Techniques for the Characterization of UDP‐Glucuronyltransferase, Glucose‐6‐Phosphatase, and Other Tightly‐Bound Microsomal Enzymes

Zakim, David; Vessey, Donald A.

doi:10.1002/9780470110416.ch1

Cited by 59 publications

(1 citation statement)

References 62 publications

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“…The latency was about 5 %, which indicates that the assayed G6Pase activity reflects the total G-6-phosphohydrolase activity rather than the activity of the enzyme in intact microsornes (20). The interference of nonspecific phosphatases was negligible as there was no detectable hydrolysis of glycerol-2-phosphate in the assay conditions (21 al. (22).…”

Section: Hepatic Microsomal Enzyme Activitymentioning

confidence: 99%

Hepatic Glucose‐6‐Phosphatase Activity in Non‐Insulin Dependent Diabetics

Sotaniemi

Stengård

Saarni

et al. 1984

Journal of Internal Medicine

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The role of glucose‐6‐phosphatase (G6Pase) in postreceptional glucose handling in non‐insulin dependent diabetics (NIDDs) was investigated by comparing the enzyme values in diagnostic liver biopsy samples with fasting blood glucose (BG), immunoreactive insulin (IRI) and plasma antipyrine half‐life (T/2). The NIDDs, treated with sulphonylureas, had elevated serum aminotransferase and alkaline phosphatase values associated with fatty liver with or without fibrosis. G6Pase activity was reduced in the NIDDs compared with subjects who had undergone gallstone surgery (p<0.001), insulin dependent diabetics (p<0.001), and age‐ and sex‐matched non‐diabetics (p<0.001). G6Pase was inversely related to BG and antipyrine T/2, but not to IRI or conventional liver function tests. Therapy with phenobarbital and medroxyprogesterone acetate, known inducers, increased G6Pase activity, shortened antipyrine T/2, reduced BG and did not alter IRI, in four NIDDs. Low liver G6Pase activity in NIDDs may hence be one factor underlying the impaired glycemic control.

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Section: Hepatic Microsomal Enzyme Activitymentioning

confidence: 99%

Hepatic Glucose‐6‐Phosphatase Activity in Non‐Insulin Dependent Diabetics

Sotaniemi

Stengård

Saarni

et al. 1984

Journal of Internal Medicine

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Enzyme profile of rat bile ductular epithelial cells in reference to the resistance phenotype in hepatocarcinogenesis†

et al. 1989

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An extensive bile ductular cell hyperplasia with the formation of well-differentiated bile ductules is the most prominent feature of rat liver at 6 to 15 weeks after bile duct ligation. We have improved our previous cell isolation procedure and are now routinely able to obtain from such livers high yields of viable bile ductular epithelial cells. These cells were characterized with respect to their specific activities of gamma-glutamyl transpeptidase and beta-glucuronidase and of select Phase I and Phase II enzymes of biotransformation. At the time of their isolation, only a very small number of the bile ductular epithelial cells were observed to be in DNA synthesis. In addition, in histological sections prepared from intact hyperplastic bile ductular tissue isolates, only the bile ductular epithelial cells exhibited histochemical staining for gamma-glutamyl transpeptidase activity. Typically, greater than 95% of the cells isolated from this tissue were also found to be histochemically positive for gamma-glutamyl transpeptidase activity, and no hepatocytes were seen contaminating this cell population. Biochemically, the isolated bile ductular cells exhibited a gamma-glutamyl transpeptidase specific activity that was 100 times higher than that of hepatocytes isolated at the same time from the bile duct-ligated rats and more than 300 times higher than the specific activity of the enzyme of freshly isolated normal rat hepatocytes.(ABSTRACT TRUNCATED AT 250 WORDS)

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The Importance of Phospholipid-Protein Interactions for Regulation of the Activities of Membrane-Bound Enzymes

Zakim

Vessey

1980

Principles of Metabolic Control in Mammalian Systems

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Techniques for the Characterization of UDP‐Glucuronyltransferase, Glucose‐6‐Phosphatase, and Other Tightly‐Bound Microsomal Enzymes

Cited by 59 publications

References 62 publications

Hepatic Glucose‐6‐Phosphatase Activity in Non‐Insulin Dependent Diabetics

Hepatic Glucose‐6‐Phosphatase Activity in Non‐Insulin Dependent Diabetics

Enzyme profile of rat bile ductular epithelial cells in reference to the resistance phenotype in hepatocarcinogenesis†

The Importance of Phospholipid-Protein Interactions for Regulation of the Activities of Membrane-Bound Enzymes

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