aortic flow volume: 59.26±2.29% vs. 42.68±2.17%, coronary flow volume: 74.89±2.88% vs. 52.89±2.81%, and cardiac output: 63.12±3.81% vs. 45.25±3.83%, P⁄0.01, respectively, normal control as 100%). The intracellular calcium ([Ca 2+ ] i ) content in cardiomyocytes was quantitatively determined. Administration of G-Re significantly prevented [Ca 2+ ] i increase that had been induced by simulated I/R in vitro (P⁄0.01) in a dose-dependent manner, suggesting that the antiischemic role of G-Re is mediated by the inhibition of [Ca 2+ ] i increase.Over all, we found that the administration of G-Re has cardioprotective effects on the isolated heart. The beneficial effects may be mediated through controlling the level of [Ca 2+ ] i . These results indicate that G-Re has a distinct cardioprotective effect in an isolated rat heart.The primary cause of death worldwide 1,2 is ischemic cardiac disease (ICD). Myocardial infarction (MI) is the manifestation of a cardiac injury following coronary occulsion. When an MI is cured with early revascularization, this conditions leads to cardiac reperfusion. ischemia-reperfusion (I/R) is related with myocardial ischemia. When coronary flow is restored, the harmful effects occur causing oxidative stress and intracellular calcium increase, which lead to cell death 3 .The primary evidence of cardiac I/R are myocyte death and contractile dysfunction 4-6 . Individual surviving an MI are susceptible to cardiac dysfunction and sudden death 7 . During the last three decades, many development has been made in the cure of cardiac infarction, and these progress have decreased death from ICD 8-10 . While I/R injury limits cardiac salvage, reperfusion therapies that are protective in animal heart models have not yet been many advanced 11 . However, many mechanisms have been proposed that may relate cardiac I/R injury. Intracellular Ca 2+ ([Ca 2+ ] i ) is a important signal for controlling cellular functional level, including cellular survival and death 12 . While a small amount of [Ca 2+ ] i is need for optimal cellular function, showing obvious evidence suggests that an increased cytosolic Ca 2+ is one of the major contributors to car-

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“…Calcium mobilization was measured as described elsewhere [35]. In brief, sensitized BMMC were loaded with 5 mM Fura-2AM dissolved in Tyrode's/BSA at 37°C for 30 min.…”

Section: Calcium Mobilization and Nfat Transcription Factor Activitymentioning

confidence: 99%

“…Since some tyrosine kinase-inhibitors are able to halt β-hexosaminidase release [35], we tested the effect of SOS pretreatment on antigen induced-tyrosine phosphorylation of proteins in mast cells. Neither of the two SOS dilutions tested (i.e.…”

Section: Sos Pretreatment Does Not Affect Fcεri-induced Tyrosine Phosmentioning

confidence: 99%

Super-oxidized solution inhibits IgE-antigen-induced degranulation and cytokine release in mast cells

Medina-Tamayo

Sánchez-Miranda

Balleza-Tapia

et al. 2007

International Immunopharmacology

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Techniques for measuring and manipulating free Ca2+ in the cytosol and organelles of neutrophils

Cited by 32 publications

References 36 publications

Pharmacological characterization of conotoxin lt14a as a potent non-addictive analgesic

Pharmacological characterization of conotoxin lt14a as a potent non-addictive analgesic

Influence of ginsenoside-Re against myocardial infarction in isolated heart

Super-oxidized solution inhibits IgE-antigen-induced degranulation and cytokine release in mast cells

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