Technological process for cell disruption, extraction and encapsulation of astaxanthin from Haematococcus pluvialis

Machado, Francisco R.S.; Trevisol, Thalles Canton; Boschetto, Daiane L.; Burkert, J. F. M.; Ferreira, Sandra R.S.; Oliveira, J. Vladimir; Burkert, Carlos André Veiga

doi:10.1016/j.jbiotec.2015.12.004

Cited by 55 publications

(33 citation statements)

References 36 publications

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“…Under these conditions, AX microcapsulation yield and EE reached 38.02% and 71.76%, respectively. Machado Jr. et al () stated that the EE of AX from H. pluvialis was 51.21%.…”

Section: Resultsmentioning

confidence: 99%

Astaxanthin from Haematococcus pluvialis Microencapsulated by Spray Drying: Characterization and Antioxidant Activity

Zhao

Liu

Zhang

et al. 2018

J Americ Oil Chem Soc

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Astaxanthin (AX) is an unstable functional food ingredient. A stable AX powder was developed and its characteristics (such as moisture content, bulk density, solubility, repose angle, and morphology) and antioxidant activity were evaluated. The microencapsulated AX powder was produced by spray drying using maltodextrin (MD)-gelatin and the parameters were optimized by response surface methodology. The results revealed that an optimal microencapsulation process has a ratio of MD to gelatin of 2.1:1, a ratio of wall to core materials of 5.9:1, and a ratio of glycerol monostearate to sucrose fatty-acid ester of 1.1:1. The AX encapsulation yield and encapsulation efficiency were 38.02% and 71.76%, respectively. AX microcapsules had a lower moisture content and bulk density, greater solubility, and good flowability. AX microcapsules showed antioxidant activities greater than Vitamin C, which indicated that the antioxidant activity of AX was not lost. AX microcapsule micrographs showed almost no cracks or fissures on the surface of microcapsules produced by spray drying under the optimal conditions.

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“…Under these conditions, AX microcapsulation yield and EE reached 38.02% and 71.76%, respectively. Machado Jr. et al () stated that the EE of AX from H. pluvialis was 51.21%.…”

Section: Resultsmentioning

confidence: 99%

Astaxanthin from Haematococcus pluvialis Microencapsulated by Spray Drying: Characterization and Antioxidant Activity

Zhao

Liu

Zhang

et al. 2018

J Americ Oil Chem Soc

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“…In terms of astaxanthin extraction from yeast cells, Michelon et al [41] combined maceration with diatomaceous earth and Glucanex lysis, and this process resulted in 122% extraction yield of carotenoids from Phaffia rhodozyma, compared to DMSO extraction. In another study, enzymatic cell lysis using different types of enzymes (Glucanex, Lyticase, and Driselase) was combined with ultrasound pre-treatment to extract astaxanthin inform the microalgae Haematococcus pluvialis [42]. This strategy resulted in 84% of astaxanthin extraction compared to standard chemical extraction methods.…”

Section: Supercritical Fluid Extraction As Means Of Astaxanthin Extramentioning

confidence: 99%

Optimised Production and Extraction of Astaxanthin from the Yeast Xanthophyllomyces dendrorhous

