Techniques for the detection and analysis of LLPS and MLOs

Antifeeva, Iuliia A.; Fonin, Alexander V.; Fefilova, Anna S.; Mokin, Yakov I.; Silonov, Sergey A.; Uversky, Vladimir N.; Turoverov, Konstantin К.; Kuznetsova, Irina М.

doi:10.1016/b978-0-12-823967-4.00025-7

Cited by 1 publication

(1 citation statement)

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“…This process segregates proteins and nucleic acids into specialized micron-sized bodies known as biomolecular condensates or membraneless organelles (MLOs), which lack membranes but exhibit liquid-like properties [25]. MLOs are capable of rapid assembly and disassembly in response to stimuli and play an important role in cellular spatial-temporal organization [26]. The change in biopolymers' molecular concentration is the main driving force of LLPS [27].…”

Section: Pml Regulation At the Mrna Levelmentioning

confidence: 99%

PML Body Biogenesis: A Delicate Balance of Interactions

Silonov,

Smirnov,

Kuznetsova

et al. 2023

IJMS

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PML bodies are subnuclear protein complexes that play a crucial role in various physiological and pathological cellular processes. One of the general structural proteins of PML bodies is a member of the tripartite motif (TRIM) family—promyelocytic leukemia protein (PML). It is known that PML interacts with over a hundred partners, and the protein itself is represented by several major isoforms, differing in their variable and disordered C-terminal end due to alternative splicing. Despite nearly 30 years of research, the mechanisms underlying PML body formation and the role of PML proteins in this process remain largely unclear. In this review, we examine the literature and highlight recent progress in this field, with a particular focus on understanding the role of individual domains of the PML protein, its post-translational modifications, and polyvalent nonspecific interactions in the formation of PML bodies. Additionally, based on the available literature, we propose a new hypothetical model of PML body formation.

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Section: Pml Regulation At the Mrna Levelmentioning

confidence: 99%