Telencephalon transcriptome analysis of chronically stressed adult zebrafish

Huang, Victoria; Butler, Anderson A.; Lubin, Farah D.

doi:10.1038/s41598-018-37761-7

Cited by 20 publications

(20 citation statements)

References 59 publications

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“…S4, and S5). Similarly to previous studies, we also found changes in the expression of interferon inducible proteins IFI6/IFI27-like associated genes and pathways 69 ( Supplementary Fig. S5).…”

Section: Discussionsupporting

confidence: 89%

“…2, Tables 2, 3). However, some other reports failed to evoke overt anxiety 69 , raising questions of whether CUS protocol was indeed properly applied in such studies (e.g., see 70,71 for a recent discussion of challenges with data reliability and replicability in zebrafish behavioral models). Thus, further research is needed, for example, to compare zebrafish that received CUS protocols that would differ in the numbers of stressors, their severity and/or duration.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Demin

Lakstygal

Krotova

et al. 2020

Sci Rep

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Stress-related neuropsychiatric disorders are widespread, debilitating and often treatment-resistant illnesses that represent an urgent unmet biomedical problem. Animal models of these disorders are widely used to study stress pathogenesis. A more recent and historically less utilized model organism, the zebrafish (Danio rerio), is a valuable tool in stress neuroscience research. Utilizing the 5-week chronic unpredictable stress (CUS) model, here we examined brain transcriptomic profiles and complex dynamic behavioral stress responses, as well as neurochemical alterations in adult zebrafish and their correction by chronic antidepressant, fluoxetine, treatment. Overall, CUS induced complex neurochemical and behavioral alterations in zebrafish, including stable anxiety-like behaviors and serotonin metabolism deficits. Chronic fluoxetine (0.1 mg/L for 11 days) rescued most of the observed behavioral and neurochemical responses. Finally, whole-genome brain transcriptomic analyses revealed altered expression of various CNS genes (partially rescued by chronic fluoxetine), including inflammation-, ubiquitin- and arrestin-related genes. Collectively, this supports zebrafish as a valuable translational tool to study stress-related pathogenesis, whose anxiety and serotonergic deficits parallel rodent and clinical studies, and genomic analyses implicate neuroinflammation, structural neuronal remodeling and arrestin/ubiquitin pathways in both stress pathogenesis and its potential therapy.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Demin

Lakstygal

Krotova

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Given our hypothesis that the enteric neurons further along a differentiation program were localized to the distal tip of Sub-Cluster 3, we asked whether this population of cells contained additional neurochemical or neuron subtype specific differentiation genes. As expected, a small pocket of these cells was found to contain the two acetylcholine associated genes, acetylcholine esterase (ache) (Bertrand et al, 2001;Huang et al, 2019) and vesicular acetylcholine transferase (slc18a3a) (Hong et al, 2013;Zoli, 2000) (Furness et al, 2004;Memic et al, 2018). Corroborating our single-cell findings, HCR analysis revealed the co-expression of pbx3b, calb2a, vipb, nos1, and slc18a3a in discrete differentiating enteric neurons within the foregut region of the zebrafish gut at 68 hpf ( Figure 5G).…”

Section: Sox10-derived Cells During the Embryonic To Early Larval Trasupporting

confidence: 84%

An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution

Howard

Baker

Ibarra‐García‐Padilla

et al. 2020

Preprint

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Neural crest cells (NCCs) are vertebrate stem cells that give rise to various cell types throughout the developing body in early life. Here, we utilized single-cell transcriptomic analyses to delineate NCC-derivatives along the posterior developing vertebrate, zebrafish, during the late embryonic to early larval stage, a period when NCCs are actively differentiating into distinct cellular lineages. We identified several major NCC/NCC- derived cell-types including mesenchyme, neural crest, neural, neuronal, glial, and pigment, from which we resolved over three dozen cellular subtypes. We dissected gene expression signatures of pigment progenitors delineating into chromatophore lineages, mesenchyme subtypes, and enteric NCCs transforming into enteric neurons. Global analysis of NCC derivatives revealed they were demarcated by combinatorial hox gene codes, with distinct profiles within neuronal cells. From these analyses, we present a comprehensive cell-type atlas that can be utilized as a valuable resource for further mechanistic and evolutionary investigations of NCC differentiation.