et al. 2020

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Currently, astaxanthin demand is fulfilled by chemical synthesis using petroleum-based feedstocks. As such, alternative pathways of natural astaxanthin production attracts much research interest. This study aimed at optimising bioreactor operation parameters for astaxanthin production and evaluating strategies for its subsequent extraction. The effect of pH and agitation was evident, as a significant reduction in both biomass and astaxanthin production was observed when the culture pH was not controlled and a low agitation speed was applied. At controlled pH conditions and a high agitation speed, a significant increase in biomass (16.4 g/L) and astaxanthin production (3.6 mg/L) was obtained. Enzymatic yeast cell lysis using two commercial enzymes (Accellerase 1500 and Glucanex) was optimised using the central composite design of experiment (DoE). Accellerase 1500 led to mild cell disruption and only 9% (w/w) astaxanthin extraction. However, Glucanex treatment resulted in complete astaxanthin extractability, compared to standard extraction method (DMSO/acetone). When supercritical CO 2 was employed as an extraction solvent in Accellerase-pre-treated Xanthophyllomyces dendrorhous cells, astaxanthin extraction increased 2.5-fold. Overall, the study showed that extraction conditions can be tailored towards targeted pigments present in complex mixtures, such as in microbial cells.Microorganisms 2020, 8, 430 2 of 18 up and commercialisation of astaxanthin production [3-5], while its extraction and purification still contributes to the overall complexity and cost of the whole production process.Various cultivation modes, including batch, fed-batch, and continuous, have been investigated for carotenoid production in yeasts, either in the lab or at the pilot scale [6][7][8]. In the case of some yeast species cultivated on batch mode, high initial carbon concentrations (usually glucose) result in suppression of cell growth as well as product formation due to the Crabtree effect [9,10]. It has been demonstrated that X. dendrorhous undergoes the Crabtree effect, a phenomenon where cells metabolically switch to fermentative metabolism leading to ethanol production even under ample oxygen supply when initial glucose present is above a given threshold (strain dependent) [10,11]. Fed-batch cultivation mode is considered as an appropriate strategy to overcome such issues, as it allows the addition of one or more nutrients to the reactor during fermentation in order to maintain the concentration of the substrate below its inhibitory levels [12]. Furthermore, with regards to media optimization, low concentrations of ammonium and phosphate have been reported to be favourable towards astaxanthin production, whereas increased levels of citrate in the growth medium have been shown to stimulate astaxanthin production, as citrate can act as a carbon source towards astaxanthin biosynthesis in Phaffia rhodozyma [13]. The effect of light on astaxanthin productivity in several yeast and microalgae strains has been also extensively studied [1...

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“…Carbohydrase activity and specificity have demonstrated potential in targeting the polysaccharide-rich What DSP techniques can be utilized to recover and fractionate products? nature of Nannochloropsis and Haematococcus cell walls (Machado et al 2016;Zuorro et al 2015). Likewise, Chlamydomonas, Chlorella, and Spirulina species possess multilayered cell walls primarily composed of metabolites including proteins, lipids, and polysaccharides (not including cellulose).…”

Section: Future Directions Of Aqueous Enzymatic Processingmentioning

confidence: 99%

Green microalgae biomolecule separations and recovery

Dixon

Wilken

2018

Bioresour. Bioprocess.

116

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Microalgae biomass has garnered significant attention as a renewable energy feedstock and alternative to petroleumbased fuels. The diverse metabolism of green microalgae species additionally provides opportunities for recovery of products for feed, food, nutraceutical, cosmetic, and biopharmaceutical industries. Recently, the concept of using microalgae as part of a biorefinery model has been adopted in place of refinery methods focused on recovering one target product. This has led to producers exploring co-production of high value and high volume products in an effort to improve process economics. With numerous potential products and applications, the biomass source or specific strain must be carefully selected to accumulate extractable levels of the target molecule(s). It is additionally imperative to understand the morphology and metabolism of the selected strain to cost-effectively manage all stages of commercial production. This review will focus specifically on microalgae in the division of Chlorophyta, or green algae and their extracellular matrices (ECM), potential for commercial products, and finally describe a holistic approach for biomolecule extraction and recovery. Additionally, cell disruption and fractionation methods for recovery of biomolecules for commercial products are highlighted along with an alternative method, aqueous enzymatic processing for multiple biomolecule extraction and recovery from green microalgae. An emphasis is placed on connecting the morphological characteristics of microalgae ECM or organelle membranes to implications on separation and purification technologies.

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Technological process for cell disruption, extraction and encapsulation of astaxanthin from Haematococcus pluvialis

Cited by 55 publications

References 36 publications

Astaxanthin from Haematococcus pluvialis Microencapsulated by Spray Drying: Characterization and Antioxidant Activity

Astaxanthin from Haematococcus pluvialis Microencapsulated by Spray Drying: Characterization and Antioxidant Activity

Optimised Production and Extraction of Astaxanthin from the Yeast Xanthophyllomyces dendrorhous

Green microalgae biomolecule separations and recovery

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