Techniques in Breeding for Resistance to Blueberry Stem Blight Caused by Botryosphaeria Dothidea

Cline, W. O.; Milholland, R. D.; Rooks, S.D.; Ballington, James R.

doi:10.17660/actahortic.1993.346.14

Cited by 10 publications

(17 citation statements)

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“…It is important to minimise the likelihood of using isolates that might give false resistance readings due to lack of virulence. Virulence may be affected by strain, age of the culture, number of subcultures taken and spore production method (Cline 1993;Smith 2004;Polashock 2006;Polashock et al 2006). The objective of this study was to develop a rapid, in vitro method for producing virulent conidia of Neofusicoccum spp.…”

Section: Introductionmentioning

confidence: 99%

Comparison of in vitro and in planta sporogenesis in Neofusicoccum species from blueberry

Yang

Turner

Stephens

et al. 2017

NZPP

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Stem diseases are an economically important problem in the production of blueberry (Vaccinium spp.). The diseases cause significant crop loss, including the death of entire bushes. Resistance phenotyping assays require large numbers of conidia. A fast and reliable in vitro method to mass produce viable and pathogenic Neofusicoccum spores would be more suitable than the current in planta methods. Two strains each of N. parvum and N. ribis were used to develop a new in vitro method, which involved interrupting mycelial mats produced on agar plates, with wet and dry cycles. Spores were generated at room temperature after 7-12 days' incubation. Spore production varied among replicate tests and the four isolates used. Neofusicoccum ribis isolate Nr175 (LUPP1348) generated the most spores in the shortest time. Pathogenicity assays using spores generated in vitro suggest that these spores are more virulent and pathogenic than those produced in planta.

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Section: Introductionmentioning

confidence: 99%

Comparison of in vitro and in planta sporogenesis in Neofusicoccum species from blueberry

Yang

Turner

Stephens

et al. 2017

NZPP

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“…Stem blight resistance is a primary objective of most southern blueberry breeding programs Cline et al, 1993;Gupton and Smith, 1989), and selection of resistant genotypes generally relies on natural infection of seedlings in field trials (Buckley,1990;Creswell and Milholland, 1987). A detached stem assay showed good correlation with natural field infections and with artificially inoculated plants in the field, greenhouse, and laboratory of rabbiteye blueberry cultivars (Smith, 1997).…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Southern Highbush Blueberry Cultivars to Botryosphaeria Stem Blight

Smith¹

2004

Small Fruits Review

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Stem blight, caused by the fungus Botryosphaeria dothidea, is a destructive disease of rabbiteye (Vaccinium ashei Reade) and highbush (V. corymbosum L.) blueberries in the southeastern United States. The susceptibility of 20 southern highbush, two rabbiteye, and two highbush cultivars were compared using a detached stem assay. Fresh isolates of B. dothidea obtained from infected southern highbush blueberry plants were used as inoculum in this study. Succulent, partiallyhardened stems were surface disinfected, rinsed in sterile distilled water, wounded by scraping away a section of bark, and inoculated by covering the wound with a mycelial agar block of B. dothedia and securing with parafilm wrap. The base of each stem was inserted into moistened, sterilized sand in a 150 mm ϫ 25 mm tissue culture tube and incubated at 25°C, 100% RH for 30 days. Lesion length was measured after 15 days incubation. Cultivars with the shortest mean lesion length were classified as relatively resistant and included 'Pearl

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“…Since B. dothidea isolates rapidly lose virulence in culture (Cline et al, 1993), fresh isolates were obtained each year from symptomatic 2-to 4year-old southern highbush blueberry cultivars growing in research plots in Pearl River County, Mississippi about one month before the initiation of each study. Five millimeter internal stem pieces were cut from the edge of lesions, surface sterilized, rinsed in sterile distilled water, and placed on acidified potato dextrose agar (A-PDA).…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…Stein blight resistance is a primary objective of most southern blueberry breeding programs (Ballington et al, 1993;Cline et al, 1993;Gupton and Smith, 1989;Lyrene, 2002;Rooks et al, 1995). Selection of resistant genotypes generally relies on natural infection of seedlings in field trials (Buckley, 1990;Creswell and Milholland, 1987); however, several protocols have been used to screen large numbers of blueberry seedlings for stern blight resistance (Cline et al, 1993: Creswell andMilholland.…”

Section: Introductionmentioning

confidence: 99%

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Botryosphaeria Stem Blight of Southern Blueberries: Cultivar Susceptibility and Effect of Chemical Treatments

Smith¹

2009

Acta Hortic.

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Botryosphaeria stem blight, caused by the fungus Botryosphaeria dothidea, is a destructive disease of rabbiteye (Vacciniu,n ache!) and highbush (V. coiy,nbosun) blueberries in the southeastern United States. The susceptibility of 21 southern highhush and seven rabbiteye blueberry cultivars was compared using a detached stem assay. Succulent, partially-hardened stems were wounded, inoculated with a m ycelial block from a 14-day-old culture of B. dothidea, and incubated for 15 days. Disease severity was determined by comparing lesion length with that of the susceptible rabbiteye cultivar Tifblue. Cultivars with a mean lesion length less than that of 'Tifblue' were classified as resistant and included three southern highhush cultivars: Pearl River, Emerald, and Star. Cultivars whose lesion length was equal to or greater than that of Tifhlue' in both years were classified as susceptible or very susceptible and included eight southern highbush cultivars: Legacy. Gulf Coast, Cooper, Jubilee, Biloxi, O'Neal, Magnolia, andReveille. In a comparison of wound versus non-wound inoculations, lesion length was significantly greater on stems wounded by removing a small section of hark prior to inoculation than on nonwounded stems or on stems inoculated at a fresh leaf scar. The potential efficacy of fungicides for stem blight control was determined using the detached stem assay. In two studies stems treated with cyprodinil + tiudioxonil or pyraclostrobin had shorter lesions than stems receiving no fungicide treatment while stems treated with captan, Aram, and tebuconazole had lesions as long as those receiving no fungicide treatment.

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Techniques in Breeding for Resistance to Blueberry Stem Blight Caused by Botryosphaeria Dothidea

Cited by 10 publications

References 0 publications

Comparison of <i>in vitro</i> and in planta sporogenesis in <i>Neofusicoccum</i> species from blueberry

Comparison of <i>in vitro</i> and in planta sporogenesis in <i>Neofusicoccum</i> species from blueberry

Susceptibility of Southern Highbush Blueberry Cultivars to Botryosphaeria Stem Blight

Botryosphaeria Stem Blight of Southern Blueberries: Cultivar Susceptibility and Effect of Chemical Treatments

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