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“…Scientific RepoRtS | (2019) 9:17063 | https://doi.org/10.1038/s41598-019-53634-z www.nature.com/scientificreports www.nature.com/scientificreports/ changes in the HF diet-fed zebrafish. Recently, Huang et al34 performed RNA sequencing analysis of the telencephalon in chronically stressed adult zebrafish and revealed the altered expression of 155 genes, some of which are known to be critical for memory. Although this chronic stress treatment did not include dietary stress, we consider that the HF diet might also produce memory-associated gene expression changes in the telencephalon of zebrafish similar to that in mammals[28][29][30][31][35][36][37] .…”

mentioning

confidence: 99%

High-fat diet impairs cognitive function of zebrafish

Meguro

Hosoi

Hasumura

2019

Sci Rep

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An unhealthy diet with excessive fat intake has often been claimed to induce not only obesity but also cognitive dysfunction in mammals; however, it is not known whether this is the case in zebrafish. Here, we investigated the effect of excessive fat in the diet on cognitive function and on gene expression in the telencephalon of zebrafish. Cognitive function, as measured by active avoidance test, was impaired by feeding of a high-fat diet compared with a control diet. In RNA sequencing analysis of the telencephalon, 97 genes were identified with a fold change in expression greater than 2 and a p-value less than 0.05 between the two diets. In quantitative real-time PCR analysis of the telencephalon, genes related to neuronal activity, anti-oxidative stress, blood-brain barrier function and amyloid-β degradation were found to be downregulated, whereas genes related to apoptosis and amyloid-β production were found to be upregulated, in the high-fat diet group, which are changes known to occur in mammals fed a high-fat diet. Collectively, these results are similar to those found in mammals, suggesting that zebrafish can serve as a suitable animal model in research into cognitive impairment induced by excessive fat in the diet.According to the World Health Organization, in December 2017 around 50 million people had dementia, and 10 million new cases emerge every year 1 . Dementia is a syndrome-usually of a chronic or progressive nature-in which there is deterioration in cognitive function 1 . Some research has shown a relationship between the development of cognitive impairment and life-style-related risk factors that are shared with other non-communicable diseases 1 . Obesity and unhealthy diet are two risk factors for dementia 1,2 . Excessive fat intake has often been claimed to induce obesity in humans and rodents 3,4 . Although numerous studies have been conducted to elucidate the effects of obesity and excessive dietary fat intake on cognitive function in rodents [5][6][7] , no such studies have been conducted in zebrafish (Danio rerio).The organs and tissues of zebrafish are similar in terms of structure and function to those of humans. In addition, these fish are amenable to genetic manipulation, breed readily in captivity, and can be inexpensively maintained, making them a useful model organism for investigating many human pathological conditions 8 . Several reports suggest that zebrafish are a suitable model organism for examining the mechanisms underlying lipid metabolism leading to obesity. For example, overexpression of the endogenous melanocortin antagonist agouti-related protein or the serine/threonine protein kinase Akt1 results in the development of adiposity in zebrafish 9,10 . Overfeeding and a high-fat (HF) diet induce body fat accumulation in zebrafish through pathophysiological pathways common with those underlying mammalian obesity; furthermore, green tea extract and fish oil, which have well-known anti-obesity activities, can cancel out these effects. Thus, zebrafish are a useful model of human...

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Telencephalon transcriptome analysis of chronically stressed adult zebrafish

Cited by 20 publications

References 59 publications

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution

High-fat diet impairs cognitive function of zebrafish

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