Tegumental surface changes in adult Fasciola hepatica following treatment in vitro and in vivo with an experimental fasciolicide

Rivera, Norma; Ibarra, Froylán; Zepeda, Armando; Fortoul, Teresa I.; Hernández, Ariana; Castillo, Rafael; Canto, G. J.

doi:10.1007/s00436-004-1127-3

Cited by 22 publications

(22 citation statements)

References 15 publications

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“…The dorsal surface showed a similar disruption to the ventral surface and the posterior region was more severely disrupted than the anterior part. The findings are similar to the effects of praziquantel (Apinhasmit & Sobhon, 1996), albendazole sulphoxide (Buchanan et al, 2003), and 5-chloro-2-methylthio-6-(1-naphthyloxy)-1-H-benzimidazole (Rivera et al, 2004(Rivera et al, , 2005. The regional differences in damage may be due to tegumental structure, route of drug uptake and drug metabolism (McKinstry et al, 2003).…”

Section: Discussionsupporting

confidence: 72%

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

et al. 2007

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The effect of niclosamide on the tegument of adult Haplorchis taichui (Trematoda: Heterophyidae) exposed in vitro was observed by scanning electron microscope. Adult worms were incubated in Tyrode's solution containing 0.01, 0.1, 1.0, and 10 mg ml 21 of niclosamide for 30 min, 1, 6, 12 and 24 h. Control groups were incubated in Tyrode's solution without niclosamide and worms remained active until 24 h. In 0.01 mg ml 21 of niclosamide, worms showed slightly active movements up to 1 h after incubation, while in 0.1 mg ml 21 solution a few worms showed only slightly active movements after 30 min. Tegumental changes were determined by scanning electron microscopy. Swelling and blebbing of the tegument were observed on both ventral and dorsal sides. After longer periods, extensive swelling and blebbing of the tegument became more severe and there was a loss of the apical plasma membrane in some regions. Empty spine sockets occurred, and small perforations penetrated the basal lamina, followed by some lesions. Destruction of both surfaces was more pronounced on the posterior compared with the anterior regions.

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Section: Discussionsupporting

confidence: 72%

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

et al. 2007

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“…AJPS are also caused by the effect of commercial fascioliscide; it can therefore be assumed that these extracts can cause similar effects [20][21][22]. With regard to the other fragments (ethyl acetate and methanol) no notorious fascioliscide activity was detected, since very limited activity (10%) was demonstrated during the testing of the extracts.…”

Section: Resultsmentioning

confidence: 99%

<i>In Vitro</i> Evaluation of Fasciolicide Activity with Hexane, Methanol and Ethyl Acetate with Extracts Processed and Obtained from Some Mexican Plants Used in Traditional Medicine Based on Ethno Botanical Studies

Ibarra-Moreno¹,

Ibarra-Velarde²,

Ávila-Acevedo³

2012

AJPS

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Fasciolosis is a parasitic disease of world distribution affecting mainly domestic ruminants. The control of this disease is carried out using chemical fasciolicides which, in some cases, have been observed to have environmental problem such us pollution resistance. An alternative is to investigate extracts from plants with anti-Fasciola hepatica effects, taking advantage of the great diversity of flora of our country. The aim of this paper is to identify, assess and elucidate the anti-Fasciola hepatica effect in vitro using antiparasitic extracts of some plants used in Mexican ethno botany. We collected, dried, processed and tested in vitro about 20 plants with their respective chemical elements (hexane, ethyl acetate and methanol), obtaining results of a total of 60 extracts tested. The in vitro evaluations were carried out for 3 days, and the efficacy of each extract was compared with an untreated control group. Each test was repeated 6 times with 13 extracts that showed greater fasciolocide activity. Results from these 13 extracts tested ranged from 80% to 100% activity and the plants tested were: Castela tortuosa (chaparro amargo), Achillea millefolium (plumajillo), Thymus vulgaris (thyme), Justicia spicigera (muicle), Limpia critridora (cedron), Populus alba (Alamo), Mentha piperita (mint), Chenopodium graveolens (epazote de zorrillo), Lippia graveolens (oregano), Artemisia mexicana (estafiate) and Artemisia absinthium (wormwood), which is the hexane variable which showed higher fasciolicide capacity; using a dose of 500 gr/Lt in all the trials. Further in vitro studies should be conducted to obtain the LD50 of each extract to be able to isolate the main active element found in the hexane variable.

