Techniques for the chronic cannulation of the jugular vein in mice

Barr, James E.; Holmes, D.; Ryan, Lawrence J.; Sharpless, Seth K.

doi:10.1016/0091-3057(79)90307-1

Cited by 53 publications

(32 citation statements)

References 6 publications

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“…Briefly, a 6 cm length of SILASTIC tubing (0.25 mm inner diameter, 0.76 mm outer diameter) was fitted to a 26 gauge stainless steel cannula that was bent at a right angle and then embedded in a cement disk with an underlying nylon mesh. The catheter tubing was inserted 1.2 cm into the right external jugular vein and anchored with suture (Barr et al, 1979). In some cases, implantation in the right jugular vein was unsuccessful because of vein constriction during the procedure, in which case the catheter was implanted in the left jugular vein.…”

Section: Methodsmentioning

confidence: 99%

Lack of Self-Administration of Cocaine in Dopamine D₁Receptor Knock-Out Mice

Caine¹,

Thomsen²,

Gabriel³

et al. 2007

J. Neurosci.

170

134

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Evidence suggests a critical role for dopamine in the reinforcing effects of cocaine in rats and primates. However, self-administration has been less often studied in the mouse species, and, to date, "knock-out" of individual dopamine-related genes in mice has not been reported to reduce the reinforcing effects of cocaine. We studied the dopamine D 1 receptor and cocaine self-administration in mice using a combination of gene-targeted mutation and pharmacological tools. Two cohorts with varied breeding and experimental histories were tested, and, in both cohorts, there was a significant decrease in the number of D 1 receptor knock-out mice that met criteria for acquisition of cocaine self-administration (

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Section: Methodsmentioning

confidence: 99%

Lack of Self-Administration of Cocaine in Dopamine D₁Receptor Knock-Out Mice

Caine¹,

Thomsen²,

Gabriel³

et al. 2007

J. Neurosci.

170

134

View full text Add to dashboard Cite

show abstract

“…The duration of pumping (T) was calculated by the program according to the Eq. (1): (1) where: D = cocaine unit dose (nmol kg −1 ), W = body weight (kg), C = standard concentration of cocaine solution (nmol ml −1 ), F = flow rate (ml s −1 ).…”

Section: Animal Trainingmentioning

confidence: 99%

“…Detailed description of the surgical implantation of indwelling intravenous catheters can be found in the literature, for example, for rats [2,6], for mice [1,7,8], for monkeys [17,18,21], and for pigeons [14]. Briefly, animals were anesthetized with halothane.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Real time computation of in vivo drug levels during drug self-administration experiments

Tsibulsky

Norman

2005

Brain Research Protocols

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A growing body of evidence suggests that the drug concentration in the effect compartment of the body is the major factor regulating self-administration behavior. A novel computer-based protocol was developed to facilitate studies on mechanisms of drug addiction by determining correlations between drug levels and behavior during multiple drug injections and infusions. The core of the system is a user's program written in Medstate Notation language® (Med-Associates, Inc.), which runs the self-administration session (with MED-PC® software and hardware, Med-Associates, Inc.) and calculates the levels of infused and/or injected drugs in real time during the session. From the comparison of classical exponential and simple linear models of first-order kinetics, it is concluded that exponential solutions for the appropriate differential equations may be replaced with linear equations if the cycle of computation is much shorter than the shortest half-life for the drug. The choice between particular computation equations depends on assumptions about the pharmacokinetics of the particular drug: (i) one-, two-or three-compartment model, (ii) zero-, first-or second-order process of elimination, (iii) the constants of distribution and elimination half-lives of the drug are known or can be reasonably assumed, (iv) dependence of the constants on the drug level, and (v) temporal stability of all parameters during the session. This method of drug level computation can be employed not only for self-administration but also for other behavioral paradigms to advance pharmacokinetic/pharmacodynamic modeling.

