Technological challenges of biomembrane-coated top-down cancer nanotherapy

Ravasco, João M. J. M.; Paiva‐Santos, Ana Cláudia; Conde, João

doi:10.1038/s44222-022-00008-2

Cited by 11 publications

(12 citation statements)

References 10 publications

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“…However, the scope of super homotypic targeting extends far beyond these applications, offering enormous potential across various biological and medical fields. In cancer therapy 5,10,13 , it could enable the precise targeting of tumor cells, minimizing damage to healthy tissue, which in turn improves treatment outcomes and lessens adverse effects. In tissue engineering 14,15 , it may foster more effective tissue regeneration and repair by promoting the growth and organization of specific cell types, crucial for healing wounds or replacing damaged organs.…”

Section: Discussionmentioning

confidence: 99%

Super homotypic targeting by exosome surface engineering

Wang,

Ding,

Liu

et al. 2024

Preprint

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Homotypic targeting is the inherent ability of cells for preferential interaction with cells of similar or identical types, a phenomenon commonly seen in cell adhesion, tissue formation, and immune responses. Unfortunately, its full potential remains largely untapped. Here we introduce an approach to drastically boost the homotypic targeting capabilities of cells via exosomes (nanoscale extracellular vesicles secreted by cells). By engineering exosome surfaces with lanthanides, we amplify specific cell-exosome interactions by more than 25-fold, significantly accelerating the selective capture of exosomes by cells of the same lineage. This substantial enhancement in cellular homophilicity opens up an entirely new class of applications, two of which we showcase here with unprecedented performance: using cells to detect specific exosomes and using exosomes to detect specific cells. The concept of super homotypic targeting offers enormous potential to transform cancer diagnostics, immunotherapy, targeted drug delivery, tissue engineering, and vaccine development.

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Section: Discussionmentioning

confidence: 99%

Super homotypic targeting by exosome surface engineering

Wang,

Ding,

Liu

et al. 2024

Preprint

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“…Top-down cell membrane processing uses existing cell membranes and allows lipid membranes to be merged with cell membranes for use as vaccine delivery vehicles . Cell membrane-based nanovaccines, which apply cell membranes to nanoparticles, may enable programmable immune responses. , Cell membrane-based nanoparticle vaccine delivery methods are promising, although concerns over the biocompatibility and suitability of these technologies exist. , First, the development of cell membrane-based platforms requires large-scale manufacturing and purification capacities.…”

Section: Introductionmentioning

confidence: 99%

Mammalian Cell Membrane Hybrid Polymersomes for mRNA Delivery

Shin,

Ahn,

Kim

et al. 2024

ACS Appl. Mater. Interfaces

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Cell membranes are structures essential to the cell function and adaptation. Recent studies have targeted cell membranes to identify their protective and interactive properties. Leveraging these attributes of cellular membranes and their application to vaccine delivery is gaining increasing prominence. This study aimed to fuse synthetic polymeric nanoparticles with cell membranes to develop cell membrane hybrid polymersomes (HyPSomes) for enhanced vaccine delivery. We designed a platform to hybridize cell membranes with methoxy-poly(ethylene glycol)-block-polylactic acid nanoparticles by using the properties of both components. The formed HyPSomes were optimized by using dynamic light scattering, transmission electron microscopy, and Forster resonance energy transfer, and their stability was confirmed. The synthesized HyPSomes replicated the antigenic surface of the source cells and possessed the stability and efficacy of synthetic nanoparticles. These HyPSomes demonstrated enhanced cellular uptake and translation efficiency and facilitated endosome escape. HyPSomes showed outstanding capabilities for the delivery of foreign mRNAs to antigen-presenting cells. HyPSomes may serve as vaccine delivery systems by bridging the gap between synthetic and natural systems. These systems could be used in other contexts, e.g., diagnostics and drug delivery.

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“…This promising cell-mimicking approach enables NPs to acquire the inherent biological properties of progenitor cell membranes. By covering NPs with a natural cell membrane, the antigenic profile and interfacial properties of the progenitor cell can be faithfully preserved and transferred to the abiotic NPs [ 4 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Bioengineered exosomal-membrane-camouflaged abiotic nanocarriers: neurodegenerative diseases, tissue engineering and regenerative medicine

Lopes¹,

Lopes²,

Pereira-Silva³

et al. 2023

Military Med Res

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A bio-inspired strategy has recently been developed for camouflaging nanocarriers with biomembranes, such as natural cell membranes or subcellular structure-derived membranes. This strategy endows cloaked nanomaterials with improved interfacial properties, superior cell targeting, immune evasion potential, and prolonged duration of systemic circulation. Here, we summarize recent advances in the production and application of exosomal membrane-coated nanomaterials. The structure, properties, and manner in which exosomes communicate with cells are first reviewed. This is followed by a discussion of the types of exosomes and their fabrication methods. We then discuss the applications of biomimetic exosomes and membrane-cloaked nanocarriers in tissue engineering, regenerative medicine, imaging, and the treatment of neurodegenerative diseases. Finally, we appraise the current challenges associated with the clinical translation of biomimetic exosomal membrane-surface-engineered nanovehicles and evaluate the future of this technology.

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Technological challenges of biomembrane-coated top-down cancer nanotherapy

Cited by 11 publications

References 10 publications

Super homotypic targeting by exosome surface engineering

Super homotypic targeting by exosome surface engineering

Mammalian Cell Membrane Hybrid Polymersomes for mRNA Delivery

Bioengineered exosomal-membrane-camouflaged abiotic nanocarriers: neurodegenerative diseases, tissue engineering and regenerative medicine

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