Telomerase activity and expression of human telomerase RNA component and human telomerase reverse transcriptase in lung carcinomas

Kumaki, Fumiyuki; Kawai, Toshiaki; Hiroi, Sadayuki; Shinomiya, Nariyoshi; Ozeki, Yuichi; Ferrans, Víctor J.; Torikata, Chikao

doi:10.1053/hupa.2001.21567

Cited by 86 publications

(76 citation statements)

References 43 publications

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“…In line with previous studies we found that both the proportion of hTERT-positive samples and hTERT mRNA levels were higher in tumour samples than in paired normal lung samples of lung cancer patients (19,20,22,(26)(27)(28)(29)(30). However, despite the fact that we examined material of a large cohort of patients and found an association between clinical stage and hTERT mRNA positivity in tumour tissue, we were unable to confirm a previously reported association between RT-PCR detected elevated hTERT mRNA levels of tumour samples and a decreased disease-free survival (19,20,22).…”

Section: Discussionsupporting

confidence: 93%

“…Studies on the potentially prognostic value of telomerase activity or hTERT expression in tumours of surgically treated lung cancer patients have, however, yielded conflicting results (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32). The possibility that the level of hTERT mRNA and telomerase activity, rather than detectability of these parameters could have a predictive value might be one of the reasons for these discordant findings.…”

Section: Discussionmentioning

confidence: 99%

“…In patients with surgically-treated early-stage lung cancer a correlation has been found between a poor prognosis and detection in tumour tissue of either elevated hTERT expression [by RT-PCR (19)(20)(21)(22), immunohistochemistry (23) or ISH (24,25)] or telomerase activity (26)(27)(28)(29). However, data on this topic are not consistent since other studies failed to reveal a correlation between hTERT expression and (diseasefree) survival (28,30,31).…”

Section: Introductionmentioning

confidence: 99%

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Prognostic value of hTERT mRNA expression in surgical samples of lung cancer patients: the European Early Lung Cancer Project

et al. 2010

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prognostic value of hTERT mRNA expression in surgical samples of lung cancer patients: the European Early Lung Cancer Project

et al. 2010

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“…Such an association has been reported in mISH (Kumaki et al, 2001;Wang et al, 2002;Fujita et al, 2003;Lantuejoul et al, 2004) and immunohistochemistry studies (Kumaki et al, 2001;Toomey et al, 2001;Lantuejoul et al, 2004). In contrast, the prognostic value of hTERT mRNA expression assayed by RT -qPCR has been inconsistent Hara et al, 2001;Marchetti et al, 2002;Wu et al, 2003;Hsu et al, 2004).…”

Section: Discussionmentioning

confidence: 84%

Amplification of telomerase (hTERT) gene is a poor prognostic marker in non-small-cell lung cancer

et al. 2006

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Telomerase reactivation is a hallmark of human carcinogenesis. Increased telomerase activity may result from gene amplification and/or overexpression. This study evaluates the prognostic value of hTERT gene amplification and mRNA overexpression in 144 resectable non-small-cell lung cancer (NSCLC) specimens. The hTERT gene copy number was assessed by quantitative polymerase chain reaction (qPCR) on laser-capture microdissected tumour cells of 81 tumours, and by fluorescence in situ hybridisation (FISH) on a subset of 59 tumours. hTERT mRNA level was determined by reverse transcription (RT) -qPCR in 130 tumours. In total, 57% of (46 out of 81) primary NSCLC specimens demonstrated hTERT amplification, which was significantly more common (Po0.001) in adenocarcinoma (30 out of 40) than in squamous cell carcinoma (13 out of 37). The hTERT mRNA overexpression was noted in 74% (94 out of 130) of tumours; it was more frequent in squamous cell than in adenocarcinoma (87 vs 68%, P ¼ 0.03). Overexpression was significantly associated with amplification (P ¼ 0.03), especially in adenocarcinoma. The hTERT gene amplification was prognostic for shorter recurrence-free survival (hazard ratio ¼ 2.16, P ¼ 0.03). These data indicate that gene amplification is an important mechanism for hTERT overexpression in lung adenocarcinoma and is an independent poor prognostic marker for disease-free survival in NSCLC.

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“…Although the hTERT mRNA expression RQ-RT-PCR detection method that we used represents only an indicator of the expression level of telomerase mRNA, not a direct assay for the presence of active telomerase, some recent studies have shown a close correlation between hTERT expression and telomerase activity, the expression of hTERT subunit being the limiting factor for telomerase activity. 26,31,32 Moreover, the RQ-RT-PCR is the most sensitive, accurate, and adaptable RNA quantitative technique. 30 We showed that elevation of hTERT mRNA expression correlates with severity of histological changes, which is in general concordance with previous studies performed on surgical resection specimens or in a group of patients with a previous malignancy and unknown smoking status.…”

Section: Discussionmentioning

confidence: 99%

Loss of heterozygosity and human telomerase reverse transcriptase (hTERT) expression in bronchial mucosa of heavy smokers

Čapková

Kalinová

Krsková

et al. 2007

Cancer

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We performed surface modification of ultra‐high‐molecular‐weight polyethylene (UHMWPE) through chromic acid etching with the aim of improving the performance of UHMWPE's composites with poly(ethylene terephthalate) fibers. In part I of this study, we evaluated the effects of chemical modification on the surface properties of UHMWPE with X‐ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and contact angle measurements. The thermal properties, rheology, and sintering behavior of the modified UHMWPE were compared to those of the base material. XPS and FTIR analysis confirmed the presence of carboxyl and hydroxyl groups on the surface of the modified powders. The substitution of polar groups into the backbone of the polymer decreased its contact angles with water and hexadecane and increased its surface energy, as evidenced by contact angle measurements. The modified UHMWPE was more crystalline than the base resin and less prone to thermal degradation. Although the rheological properties were virtually identical, the modified powders sintered more readily, presumably due to their higher surface energy, which suggested enhanced processability by compression molding. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci, 2006

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Telomerase activity and expression of human telomerase RNA component and human telomerase reverse transcriptase in lung carcinomas

Cited by 86 publications

References 43 publications

Prognostic value of hTERT mRNA expression in surgical samples of lung cancer patients: the European Early Lung Cancer Project

Prognostic value of hTERT mRNA expression in surgical samples of lung cancer patients: the European Early Lung Cancer Project

Amplification of telomerase (hTERT) gene is a poor prognostic marker in non-small-cell lung cancer

Loss of heterozygosity and human telomerase reverse transcriptase (hTERT) expression in bronchial mucosa of heavy smokers

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