Techno-economic assessment of high-solid simultaneous saccharification and fermentation and economic impacts of yeast consortium and on-site enzyme production technologies

Khajeeram, Sutamat; Unrean, Pornkamol

doi:10.1016/j.energy.2017.01.090

Cited by 39 publications

(6 citation statements)

References 35 publications

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“…Experiments using enzymatic consortium from fungi demonstrated a greater production of cellulases and efficiency on saccharification compared to the performance of an enzymatic extract from a single microorganism (Zhang et al, 2009). Recent studies using cellulases/ hemicellulases mixtures from different fungal species have been developed in order to increase the enzymatic potential on the saccharification, mainly from Trichoderma reesei and Penicillium chrysosporium (Khajeeram and Unrean, 2017;Lin et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Rodrigues

Pereira

Santos

et al. 2017

Industrial Crops and Products

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This study evaluated the synergistic efficiency of crude enzymatic extracts (CEE) produced by fungal strains Aspergillus fumigatus SCBM6 (AFE) and Aspergillus niger SCBM1 (ANE) in the hydrolysis of alkali pretreated sugarcane bagasse (2% NaOH (w v −1), ∼100°C, 4 h and liquid-to-solid ratio of 25 (v w −1)). CEEs were produced by solid-state fermentation (SSF) and presented high concentration of β-glucosidase (AFE = 78.4 U g −1 and ANE = 43.02 U g −1), β-xylosidase (ANE = 78.0 U g −1) and xylanase (AFE = 1774.5 U g −1 and ANE = 3289.6 U g −1). A 2 4 full design of experiments was used to optimize the hydrolysis of pretreated bagasse as a function of time, temperature, amount of pretreated bagasse and AFE-to-ANE ratio. After hydrolysis optimization, the maximum concentration of total reducing sugars (TRS) was 21.68 g L −1. It was also noticed a significant production of xylose (135.59 mg) and arabinose (58.10 mg) per gram of hemicelluloses and a slight production of glucose (20.18 mg) per gram of cellulose. As fungi consortium were excellent producers of hemicellulases, it is suggested that they could be used for pentoses saccharification and production of ethanol via C-5 fermentation, xylitol, biopolymers, among other bioproducts.

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Section: Introductionmentioning

confidence: 99%

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Rodrigues

Pereira

Santos

et al. 2017

Industrial Crops and Products

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“…The profitability of all biofuel processes is marginal resulting in a process with high uncertainty. This implies that any saving in the energy demand operating cost would be critical to the future commercialization of this technology 42,45‐47 . The fiber recovery from the PMS is an easy process step and does not require any heat input 9,15,44,48 .…”

Section: Resultsmentioning

confidence: 99%

“…This implies that any saving in the energy demand operating cost would be critical to the future commercialization of this technology. 42,[45][46][47] The fiber recovery from the PMS is an easy process step and does not require any heat input. 9,15,44,48 The inorganic materials encapsulating the fibrous can be readily washed out using a low concentrated (diluted) acid or by mechanical separation.…”

Section: Sludge Identitymentioning

confidence: 99%

Techno‐economic analysis and a novel assessment technique of paper mill sludge conversion to bioethanol toward sustainable energy production

et al. 2020

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A novel bioprocess design to convert paper mill sludge (PMS) to biofuels is proposed in this work. The design utilizes cellulosic fiber recovered from the PMS via optimized de-ashing (HCl washing) step. This work specifically provided a technical and economic analysis of paper mill sludge conversion into biofuel production using a novel protocol. The protocol is based on scanning electron microscopy (SEM) analysis to assess the quality of the contained cellulose prior to further processing. The results are crucially important to determine the suitability of the PMS feedstock to produce biofuels. SEM analysis was employed as a preliminary screening tool to evaluate sludge digestibility and conversion. The SEM characterization technique established a direct relationship between the fiber morphology, presence of crystals salts and sugar yield after enzymatic hydrolysis. Substantial structural changes were observed before and after de-ashing the sludge samples, leading to a correlation between the surface morphology and the washing step. The results suggested that deashing changes the surface morphology and upon analysis, increased the sugar yield up to about 86% as opposed to 2.2% in sludge sample A as an example. The PMS conversion into biofuel was simulated using Aspen PLUS and compared to a similar process using corn stover as feedstock. The simulation results showed it is 20% cheaper to produce bioethanol from PMS compared to corn stover. The simulation revealed less energy demand by around 13 320 MJ/h compared to that when corn stover was used.

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“…The fermentation process is the process of converting glucose into ethanol with the help of yeast. The most tried yeast in the bioethanol fermentation process is Saccharomyces cerevisiae and Scheffersomyces stipitis as done by Mohd Azhar et al as well as a consortium of both in the research of Khajeeram dan Unrean (2017) [30], [31]. In addition, organic acids, ammonia, and sulfides formed during fermentation can also act as inhibitors [32], [33].…”

Section: Bioethanolmentioning

confidence: 99%

“…In addition, microorganisms cannot ferment hexose and pentose sugars at the same time. To answer this problem, hybrid microorganisms are used which are the result of genetic engineering or from the use of joint cultures of two types of microorganisms [31], [33].…”

Section: Bioethanolmentioning

confidence: 99%

The potential of dewaka banana stems as raw materials for bioethanol

Suryaningsih

Mursalim

Laga

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Increasing production and consumption of fossil fuels encourage the use of renewable energy. Renewable energy that does not have the potential to disrupt the stability of the availability of other materials is the use of waste. One type of waste that has been researched into bioethanol is banana waste in the form of stems, leaves, skins, and weevil. Dewaka banana is one type of banana that grows in Merauke, has a large enough size so that it has the potential to produce a lot of waste and tastes sour. Dewaka banana production in 2019 was 7,087 tons and produced waste in the form of Dewaka banana stems as much as 28,546.96 tons. The water content of Dewaka banana stems is 93.42%, and taking into account the yield loss of ± 10%, there will be 1,690.55 tons of dry waste. The amount of waste can produce 148,092.27 liters of bioethanol in a year based on the best research results from Roni, et al. Dewaka banana stems can be processed and reused into bioethanol as an alternative energy raw material.

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Techno-economic assessment of high-solid simultaneous saccharification and fermentation and economic impacts of yeast consortium and on-site enzyme production technologies

Cited by 39 publications

References 35 publications

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Techno‐economic analysis and a novel assessment technique of paper mill sludge conversion to bioethanol toward sustainable energy production

The potential of dewaka banana stems as raw materials for bioethanol

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