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“…In brief, silicone rubber tubing (catalog number 11-189-15A, 0.51-mm inner diameter ϫ 0.94-mm outer diameter, Fisher) filled with saline was inserted into the jugular vein, and the outer portion of the tubing was tunneled subcutaneously, exiting at the nape of the neck. The length of tubing inserted into the jugular vein was 5 mm (38). Once the tubing was in place, it was filled with saline to maintain patency and was closed at the end with a metal stopper.…”

Section: Methodsmentioning

confidence: 99%

Regulation of Hepatic Apolipoprotein B-lipoprotein Assembly and Secretion by the Availability of Fatty Acids

Zhang

Hernandez‐Ono

et al. 2004

Journal of Biological Chemistry

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The in vivo effects of increased delivery of fatty acids (FA) to the liver are poorly defined. Therefore, we compared the effects of infusing either 6 mM oleic acid (OA) bound to albumin, 0.5-20% Intralipid, or saline for 3 or 6 h into male C57BL/6J mice. Infusions were followed by studies of triglyceride (TG) and apoB secretion. Although plasma FA levels increased similarly after either 20% Intralipid or 6 mM OA, TG secretion increased only after infusion of 4 -20% Intralipid; TG secretion was unchanged by 6 mM OA. By contrast, 6-h infusions of either 6 mM OA or 4 -20% Intralipid increased apoB secretion. 6 mM OA and 20% Intralipid each increased secretion of apoB from primary hepatocytes ex vivo. Importantly, 0.5-2% Intralipid, which delivered more FA to the liver than 6 mM OA, did not stimulate apoB secretion. Hepatic apoB mRNA levels were unaffected by either 6 mM OA or 20% Intralipid, but microsomal triglyceride transfer protein mRNA was significantly lower after 6-h infusions with 6 mM OA versus either saline or 20% Intralipid. Lower microsomal triglyceride transfer protein mRNA levels were associated with reduced hepatic TG mass after 6-h infusions of 6 mM OA. We conclude that 1) increased FA delivery to the liver in vivo increases secretion of apoBlipoproteins via post-transcriptional mechanisms, 2) OA-induced apoB-lipoprotein secretion occurred at least in part via mechanisms other than by providing substrate for TG synthesis, and 3) the route of delivery of FA is important for its effects on apoB secretion.Regulation of the assembly and secretion of apolipoprotein B (apoB)-lipoproteins and, in particular, very low density lipoprotein (VLDL), 1 by the liver has been studied intensively for many years in cultured hepatocytes (1-4). Many laboratories have also conducted in vivo studies of apoB-lipoprotein secretion in humans and animal models (5-7). However, despite the presence of a large body of work, several questions remain incompletely answered.First and foremost is the question of whether increased flux of plasma fatty acids (FA) to the liver can stimulate VLDL secretion. Studies in cultured liver cell lines, primary hepatocytes, and perfused livers have provided conflicting results relative to the role of FA delivery in VLDL secretion. Thus, several (8 -13), but not all (14 -16) studies in cultured liver cell lines support the proposal that apoB secretion is increased by increased FA uptake. Studies in primary hepatocytes from fed rats (16 -18) or hamsters (19,20) have failed to show that exogenous FA stimulate secretion of apoB. However, we were able recently to demonstrate oleic acid (OA)-induced apoB secretion in primary hepatocytes from fasted mice (21). Results from perfused rat livers have also been mixed; OA had no effect on apoB secretion in chow-fed rats (22) but increased apoB secretion in fasted (22) or high carbohydrate diet-fed (23) rats. In vivo studies in humans have also been inconclusive. Lewis et al. (24,25) demonstrate that, when they increased the plasma levels of FA by infusin...

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Techniques for the chronic cannulation of the jugular vein in mice

Cited by 53 publications

References 6 publications

Lack of Self-Administration of Cocaine in Dopamine D₁Receptor Knock-Out Mice

Lack of Self-Administration of Cocaine in Dopamine D₁Receptor Knock-Out Mice

Real time computation of in vivo drug levels during drug self-administration experiments

Regulation of Hepatic Apolipoprotein B-lipoprotein Assembly and Secretion by the Availability of Fatty Acids

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Techniques for the chronic cannulation of the jugular vein in mice

Cited by 53 publications

References 6 publications

Lack of Self-Administration of Cocaine in Dopamine D1Receptor Knock-Out Mice

Lack of Self-Administration of Cocaine in Dopamine D1Receptor Knock-Out Mice

Real time computation of in vivo drug levels during drug self-administration experiments

Regulation of Hepatic Apolipoprotein B-lipoprotein Assembly and Secretion by the Availability of Fatty Acids

Contact Info

Product

Resources

About

Lack of Self-Administration of Cocaine in Dopamine D₁Receptor Knock-Out Mice

Lack of Self-Administration of Cocaine in Dopamine D₁Receptor Knock-Out Mice