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“…It has been firmly documented that one of the hallmark effects of any anthelmintic is the destruction of the worm's surface. This is due to the fact that the tegumental and/or cuticular structures are the primary parasite-host interfaces, vital for absorption of nutrients and perception of the surrounding micro-environment provided by the host (William et al 2001;Mckinstry et al 2003;Rivera et al 2004;Xiao et al 2004;Roy et al 2008). Histological studies have revealed that anthelmintic agents induce considerable changes in the internal structural features of helminths, resulting in loss of normal cellular conformity in vital tissues (Roy et al 2008;Dasgupta et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Potentilla fulgens (Family Rosaceae), a medicinal plant of north-east India: a natural anthelmintic?

et al. 2010

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The cestode parasite, Raillietina echinobothrida and the trematode, Gastrothylax crumenifer were exposed to the ethanolic root peel extract of Potentilla fulgens, an antiparasitic local medicinal plant of Meghalaya, India, to evaluate the anthelmintic efficacy of the plant. The parasites were incubated in 1, 5, 10, 20, 50 and 100 mg crude alcoholic extract per ml of phosphate buffered saline (PBS) at a temperature of 37 ± 1°C. Paralysis and death were observed at 2.00 ± 0.05 and 2.80 ± 0.06 h for the cestode and 1.21 ± 0.06 and 2.18 ± 0.04 h for the trematode parasites at the highest test concentration of the plant extract. The commercial anthelmintic, Praziquantel (PZQ) showed higher activity at the tested concentration (0.02 mg/ml). To further investigate the efficacy of the plant extract, vital tegumental enzymes of the parasite viz. Acid phosphatase (AcPase), Alkaline phosphatase (AlkPase) and Adenosine triphosphatase (ATPase) were studied. Quantitatively, the total enzyme activity of AcPase, AlkPase and ATPase was found to be reduced significantly by 69.20, 66.43 and 29.63% for R. echinobothrida and 47.96, 51.79 and 42.63% for G. crumenifer, respectively compared to the respective controls; histochemical study also showed reduction in the visible staining of the enzymes. The reference drug, PZQ also showed more or less similar effect like that of the plant extract. The result suggests that phytochemicals of P. fulgens have anthelmintic potential.

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Tegumental surface changes in adult Fasciola hepatica following treatment in vitro and in vivo with an experimental fasciolicide

Cited by 22 publications

References 15 publications

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

<i>In Vitro</i> Evaluation of Fasciolicide Activity with Hexane, Methanol and Ethyl Acetate with Extracts Processed and Obtained from Some Mexican Plants Used in Traditional Medicine Based on Ethno Botanical Studies

Potentilla fulgens (Family Rosaceae), a medicinal plant of north-east India: a natural anthelmintic?

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Tegumental surface changes in adult Fasciola hepatica following treatment in vitro and in vivo with an experimental fasciolicide

Cited by 22 publications

References 15 publications

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

&lt;i&gt;In Vitro&lt;/i&gt; Evaluation of Fasciolicide Activity with Hexane, Methanol and Ethyl Acetate with Extracts Processed and Obtained from Some Mexican Plants Used in Traditional Medicine Based on Ethno Botanical Studies

Potentilla fulgens (Family Rosaceae), a medicinal plant of north-east India: a natural anthelmintic?

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<i>In Vitro</i> Evaluation of Fasciolicide Activity with Hexane, Methanol and Ethyl Acetate with Extracts Processed and Obtained from Some Mexican Plants Used in Traditional Medicine Based on Ethno Botanical Studies